Paper · 2014 · Scientific Papers - Series A, Agronomy

EFFECT OF DROUGHT STRESS BY USING PEG 6000 ON GERMINATION AND EARLY SEEDLING GROWTH OF Brassica juncea Var. Ensabi

A. F. Toosi; Baki Bin Bakar; Mehdi Azizi

10
claims
20
verbatim quotes
15
citations
1
research areas
—
Not open access

Abstract

To study on effect of PEG 6000 stress on germination and early seedling growth of B. juncea (L.) Czern. var. Ensabi an experiment was conducted in the laboratories of Institute of Biological Sciences, University of Malaya, Malaysia. Seed germination and final germination rate of B. juncea var. Ensabi significantly affected by PEG 6000 concentration. By increasing osmotic potential of PEG 6000, seed germination and final germination were decreased. In distilled water, percentage of seed germination was highest. The higher amount of PEG 6000 concentration in this research (10 and 12 MPa) completely inhibited seed germination. Signs of germination for control and low PEG 600 osmotic potential occurred between 24 and 48 hours after sowing but at higher PEG 6000 osmotic solution it was being later. Most complete germination occurred at 25 o -30 o C. In PEG 6000 solution, the hypocotyl lengths of seedlings decreased with an increase in water stress. Shoots elongation significantly decreased by concentration of 2-8 MPa whereas no hypocotyl elongation at concentration of 10 and 12 MPa and shoot elongation completely inhibited. Keyword: Ensabi, germination, temperature, PEG 6000 concentration, hypocotyls.

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Claims 10

Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

PEG 6000 concentration significantly affected seed germination and final germination rate.

Taxon
B. juncea var. EnsabiBrassica junceae0
Treatment
PEG 6000 concentratione0
Outcome
Seed germination and final germination ratee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed germination and final germination rate of B. juncea var. Ensabi significantly affected by PEG 6000 concentration”

✓ located verbatim in the source · abstract, characters 233–350 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Increasing PEG 6000 osmotic potential decreased seed germination and final germination.

Taxon
B. juncea var. EnsabiBrassica junceae1
Treatment
PEG 6000e0
Outcome
seed germination and final germinatione0
Direction
decreasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“By increasing osmotic potential of PEG 6000, seed germination and final germination were decreased”

✓ located verbatim in the source · abstract, characters 352–450 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed germination percentage was highest in distilled water.

Taxon
B. juncea var. EnsabiBrassica junceae1
Treatment
distilled watere0
Outcome
percentage of seed germinatione0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In distilled water, percentage of seed germination was highest”

✓ located verbatim in the source · abstract, characters 452–514 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

PEG 6000 at 10 and 12 MPa completely inhibited seed germination.

Taxon
B. juncea var. EnsabiBrassica junceae1
Treatment
PEG 6000 concentration in this research (10 and 12 MPa)e0
Outcome
seed germinatione0
Direction
completely inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“PEG 6000 concentration in this research (10 and 12 MPa) completely inhibited seed germination”

✓ located verbatim in the source · abstract, characters 537–630 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Germination appeared at 24 to 48 hours for control and low PEG osmotic potential, and later at higher PEG 6000 osmotic solution.

Taxon
B. juncea var. EnsabiBrassica junceae1
Treatment
higher PEG 6000 osmotic solutione0
Outcome
Signs of germinatione0
Direction
latere0
Value
24 and 48 hourse0
Comparator
control and low PEG 600 osmotic potentiale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Signs of germination for control and low PEG 600 osmotic potential occurred between 24 and 48 hours after sowing but at higher PEG 6000 osmotic solution it was being later”

✓ located verbatim in the source · abstract, characters 632–803 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Most complete germination occurred at 25 o -30 o C.

Taxon
B. juncea var. EnsabiBrassica junceae1
Outcome
Most complete germinatione0
Value
25 o -30 o Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Most complete germination occurred at 25 o -30 o C”

✓ located verbatim in the source · abstract, characters 805–855 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Hypocotyl length decreased with increasing water stress in PEG 6000 solution.

Taxon
B. juncea var. EnsabiBrassica junceae1
Treatment
PEG 6000 solutione0
Outcome
hypocotyl lengths of seedlingse0
Direction
decreasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In PEG 6000 solution, the hypocotyl lengths of seedlings decreased with an increase in water stress”

✓ located verbatim in the source · abstract, characters 857–956 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Shoot elongation significantly decreased at 2-8 MPa PEG 6000.

Taxon
B. juncea var. EnsabiBrassica junceae1
Treatment
concentration of 2-8 MPae0
Outcome
Shoots elongatione0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Shoots elongation significantly decreased by concentration of 2-8 MPa”

✓ located verbatim in the source · abstract, characters 958–1027 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑
e2

“In PEG 6000 solution”

✓ located verbatim in the source · abstract, characters 857–877 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Hypocotyl elongation was absent at 10 and 12 MPa.

Taxon
B. juncea var. EnsabiBrassica junceae1
Treatment
concentration of 10 and 12 MPae0
Outcome
hypocotyl elongatione0
Direction
noe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“no hypocotyl elongation at concentration of 10 and 12 MPa”

✓ located verbatim in the source · abstract, characters 1036–1093 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Shoot elongation was completely inhibited at 10 and 12 MPa.

Taxon
B. juncea var. EnsabiBrassica junceae1
Treatment
concentration of 10 and 12 MPae0
Outcome
shoot elongatione0
Direction
completely inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica juncea
Brassica juncea · TaxonDalgial · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“no hypocotyl elongation at concentration of 10 and 12 MPa and shoot elongation completely inhibited”

✓ located verbatim in the source · abstract, characters 1036–1135 · show in text ↑
e1

“B. juncea var. Ensabi”

✓ located verbatim in the source · abstract, characters 280–301 · show in text ↑

drought · growth · Brassica juncea (Indian mustard)Positive and negative results

5 papers: 1 positive, 4 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.