Abstract
Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants. To quantify the inhibition, activity was compared in corresponding leaves down a plant and in plants of different ages. Aldolase activity was decreased by 32-43%, 56-71%, 79-83% and 91-97% in A-70, A-3, A-51 and A-2. Separation on a Q-Sepharose-FF column showed the decrease was due to inhibition of plastid aldolase. The transformants showed a small increase of Rubisco activity, a small decrease of phosphoribulokinase activity, and larger but subproportional decreases of sedoheptulose-1,7-biphosphatase and plastid fructose-1,6-bisphosphatase activity. Ambient photosynthesis was inhibited by 10%, 40%, 66% and 85% in A-70, A-3, A-51 and A-2. The transformants contained increased triose phosphates, and very low ribulose-1,5-bisphosphate and glycerate-3-phosphate. Chlorophyll fluorescence indicated that photosystem II was more reduced and thylakoid energization was increased. Starch synthesis was decreased by 16% and 36% in A-70 and A-3, whereas sucrose synthesis was less strongly inhibited. Plant growth was not significantly altered in A-70, was decreased by 41% in A-3, and was severely inhibited in plants with under 20% of wild-type aldolase activity. Although plastid aldolase catalyses a readily reversible reaction, possesses no known regulatory properties, and would appear irrelevant for the control of metabolism and growth, small changes in its activity have marked consequences for photosynthesis, carbon partitioning and growth.
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Claims 20
Claim · model-written summary
Antisense expression of plastid aldolase decreased aldolase expression at the transcript and protein level in several potato transformants.
- Taxon
- potato
Solanum tuberosume0 - Material
- 'antisense' potato transformantse0
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Gene
- plastid aldolasee0
- Outcome
- expression of aldolase at the transcript and protein levele0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
Claim · model-written summary
Aldolase activity was decreased by 32-43% in transformant A-70.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-70e1
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Gene
- plastid aldolasee0
- Outcome
- Aldolase activitye1
- Direction
- decreasede1
- Value
- 32-43%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e1“Aldolase activity was decreased by 32-43%, 56-71%, 79-83% and 91-97% in A-70, A-3, A-51 and A-2”
Claim · model-written summary
Aldolase activity was decreased by 56-71% in transformant A-3.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-3e1
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Gene
- plastid aldolasee0
- Outcome
- Aldolase activitye1
- Direction
- decreasede1
- Value
- 56-71%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e1“Aldolase activity was decreased by 32-43%, 56-71%, 79-83% and 91-97% in A-70, A-3, A-51 and A-2”
Claim · model-written summary
Aldolase activity was decreased by 79-83% in transformant A-51.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-51e1
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Gene
- plastid aldolasee0
- Outcome
- Aldolase activitye1
- Direction
- decreasede1
- Value
- 79-83%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e1“Aldolase activity was decreased by 32-43%, 56-71%, 79-83% and 91-97% in A-70, A-3, A-51 and A-2”
Claim · model-written summary
Aldolase activity was decreased by 91-97% in transformant A-2.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-2e1
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Gene
- plastid aldolasee0
- Outcome
- Aldolase activitye1
- Direction
- decreasede1
- Value
- 91-97%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e1“Aldolase activity was decreased by 32-43%, 56-71%, 79-83% and 91-97% in A-70, A-3, A-51 and A-2”
Claim · model-written summary
Q-Sepharose separation showed that the decrease in aldolase activity was due to inhibition of plastid aldolase.
- Taxon
- potatoSolanum tuberosume0
- Gene
- plastid aldolasee2
- Outcome
- decreasee2
- Qualifier
- due to inhibition of plastid aldolasee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e2“Separation on a Q-Sepharose-FF column showed the decrease was due to inhibition of plastid aldolase”
Claim · model-written summary
The transformants showed a small increase in Rubisco activity.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Rubisco activitye3
- Direction
- increasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e3“The transformants showed a small increase of Rubisco activity”
Claim · model-written summary
The transformants showed a small decrease in phosphoribulokinase activity and larger but subproportional decreases in sedoheptulose-1,7-biphosphatase and plastid fructose-1,6-bisphosphatase activity.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Gene
- sedoheptulose-1,7-biphosphatase and plastid fructose-1,6-bisphosphatasee4
- Outcome
- phosphoribulokinase activitye4
- Direction
- decreasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e4“a small decrease of phosphoribulokinase activity, and larger but subproportional decreases of sedoheptulose-1,7-biphosphatase and plastid fructose-1,6-bisphosphatase activity”
Claim · model-written summary
The transformants contained increased triose phosphates.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- triose phosphatese5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e5“The transformants contained increased triose phosphates”
Claim · model-written summary
The transformants contained very low ribulose-1,5-bisphosphate and glycerate-3-phosphate.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- ribulose-1,5-bisphosphate and glycerate-3-phosphatee6
- Direction
- very lowe6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e6“very low ribulose-1,5-bisphosphate and glycerate-3-phosphate”
Claim · model-written summary
Thylakoid energization was increased in the transformants, with photosystem II more reduced.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- thylakoid energizatione7
- Direction
- increasede7
- Qualifier
- photosystem II was more reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e7“Chlorophyll fluorescence indicated that photosystem II was more reduced and thylakoid energization was increased”
Claim · model-written summary
Starch synthesis was decreased by 16% in transformant A-70.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-70e4
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Starch synthesise4
- Direction
- decreasede4
- Value
- 16%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e4“Starch synthesis was decreased by 16% and 36% in A-70 and A-3”
Claim · model-written summary
Starch synthesis was decreased by 36% in transformant A-3.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-3e4
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Starch synthesise4
- Direction
- decreasede4
- Value
- 36%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e4“Starch synthesis was decreased by 16% and 36% in A-70 and A-3”
Claim · model-written summary
Sucrose synthesis was less strongly inhibited than starch synthesis in the transformants.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- sucrose synthesise5
- Direction
- less strongly inhibitede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e4“Starch synthesis was decreased by 16% and 36% in A-70 and A-3”
e5“whereas sucrose synthesis was less strongly inhibited”
Claim · model-written summary
Ambient photosynthesis was inhibited by 10% in transformant A-70.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-70e6
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Ambient photosynthesise6
- Direction
- inhibitede6
- Value
- 10%e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e6“Ambient photosynthesis was inhibited by 10%, 40%, 66% and 85% in A-70, A-3, A-51 and A-2”
Claim · model-written summary
Ambient photosynthesis was inhibited by 40% in transformant A-3.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-3e6
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Ambient photosynthesise6
- Direction
- inhibitede6
- Value
- 40%e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e6“Ambient photosynthesis was inhibited by 10%, 40%, 66% and 85% in A-70, A-3, A-51 and A-2”
Claim · model-written summary
Ambient photosynthesis was inhibited by 66% in transformant A-51.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-51e6
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Ambient photosynthesise6
- Direction
- inhibitede6
- Value
- 66%e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e6“Ambient photosynthesis was inhibited by 10%, 40%, 66% and 85% in A-70, A-3, A-51 and A-2”
Claim · model-written summary
Ambient photosynthesis was inhibited by 85% in transformant A-2.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-2e6
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Ambient photosynthesise6
- Direction
- inhibitede6
- Value
- 85%e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e6“Ambient photosynthesis was inhibited by 10%, 40%, 66% and 85% in A-70, A-3, A-51 and A-2”
Claim · model-written summary
Plant growth was not significantly altered in transformant A-70.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-70e7
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Plant growthe7
- Direction
- not significantly alterede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e7“Plant growth was not significantly altered in A-70, was decreased by 41% in A-3, and was severely inhibited in plants with under 20% of wild-type aldolase activity”
Claim · model-written summary
Plant growth was decreased by 41% in transformant A-3 and was severely inhibited in plants with under 20% of wild-type aldolase activity.
- Taxon
- potatoSolanum tuberosume0
- Material
- A-3e7
- Treatment
- Antisense expression of a full length cDNA encoding plastid aldolasee0
- Outcome
- Plant growthe7
- Direction
- decreasede7
- Value
- 41%e7
- Qualifier
- severely inhibited in plants with under 20% of wild-type aldolase activitye7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e7“Plant growth was not significantly altered in A-70, was decreased by 41% in A-3, and was severely inhibited in plants with under 20% of wild-type aldolase activity”
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