Abstract
Transgenic potato ( Solanum tuberosum L.) lines of two cultivars, Ilam Hardy and Iwa, were developed using Agrobacterium -mediated transformation to transfer a cry 1Ac9 gene under the transcriptional control of the CaMV 35S promoter. PCR confirmed the presence of the npt II selectable marker gene in all recovered lines. All ten lines of Ilam Hardy and 14 of 15 Iwa lines were PCR-positive for the cry gene. In greenhouse trials, all Ilam Hardy transgenic lines produced phenotypically normal plants and significantly inhibited larval growth of potato tuber moth ( Phthorimaea operculella Zeller). In contrast, only 60% of the Iwa transgenic lines produced phenotypically normal plants, but all lines positive for the cry gene significantly inhibited larval growth. All transgenic lines with a greenhouse appearance equivalent to the nontransgenic controls and improved resistance to potato tuber moth larvae were planted in the field. Three of the ten Ilam Hardy lines and two of the eight Iwa lines retained phenotypically normal appearance in the field and produced tuber yields equivalent to the nontransgenic controls. All five of these transgenic lines significantly inhibited larval growth of potato tuber moth on excised field-grown leaves. A high correlation was established between larval growth indices from the greenhouse and the field. A transgenic line from each cultivar inhibited larval growth by over 40%, and the line derived from Ilam Hardy prevented pupation of all larvae. Southern analysis on these five elite lines revealed that they contained either one or two copies of the cry 1Ac9 gene. The amount of Cry protein in all transgenic lines tested was less than 60 ng·g -1 of fresh leaf tissue. A transgenic line from each cultivar was identified with comparable phenotypic appearance and yield to their parent cultivars coupled with high resistance to PTM.
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Claims 12
Claim · model-written summary
PCR found the npt II marker in all recovered lines.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- npt IIe0
- Outcome
- presence of the npt II selectable marker genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR confirmed the presence of the npt II selectable marker gene in all recovered lines”
t0“Potatoes Transgenic”
Claim · model-written summary
All ten Ilam Hardy lines were PCR positive for the cry gene.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Ilam Hardye0
- Gene
- cry genee0
- Outcome
- PCR-positive for the cry genee0
- Value
- All ten linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All ten lines of Ilam Hardy and 14 of 15 Iwa lines were PCR-positive for the cry gene”
t0“Potatoes Transgenic”
Claim · model-written summary
Fourteen of fifteen Iwa lines were PCR positive for the cry gene.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Iwae0
- Gene
- cry genee0
- Outcome
- PCR-positive for the cry genee0
- Value
- 14 of 15e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All ten lines of Ilam Hardy and 14 of 15 Iwa lines were PCR-positive for the cry gene”
t0“Potatoes Transgenic”
Claim · model-written summary
All Ilam Hardy transgenic lines produced phenotypically normal greenhouse plants.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Ilam Hardye0
- Outcome
- phenotypically normal plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all Ilam Hardy transgenic lines produced phenotypically normal plants”
t0“Potatoes Transgenic”
Claim · model-written summary
Ilam Hardy transgenic lines significantly inhibited tuber moth larval growth in greenhouse trials.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Ilam Hardye0
- Treatment
- transgenic linese0
- Interactor
- potato tuber mothe0pest
- Outcome
- larval growth of potato tuber mothe0
- Direction
- inhibitede0
- Setting
- greenhouse trialse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In greenhouse trials, all Ilam Hardy transgenic lines produced phenotypically normal plants and significantly inhibited larval growth of potato tuber moth ( Phthorimaea operculella Zeller)”
t0“Potatoes Transgenic”
Claim · model-written summary
Only 60% of Iwa transgenic lines produced normal plants.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Iwae0
- Outcome
- phenotypically normal plantse0
- Value
- 60%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only 60% of the Iwa transgenic lines produced phenotypically normal plants”
t0“Potatoes Transgenic”
Claim · model-written summary
Three of ten Ilam Hardy lines retained normal field appearance and yields equivalent to controls.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Ilam Hardye0
- Outcome
- phenotypically normal appearance in the field and produced tuber yields equivalente0
- Value
- Three of the tene0
- Comparator
- nontransgenic controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three of the ten Ilam Hardy lines and two of the eight Iwa lines retained phenotypically normal appearance in the field and produced tuber yields equivalent to the nontransgenic controls”
t0“Potatoes Transgenic”
Claim · model-written summary
Two of eight Iwa lines retained normal field appearance and yields equivalent to controls.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Iwae0
- Outcome
- phenotypically normal appearance in the field and produced tuber yields equivalente0
- Value
- two of the eighte0
- Comparator
- nontransgenic controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three of the ten Ilam Hardy lines and two of the eight Iwa lines retained phenotypically normal appearance in the field and produced tuber yields equivalent to the nontransgenic controls”
t0“Potatoes Transgenic”
Claim · model-written summary
All five elite lines significantly inhibited moth larval growth on excised field leaves.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- field-grown leavese0
- Treatment
- transgenic linese0
- Interactor
- potato tuber mothe0pest
- Outcome
- larval growth of potato tuber mothe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All five of these transgenic lines significantly inhibited larval growth of potato tuber moth on excised field-grown leaves”
t0“Potatoes Transgenic”
Claim · model-written summary
Greenhouse and field larval growth indices were highly correlated.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- larval growth indicese0
- Setting
- greenhouse and the fielde0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A high correlation was established between larval growth indices from the greenhouse and the field”
t0“Potatoes Transgenic”
Claim · model-written summary
Five elite lines had one or two cry1Ac9 gene copies.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- cry 1Ac9 genee0
- Outcome
- one or two copies of the cry 1Ac9 genee0
- Value
- one or two copiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they contained either one or two copies of the cry 1Ac9 gene”
t0“Potatoes Transgenic”
Claim · model-written summary
Cry protein was below 60 ng per gram fresh leaf tissue in tested lines.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- fresh leaf tissuee0
- Gene
- Cry proteine0
- Outcome
- amount of Cry proteine0
- Direction
- less thane0
- Value
- 60 ng·g -1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amount of Cry protein in all transgenic lines tested was less than 60 ng·g -1 of fresh leaf tissue”
t0“Potatoes Transgenic”
The same questions in other papers
transgenic · growth · Solanum tuberosum (potato)Positive and negative results
9 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- no effect
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.