Abstract
The mechanism of nitrate reductase (NR) regulation under long-term anoxia in roots of whole plants and the putative role of nitrate in anoxia tolerance have been addressed. NR activity in tomato roots increased significantly after 24 h of anaerobiosis and increased further by 48 h, with a concomitant release of nitrite into the culture medium. Anoxia promoted NR activation through dissociation of the 14-3-3 protein inhibitor and NR dephosphorylation. After 24 h of anoxia, the total amount of NR increased slightly up to 48 h. However, NR-mRNA levels remained constant between 0 h and 24 h of root anoxia and decreased after 48 h. This is probably due to the inhibition of NR degradation and the accumulation of its native form. NR was slightly dephosphorylated in the absence of oxygen and nitrate. Under anoxia, NR dephosphorylation was modulated by nitrate-controlled NR activity. In addition, the presence of nitrate prevents anoxic symptoms on leaves and delays wilting by 48 h during root anoxia. In the absence of nitrate, plants withered within 24 h, as they did with tungstate treatment, an inhibitor of NR activity. Thus, anoxia tolerance of tomato roots could be enhanced by nitrate reduction.
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Claims 13
Claim · model-written summary
Tomato root NR activity rose after 24 hours of anaerobiosis and further by 48 hours.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- rootse0
- Treatment
- anaerobiosise0
- Gene
- NRe0
- Outcome
- NR activitye0
- Direction
- increasede0
- Qualifier
- after 24 h of anaerobiosis and increased further by 48 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NR activity in tomato roots increased significantly after 24 h of anaerobiosis and increased further by 48 h, with a concomitant release of nitrite into the culture medium.”
Claim · model-written summary
Anaerobiosis coincided with nitrite release into the culture medium.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- release of nitrite into the culture mediume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NR activity in tomato roots increased significantly after 24 h of anaerobiosis and increased further by 48 h, with a concomitant release of nitrite into the culture medium.”
Claim · model-written summary
Anoxia promoted NR activation through dissociation of its inhibitor and dephosphorylation.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Anoxiae0
- Gene
- NRe0
- Outcome
- NR activatione0
- Direction
- promotede0
- Qualifier
- dissociation of the 14-3-3 protein inhibitor and NR dephosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Anoxia promoted NR activation through dissociation of the 14-3-3 protein inhibitor and NR dephosphorylation.”
t0“tomato”
Claim · model-written summary
Total NR amount rose slightly from 24 to 48 hours of anoxia.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- anoxiae0
- Gene
- NRe0
- Outcome
- total amount of NRe0
- Direction
- increasede0
- Qualifier
- After 24 h of anoxiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 24 h of anoxia, the total amount of NR increased slightly up to 48 h.”
t0“tomato”
Claim · model-written summary
NR-mRNA stayed constant through 24 hours of root anoxia.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- NR-mRNAe0
- Outcome
- NR-mRNA levelse0
- Direction
- remained constante0
- Qualifier
- between 0 h and 24 h of root anoxiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NR-mRNA levels remained constant between 0 h and 24 h of root anoxia and decreased after 48 h.”
t0“tomato”
Claim · model-written summary
NR-mRNA decreased after 48 hours of root anoxia.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- NR-mRNAe0
- Outcome
- NR-mRNA levelse0
- Direction
- decreasede0
- Qualifier
- after 48 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NR-mRNA levels remained constant between 0 h and 24 h of root anoxia and decreased after 48 h.”
t0“tomato”
Claim · model-written summary
The authors suggest inhibited NR degradation and native-form accumulation explain the amount change.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- NRe0
- Outcome
- accumulation of its native forme0
- Qualifier
- inhibition of NR degradatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is probably due to the inhibition of NR degradation and the accumulation of its native form.”
t0“tomato”
Claim · model-written summary
NR was slightly dephosphorylated without oxygen and nitrate.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- absence of oxygen and nitratee0
- Gene
- NRe0
- Outcome
- NR was slightly dephosphorylatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NR was slightly dephosphorylated in the absence of oxygen and nitrate.”
t0“tomato”
Claim · model-written summary
Under anoxia nitrate-controlled NR activity modulated NR dephosphorylation.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- anoxiae0
- Gene
- NRe0
- Outcome
- NR dephosphorylatione0
- Qualifier
- nitrate-controlled NR activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under anoxia, NR dephosphorylation was modulated by nitrate-controlled NR activity.”
t0“tomato”
Claim · model-written summary
Nitrate prevented anoxic leaf symptoms and delayed wilting by 48 hours during root anoxia.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- presence of nitratee0
- Outcome
- anoxic symptoms on leaves and delays wiltinge0
- Direction
- preventse0
- Value
- 48 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of nitrate prevents anoxic symptoms on leaves and delays wilting by 48 h during root anoxia.”
t0“tomato”
Claim · model-written summary
Without nitrate plants withered within 24 hours.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- absence of nitratee0
- Outcome
- plants witherede0
- Value
- 24 he0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the absence of nitrate, plants withered within 24 h, as they did with tungstate treatment, an inhibitor of NR activity.”
t0“tomato”
Claim · model-written summary
Tungstate-treated plants also withered within 24 hours.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- tungstate treatmente0
- Outcome
- plants witherede0
- Value
- 24 he0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the absence of nitrate, plants withered within 24 h, as they did with tungstate treatment, an inhibitor of NR activity.”
t0“tomato”
Claim · model-written summary
The authors suggest nitrate reduction could enhance tomato root anoxia tolerance.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- anoxia tolerance of tomato rootse0
- Direction
- enhancede0
- Qualifier
- nitrate reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anoxia tolerance of tomato roots could be enhanced by nitrate reduction.”
The same questions in other papers
nitrogen · disease · Solanum lycopersicum (tomato)Positive and negative results
18 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
- negative
30 papers more in this group
See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.