Abstract
The mutualistic symbiont Piriformospora indica exhibits a great potential in agriculture. The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development. The resulting highly bushy root phenotype of colonized Chinese cabbage seedlings differs substantially from reports of other plant species, which prompted the more detailed study of this symbiosis. A large-scale expressed sequence tag (EST) data set was obtained from a double-subtractive EST library, by subtracting the cDNAs of Chinese cabbage root tissue and of P. indica mycelium from those of P. indica-colonized root tissue. The analysis revealed ~700 unique genes rooted in 141 clusters and 559 singles. A total of 66% of the sequences could be annotated in the NCBI GenBank. Genes which are stimulated by P. indica are involved in various types of transport, carbohydrate metabolism, auxin signalling, cell wall metabolism, and root development, including the root hair-forming phosphoinositide phosphatase 4. For 20 key genes, induction by fungal colonization was confirmed kinetically during the interaction by real-time reverse transcription-PCR. Moreover, the auxin concentration increases transiently after exposure of the roots to P. indica. Microscopic analyses demonstrated that the development of the root maturation zone is the major target of P. indica in Chinese cabbage. Taken together, the symbiotic interaction between Chinese cabbage and P. indica is a novel model to study root growth promotion which, in turn, is important for agriculture and plant biotechnology.
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Claims 8
Claim · model-written summary
Piriformospora indica interaction promotes growth and biomass of Chinese cabbage
- Taxon
- Chinese cabbage (Brassica campestris cv. Chinensis)
Brassica rapa subsp. pekinensise1 - Treatment
- interaction between P. indica and Chinese cabbagee1
- Interactor
- P. indicaSerendipita indicae1symbiont
- Outcome
- growth and biomasse1
- Direction
- promotione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development”
Claim · model-written summary
Piriformospora indica colonization increases root hair development in Chinese cabbage
- Taxon
- Chinese cabbage (Brassica campestris cv. Chinensis)Brassica rapa subsp. pekinensise1
- Treatment
- interaction between P. indica and Chinese cabbagee1
- Interactor
- P. indicaSerendipita indicae1symbiont
- Outcome
- root hair developmente1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development”
Claim · model-written summary
The EST data set analysis revealed about 700 unique genes in Chinese cabbage roots colonized by P. indica
- Taxon
- Chinese cabbage (Brassica campestris cv. Chinensis)Brassica rapa subsp. pekinensise1
- Outcome
- unique genese2
- Value
- ~700e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development”
e2“The analysis revealed ~700 unique genes rooted in 141 clusters and 559 singles”
Claim · model-written summary
About 66% of the EST sequences from Chinese cabbage roots could be annotated in NCBI GenBank
- Taxon
- Chinese cabbage (Brassica campestris cv. Chinensis)Brassica rapa subsp. pekinensise1
- Outcome
- sequences could be annotatede3
- Value
- 66%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development”
e3“A total of 66% of the sequences could be annotated in the NCBI GenBank”
Claim · model-written summary
Genes stimulated by P. indica in Chinese cabbage include the root hair-forming phosphoinositide phosphatase 4
- Taxon
- Chinese cabbage (Brassica campestris cv. Chinensis)Brassica rapa subsp. pekinensise1
- Treatment
- P. indicae4
- Interactor
- P. indicaSerendipita indicae4symbiont
- Gene
- root hair-forming phosphoinositide phosphatase 4e4
- Outcome
- Genese4
- Direction
- stimulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development”
e4“Genes which are stimulated by P. indica are involved in various types of transport, carbohydrate metabolism, auxin signalling, cell wall metabolism, and root development, including the root hair-forming phosphoinositide phosphatase 4”
Claim · model-written summary
Twenty key genes in Chinese cabbage roots showed induction by fungal colonization with P. indica
- Taxon
- Chinese cabbage (Brassica campestris cv. Chinensis)Brassica rapa subsp. pekinensise1
- Treatment
- fungal colonizatione5
- Interactor
- P. indicaSerendipita indicae1symbiont
- Outcome
- 20 key genese5
- Direction
- inductione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development”
e5“For 20 key genes, induction by fungal colonization was confirmed kinetically during the interaction by real-time reverse transcription-PCR”
Claim · model-written summary
Piriformospora indica exposure transiently increased auxin concentration in Chinese cabbage roots
- Taxon
- Chinese cabbage (Brassica campestris cv. Chinensis)Brassica rapa subsp. pekinensise1
- Treatment
- exposure of the roots to P. indicae6
- Interactor
- P. indicaSerendipita indicae6symbiont
- Outcome
- auxin concentratione6
- Direction
- increasese6
- Qualifier
- transientlye6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development”
e6“Moreover, the auxin concentration increases transiently after exposure of the roots to P. indica”
Claim · model-written summary
Root maturation zone development is the major target of Piriformospora indica in Chinese cabbage
- Taxon
- Chinese cabbageBrassica rapa subsp. pekinensise7
- Organ / stage
- root maturation zonee7
- Interactor
- P. indicaSerendipita indicae7symbiont
- Outcome
- development of the root maturation zonee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The interaction between P. indica and Chinese cabbage (Brassica campestris cv. Chinensis) results in growth and biomass promotion of the host plant and in particular in root hair development”
e7“Microscopic analyses demonstrated that the development of the root maturation zone is the major target of P. indica in Chinese cabbage”
The same questions in other papers
Brassica rapa subsp. pekinensis · growth · Brassica rapa (field mustard)Positive and negative results
1 in the opposite direction
- negative
Serendipita indica · growth · Brassica rapa (field mustard)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.