Abstract
A putative citrus vacuolar processing thiolprotease cDNA (Cit-vac) was isolated from a cDNA library of Citrus fruits (Citrus sinensis L. Osbeck var Washington navel). The cDNA is 58 and 57% identical with vacuolar processing seed proteases from castor bean and soybean, respectively. The Citvac sequence shows a typical signal peptide for entering into the endoplasmic reticulum and two glycosylation signals. Using an in vitro transcription-translation system, we show that the Citvac precursor is able to enter a microsomal fraction and to undergo proteolytic processing and glycosylation. Transcript levels for the Citvac are developmentally regulated in the flavedo (outer colored part of the fruit peel) and increase during fruit ripening and in the flower during opening. Exogenous treatment with ethylene induces Citvac mRNA expression in both fruits and leaves. Citvac is encoded by one or two genes in the Citrus genome. The possible role of the Citvac gene product during fruit ripening and other ethylene-mediated processes is discussed.
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Claims 9
Claim · model-written summary
Cit-vac cDNA was isolated from Citrus fruit.
Verbatim
e0“A putative citrus vacuolar processing thiolprotease cDNA (Cit-vac) was isolated from a cDNA library of Citrus fruits (Citrus sinensis L. Osbeck var Washington navel).”
Claim · model-written summary
Cit-vac is 58% identical to castor bean seed proteases.
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cDNA is 58 and 57% identical with vacuolar processing seed proteases from castor bean and soybean, respectively.”
e1“A putative citrus vacuolar processing thiolprotease cDNA”
Claim · model-written summary
Cit-vac is 57% identical to soybean seed proteases.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cDNA is 58 and 57% identical with vacuolar processing seed proteases from castor bean and soybean, respectively.”
e1“A putative citrus vacuolar processing thiolprotease cDNA”
Claim · model-written summary
Citvac sequence has a signal peptide and two glycosylation signals.
- Gene
- Citvace0
- Outcome
- a typical signal peptide for entering into the endoplasmic reticulum and two glycosylation signalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Citvac sequence shows a typical signal peptide for entering into the endoplasmic reticulum and two glycosylation signals.”
e1“Citrus genome”
Claim · model-written summary
Citvac precursor enters microsomes and undergoes processing and glycosylation in vitro.
- Gene
- Citvace0
- Outcome
- enter a microsomal fraction and to undergo proteolytic processing and glycosylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Citvac precursor is able to enter a microsomal fraction and to undergo proteolytic processing and glycosylation.”
e1“Citrus genome”
Claim · model-written summary
Citvac transcript increases in flavedo during fruit ripening.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels for the Citvac are developmentally regulated in the flavedo (outer colored part of the fruit peel) and increase during fruit ripening”
e1“Citrus genome”
Claim · model-written summary
Citvac transcript increases in flowers during opening.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and in the flower during opening.”
e1“Exogenous treatment with ethylene induces Citvac mRNA expression in both fruits and leaves. Citvac is encoded by one or two genes in the Citrus genome.”
Claim · model-written summary
Ethylene induces Citvac mRNA in fruit and leaves.
- Organ / stage
- fruits and leavese0
- Treatment
- Exogenous treatment with ethylenee0
- Outcome
- Citvac mRNA expressione0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous treatment with ethylene induces Citvac mRNA expression in both fruits and leaves.”
e1“Citrus genome”
Claim · model-written summary
Citvac is encoded by one or two genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Citvac is encoded by one or two genes in the Citrus genome.”
The same questions in other papers
ethene · molecular: expression · CitrusPositive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.