Abstract
The response of the ascorbate-glutathione cycle was investigated in roots of young wheat (Triticum aestivum L.) seedlings that were deprived of oxygen either by subjecting them to root hypoxia or to entire plant anoxia and then re-aerated. Although higher total levels of ascorbate and glutathione were observed under hypoxia, only the total amount of ascorbate was increased under anoxia. Under both treatments a significant increase in the reduced form of ascorbate and glutathione was found, resulting in increased reduction states. Upon the onset of re-aeration the ratios started to decline rapidly, indicating oxidative stress. Hypoxia caused higher activity of ascorbate peroxidase, whereas activities of monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase were diminished or only slightly influenced. Under anoxia, activities of ascorbate peroxidase and glutathione reductase decreased significantly to 39 and 62%, respectively. However, after re-aeration of hypoxically or anoxically pretreated roots, activity of enzymes approached the control levels. This corresponds with the restoration of the high reduction state of ascorbate and glutathione within 16 to 96 h of re-aeration, depending on the previous duration of anoxia. Apparently, anoxia followed by re-aeration more severely impairs entire plant metabolism compared with hypoxia, thus leading to decreased viability.
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Claims 11
Claim · model-written summary
Hypoxia increased total ascorbate and glutathione in wheat roots.
- Taxon
- wheat
Triticum aestivume1 - Treatment
- hypoxiae0
- Outcome
- total levels of ascorbate and glutathionee0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher total levels of ascorbate and glutathione were observed under hypoxia”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
Anoxia increased total ascorbate in wheat roots.
- Taxon
- wheatTriticum aestivume1
- Treatment
- anoxiae0
- Outcome
- total amount of ascorbatee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only the total amount of ascorbate was increased under anoxia”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
Both hypoxia and anoxia significantly increased reduced ascorbate and glutathione and their reduction states in wheat roots.
- Taxon
- wheatTriticum aestivume2
- Treatment
- root hypoxia or to entire plant anoxiae1
- Outcome
- reduced form of ascorbate and glutathionee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under both treatments a significant increase in the reduced form of ascorbate and glutathione was found, resulting in increased reduction states.”
e1“root hypoxia or to entire plant anoxia”
e2“wheat (Triticum aestivum L.)”
Claim · model-written summary
Reduction state ratios rapidly declined after re aeration, indicating oxidative stress in wheat roots.
- Taxon
- wheatTriticum aestivume1
- Treatment
- re-aeratione0
- Outcome
- ratiose0
- Direction
- decline rapidlye0
- Qualifier
- indicating oxidative stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon the onset of re-aeration the ratios started to decline rapidly, indicating oxidative stress.”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
Hypoxia increased ascorbate peroxidase activity in wheat roots.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Hypoxiae0
- Gene
- ascorbate peroxidasee0
- Outcome
- activity of ascorbate peroxidasee0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypoxia caused higher activity of ascorbate peroxidase”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
Under hypoxia, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase activities were diminished or only slightly influenced.
- Taxon
- wheatTriticum aestivume2
- Treatment
- Hypoxiae1
- Outcome
- activities of monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductasee0
- Direction
- diminished or only slightly influencede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activities of monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase were diminished or only slightly influenced.”
e1“Hypoxia caused higher activity”
e2“wheat (Triticum aestivum L.)”
Claim · model-written summary
Anoxia significantly lowered wheat root ascorbate peroxidase activity to 39% of control.
- Taxon
- wheatTriticum aestivume1
- Treatment
- anoxiae0
- Gene
- ascorbate peroxidasee0
- Outcome
- activities of ascorbate peroxidasee0
- Direction
- decreasede0
- Value
- 39e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under anoxia, activities of ascorbate peroxidase and glutathione reductase decreased significantly to 39 and 62%, respectively.”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
Anoxia significantly lowered wheat root glutathione reductase activity to 62%.
- Taxon
- wheatTriticum aestivume1
- Treatment
- anoxiae0
- Gene
- glutathione reductasee0
- Outcome
- glutathione reductasee0
- Direction
- decreasede0
- Value
- 62%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under anoxia, activities of ascorbate peroxidase and glutathione reductase decreased significantly to 39 and 62%, respectively.”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
After re aeration, antioxidant enzyme activities approached control levels in previously hypoxic or anoxic wheat roots.
- Taxon
- wheatTriticum aestivume1
- Treatment
- re-aeratione0
- Outcome
- activity of enzymese0
- Direction
- approachede0
- Comparator
- control levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after re-aeration of hypoxically or anoxically pretreated roots, activity of enzymes approached the control levels.”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
High ascorbate and glutathione reduction states were restored within 16 to 96 h after re aeration.
- Taxon
- wheatTriticum aestivume1
- Outcome
- high reduction state of ascorbate and glutathionee0
- Value
- 16 to 96 he0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“restoration of the high reduction state of ascorbate and glutathione within 16 to 96 h of re-aeration”
e1“wheat (Triticum aestivum L.)”
Claim · model-written summary
Anoxia followed by re aeration impaired wheat metabolism more severely than hypoxia and reduced viability.
- Taxon
- wheatTriticum aestivume1
- Treatment
- anoxia followed by re-aeratione0
- Outcome
- entire plant metabolisme0
- Direction
- more severely impairse0
- Qualifier
- decreased viabilitye0
- Comparator
- hypoxiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anoxia followed by re-aeration more severely impairs entire plant metabolism compared with hypoxia, thus leading to decreased viability.”
e1“wheat (Triticum aestivum L.)”
The same questions in other papers
flooding · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
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- negative
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29 papers more in this group
See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.