Abstract
A rapid decrease of the plant hormone ABA under submergence is thought to be a prerequisite for the enhanced elongation of submerged shoots of rice (Oryza sativa L.). Here, we report that the level of phaseic acid (PA), an oxidized form of ABA, increased with decreasing ABA level during submergence. The oxidation of ABA to PA is catalyzed by ABA 8'-hydroxylase, which is possibly encoded by three genes (OsABA8ox1, -2 and -3) in rice. The ABA 8'-hydroxylase activity was confirmed in microsomes from yeast expressing OsABA8ox1. OsABA8ox1-green fluorescent protein (GFP) fusion protein in onion cells was localized to the endoplasmic reticulum. The mRNA level of OsABA8ox1, but not the mRNA levels of other OsABA8ox genes, increased dramatically within 1 h after submergence. On the other hand, the mRNA levels of genes involved in ABA biosynthesis (OsZEP and OsNCEDs) decreased after 1-2 h of submergence. Treatment of aerobic seedlings with ethylene and its precursor, 1-aminocyclopropane-1-carboxylate (ACC), rapidly induced the expression of OsABA8ox1, but the ethylene treatment did not strongly affect the expression of ABA biosynthetic genes. Moreover, pre-treatment with 1-methylcyclopropene (1-MCP), a potent inhibitor of ethylene action, partially suppressed induction of OsABA8ox1 expression under submergence. The ABA level was found to be negatively correlated with OsABA8ox1 expression under ACC or 1-MCP treatment. Together, these results indicate that the rapid decrease in ABA levels in submerged rice shoots is controlled partly by ethylene-induced expression of OsABA8ox1 and partly by ethylene-independent suppression of genes involved in the biosynthesis of ABA.
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Claims 10
Claim · model-written summary
Phaseic acid level increased as ABA level decreased during submergence in rice.
- Taxon
- rice
Oryza sativae1 - Treatment
- submergencee0
- Compound
- ABAe0
- Outcome
- level of phaseic acid (PA)e0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e0“the level of phaseic acid (PA), an oxidized form of ABA, increased with decreasing ABA level during submergence”
Claim · model-written summary
ABA level was negatively correlated with OsABA8ox1 expression under ACC or 1-MCP treatment in rice.
- Taxon
- riceOryza sativae1
- Treatment
- ACC or 1-MCP treatmente0
- Gene
- OsABA8ox1e0
- Compound
- ABAe0
- Outcome
- ABA levele0
- Direction
- decreaseinferrede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e0“The ABA level was found to be negatively correlated with OsABA8ox1 expression under ACC or 1-MCP treatment”
Claim · model-written summary
Ethylene and its precursor ACC rapidly induced OsABA8ox1 expression in aerobic rice seedlings.
- Taxon
- riceOryza sativae1
- Organ / stage
- aerobic seedlingse2
- Treatment
- ethylene and its precursor, 1-aminocyclopropane-1-carboxylate (ACC)e2
- Gene
- OsABA8ox1e2
- Outcome
- expression of OsABA8ox1e2
- Direction
- inducede2
- Qualifier
- rapidlye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e2“Treatment of aerobic seedlings with ethylene and its precursor, 1-aminocyclopropane-1-carboxylate (ACC), rapidly induced the expression of OsABA8ox1, but the ethylene treatment did not strongly affect the expression of ABA biosynthetic genes”
Claim · model-written summary
Ethylene treatment did not strongly affect the expression of ABA biosynthetic genes in rice.
- Taxon
- riceOryza sativae1
- Treatment
- ethylene treatmente2
- Outcome
- expression of ABA biosynthetic genese2
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e2“Treatment of aerobic seedlings with ethylene and its precursor, 1-aminocyclopropane-1-carboxylate (ACC), rapidly induced the expression of OsABA8ox1, but the ethylene treatment did not strongly affect the expression of ABA biosynthetic genes”
Claim · model-written summary
OsABA8ox1 mRNA increased dramatically within 1 h after submergence, unlike other OsABA8ox genes in rice.
- Taxon
- riceOryza sativae1
- Treatment
- submergencee3
- Gene
- OsABA8ox1e3
- Outcome
- mRNA level of OsABA8ox1e3
- Direction
- increasede3
- Qualifier
- dramatically within 1 he3
- Comparator
- other OsABA8ox genese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e3“The mRNA level of OsABA8ox1, but not the mRNA levels of other OsABA8ox genes, increased dramatically within 1 h after submergence”
Claim · model-written summary
Submergence decreased mRNA levels of ABA biosynthesis genes (OsZEP and OsNCEDs) after 1-2 h in rice.
- Taxon
- riceOryza sativae1
- Treatment
- submergencee4
- Gene
- OsZEP and OsNCEDse4
- Outcome
- mRNA levels of genes involved in ABA biosynthesise4
- Direction
- decreasede4
- Qualifier
- after 1-2 he4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e4“On the other hand, the mRNA levels of genes involved in ABA biosynthesis (OsZEP and OsNCEDs) decreased after 1-2 h of submergence”
Claim · model-written summary
Pre-treatment with 1-MCP partially suppressed the induction of OsABA8ox1 expression under submergence in rice.
- Taxon
- riceOryza sativae1
- Treatment
- pre-treatment with 1-methylcyclopropene (1-MCP)e5
- Gene
- OsABA8ox1e5
- Outcome
- induction of OsABA8ox1 expressione5
- Direction
- suppressede5
- Qualifier
- under submergencee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e5“Moreover, pre-treatment with 1-methylcyclopropene (1-MCP), a potent inhibitor of ethylene action, partially suppressed induction of OsABA8ox1 expression under submergence”
Claim · model-written summary
The rapid ABA decrease in submerged rice shoots is controlled partly by ethylene-induced OsABA8ox1 expression and partly by ethylene-independent suppression of ABA biosynthesis genes.
- Taxon
- riceOryza sativae0
- Gene
- OsABA8ox1e0
- Outcome
- decrease in ABA levelse0
- Qualifier
- ethylene-independent suppression of genes involved in the biosynthesis of ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Together, these results indicate that the rapid decrease in ABA levels in submerged rice shoots is controlled partly by ethylene-induced expression of OsABA8ox1 and partly by ethylene-independent suppression of genes involved in the biosynthesis of ABA”
Claim · model-written summary
The ABA 8'-hydroxylase activity of OsABA8ox1 was confirmed in microsomes from yeast expressing it.
- Taxon
- riceOryza sativae1
- Gene
- OsABA8ox1e2
- Outcome
- ABA 8'-hydroxylase activitye2
- Qualifier
- microsomes from yeast expressing OsABA8ox1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e2“The ABA 8'-hydroxylase activity was confirmed in microsomes from yeast expressing OsABA8ox1”
Claim · model-written summary
The OsABA8ox1-GFP fusion protein was localized to the endoplasmic reticulum in onion cells.
- Taxon
- riceOryza sativae1
- Organ / stage
- onion cellse3
- Gene
- OsABA8ox1e3
- Outcome
- localized to the endoplasmic reticulume3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“enhanced elongation of submerged shoots of rice (Oryza sativa L.)”
e3“OsABA8ox1-green fluorescent protein (GFP) fusion protein in onion cells was localized to the endoplasmic reticulum”
The same questions in other papers
ethene · molecular: expression · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 6 in the opposite direction
- positive
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