Abstract
The effect of cytoplasmic polyamines on peribacteroid membrane transport processes in soybean ( Glycine max L.) was investigated. The concentration of free polyamines in soybean nodule cytoplasm has been estimated by others to be in the micromolar range. The H+ -ATPase was inhibited by 37 and 54% by 200 µM spermidine and putrescine, respectively. Spermine applied to the cytoplasmic face of the peribacteroid membrane was found to inhibit both inward and outward currents through a non-selective cation channel permeable to ammonium ( K d 2.1 µM at –100 mV). Malate transport into intact symbiosomes was reduced by 15–30% by 15 mM spermidine, cadaverine and putrescine. A non-specific stimulation of malate transport by polycations was found to occur at concentrations in the micromolar range. The results suggest that polyamines can affect all the peribacteroid membrane transport processes tested. In particular, we conclude that the combined inhibitory effects of polyamines on the ATPase and the ammonium channel have the potential to reduce nitrogen supply to the plant in vivo . The possibility of competing polyamine and ureide synthesis in the nodule is discussed.
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Claims 8
Claim · model-written summary
At 200 µM, spermidine inhibited H+-ATPase by 37%.
- Taxon
- soybean
Glycine maxe1 - Treatment
- 200 µM spermidinee0
- Gene
- H+ -ATPasee0
- Outcome
- H+ -ATPasee0
- Direction
- inhibitede0
- Value
- 37e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The H+ -ATPase was inhibited by 37 and 54% by 200 µM spermidine and putrescine, respectively.”
e1“soybean ( Glycine max L.)”
Claim · model-written summary
At 200 µM, putrescine inhibited H+-ATPase by 54%.
- Taxon
- soybeanGlycine maxe1
- Treatment
- putrescinee0
- Gene
- H+ -ATPasee0
- Outcome
- H+ -ATPasee0
- Direction
- inhibitede0
- Value
- 54%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The H+ -ATPase was inhibited by 37 and 54% by 200 µM spermidine and putrescine, respectively.”
e1“soybean ( Glycine max L.)”
Claim · model-written summary
Spermine inhibited inward and outward currents through a non-selective cation channel.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- peribacteroid membranee0
- Treatment
- Sperminee0
- Outcome
- inward and outward currentse0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Spermine applied to the cytoplasmic face of the peribacteroid membrane was found to inhibit both inward and outward currents through a non-selective cation channel permeable to ammonium ( K d 2.1 µM at –100 mV).”
e1“soybean ( Glycine max L.)”
Claim · model-written summary
At 15 mM, spermidine reduced malate transport into symbiosomes by 15–30%.
- Taxon
- soybeanGlycine maxe1
- Treatment
- 15 mM spermidinee0
- Outcome
- Malate transport into intact symbiosomese0
- Direction
- reducede0
- Value
- 15–30%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malate transport into intact symbiosomes was reduced by 15–30% by 15 mM spermidine, cadaverine and putrescine.”
e1“soybean ( Glycine max L.)”
Claim · model-written summary
At 15 mM, cadaverine reduced malate transport into symbiosomes by 15–30%.
- Taxon
- soybeanGlycine maxe1
- Treatment
- cadaverinee0
- Outcome
- Malate transport into intact symbiosomese0
- Direction
- reducede0
- Value
- 15–30%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malate transport into intact symbiosomes was reduced by 15–30% by 15 mM spermidine, cadaverine and putrescine.”
e1“soybean ( Glycine max L.)”
Claim · model-written summary
At 15 mM, putrescine reduced malate transport into symbiosomes by 15–30%.
- Taxon
- soybeanGlycine maxe1
- Treatment
- putrescinee0
- Outcome
- Malate transport into intact symbiosomese0
- Direction
- reducede0
- Value
- 15–30%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malate transport into intact symbiosomes was reduced by 15–30% by 15 mM spermidine, cadaverine and putrescine.”
e1“soybean ( Glycine max L.)”
Claim · model-written summary
Micromolar polycations stimulated malate transport nonspecifically.
- Taxon
- soybeanGlycine maxe1
- Treatment
- polycationse0
- Outcome
- malate transporte0
- Direction
- stimulatione0
- Qualifier
- concentrations in the micromolar rangee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A non-specific stimulation of malate transport by polycations was found to occur at concentrations in the micromolar range.”
e1“soybean ( Glycine max L.)”
Claim · model-written summary
Combined ATPase and ammonium-channel inhibition by polyamines could reduce nitrogen supply to soybean in vivo.
- Taxon
- soybeanGlycine maxe1
- Treatment
- polyaminese0
- Outcome
- nitrogen supply to the plante0
- Direction
- reducee0
- Qualifier
- have the potentiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combined inhibitory effects of polyamines on the ATPase and the ammonium channel have the potential to reduce nitrogen supply to the plant in vivo”
e1“soybean ( Glycine max L.)”
The same questions in other papers
spermidine · molecular: metabolites · Glycine max (soybean)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
putrescine · molecular: metabolites · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.