Abstract
The tomato Pti4 gene encodes a transcription factor that was identified on the basis of its specific interaction with the product of the Pto disease resistance gene in a yeast two-hybrid system. We show here that the Pti4 protein specifically binds the GCC-box cis element, which is present in the promoter region of many pathogenesis-related (PR) genes. Expression of the Pti4 gene in tomato leaves was rapidly induced by ethylene and by infection with Pseudomonas syringae pv tomato, and this induction preceded expression of GCC-box-containing PR genes. Although salicylic acid also induced Pti4 gene expression, it did not induce GCC-box PR genes. Rather, salicylic acid antagonized ethylene-mediated expression of GCC-box PR genes. We demonstrate that the Pti4 protein is specifically phosphorylated by the Pto kinase and that this phosphorylation enhances binding of Pti4 to the GCC box. In addition, induced overexpression of Pto and Pti4 in tomato leaves resulted in a concomitant increase in GCC-box PR genes. Our results support a model in which phosphorylation of the Pti4 protein by the Pto kinase enhances the ability of Pti4 to activate expression of GCC-box PR genes in tomato.
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Claims 12
Claim · model-written summary
Pti4 encodes a transcription factor interacting with the Pto product.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- Pti4e0
- Outcome
- specific interaction with the product of the Pto disease resistance genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tomato Pti4 gene encodes a transcription factor that was identified on the basis of its specific interaction with the product of the Pto disease resistance gene in a yeast two-hybrid system.”
Claim · model-written summary
Pti4 protein specifically binds the GCC-box cis element.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Pti4e0
- Outcome
- binds the GCC-box cis elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Pti4 protein specifically binds the GCC-box cis element”
t0“Pti4 Is Induced by Ethylene and Salicylic Acid, and Its Product Is Phosphorylated by the Pto Kinase”
e1“The tomato Pti4 gene encodes a transcription factor”
Claim · model-written summary
Ethylene rapidly induced Pti4 expression in tomato leaves.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- leavese0
- Treatment
- ethylenee0
- Gene
- Pti4e0
- Outcome
- Expression of the Pti4 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the Pti4 gene in tomato leaves was rapidly induced by ethylene”
Claim · model-written summary
Pseudomonas syringae pv tomato infection rapidly induced Pti4 expression in tomato leaves.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- infection with Pseudomonas syringae pv tomatoe0
- Interactor
- Pseudomonas syringae pv tomato
Pseudomonas syringaee0pathogen - Gene
- Pti4e0
- Outcome
- Expression of the Pti4 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the Pti4 gene in tomato leaves was rapidly induced by ethylene and by infection with Pseudomonas syringae pv tomato”
Claim · model-written summary
Pti4 induction preceded expression of GCC-box-containing PR genes.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- GCC-box-containing PR genese0
- Outcome
- induction preceded expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this induction preceded expression of GCC-box-containing PR genes”
t0“Pti4 Is Induced by Ethylene and Salicylic Acid, and Its Product Is Phosphorylated by the Pto Kinase”
e1“The tomato Pti4 gene encodes a transcription factor”
Claim · model-written summary
Salicylic acid induced Pti4 gene expression.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- salicylic acide0
- Gene
- Pti4e0
- Outcome
- Pti4 gene expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salicylic acid also induced Pti4 gene expression”
t0“Pti4 Is Induced by Ethylene and Salicylic Acid, and Its Product Is Phosphorylated by the Pto Kinase”
e1“The tomato Pti4 gene encodes a transcription factor”
Claim · model-written summary
Salicylic acid did not induce GCC-box PR genes.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Salicylic Acidt0
- Gene
- GCC-box PR genese0
- Outcome
- GCC-box PR genese0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it did not induce GCC-box PR genes”
t0“Pti4 Is Induced by Ethylene and Salicylic Acid, and Its Product Is Phosphorylated by the Pto Kinase”
e1“The tomato Pti4 gene encodes a transcription factor”
Claim · model-written summary
Salicylic acid antagonized ethylene mediated GCC-box PR gene expression.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- salicylic acide0
- Gene
- GCC-box PR genese0
- Outcome
- expression of GCC-box PR genese0
- Direction
- antagonizede0
- Comparator
- ethylene-mediated expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salicylic acid antagonized ethylene-mediated expression of GCC-box PR genes”
t0“Pti4 Is Induced by Ethylene and Salicylic Acid, and Its Product Is Phosphorylated by the Pto Kinase”
e1“The tomato Pti4 gene encodes a transcription factor”
Claim · model-written summary
Pto kinase specifically phosphorylated Pti4 protein.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Pti4e0
- Outcome
- specifically phosphorylated by the Pto kinasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Pti4 protein is specifically phosphorylated by the Pto kinase”
t0“Pti4 Is Induced by Ethylene and Salicylic Acid, and Its Product Is Phosphorylated by the Pto Kinase”
e1“The tomato Pti4 gene encodes a transcription factor”
Claim · model-written summary
Pti4 phosphorylation enhanced its binding to the GCC box.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Pti4e0
- Outcome
- binding of Pti4 to the GCC boxe0
- Direction
- enhancese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this phosphorylation enhances binding of Pti4 to the GCC box”
t0“Pti4 Is Induced by Ethylene and Salicylic Acid, and Its Product Is Phosphorylated by the Pto Kinase”
e1“The tomato Pti4 gene encodes a transcription factor”
Claim · model-written summary
Induced Pto and Pti4 overexpression in tomato leaves increased GCC-box PR genes.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- leavese0
- Treatment
- induced overexpression of Pto and Pti4e0
- Gene
- Pto and Pti4e0
- Outcome
- GCC-box PR genese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced overexpression of Pto and Pti4 in tomato leaves resulted in a concomitant increase in GCC-box PR genes”
Claim · model-written summary
The authors propose that Pto phosphorylation enhances Pti4 activation of GCC-box PR genes.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- Pti4e0
- Outcome
- activate expression of GCC-box PR genese0
- Direction
- enhancese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phosphorylation of the Pti4 protein by the Pto kinase enhances the ability of Pti4 to activate expression of GCC-box PR genes in tomato”
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