Abstract
Root-specific promoters are valuable tools for targeting transgene expression, but many of those already described have limitations to their general applicability. We present the expression characteristics of SlREO, a novel gene isolated from tomato (Solanum lycopersicum L.). This gene was highly expressed in roots but had a very low level of expression in aerial plant organs. A 2.4-kb region representing the SlREO promoter sequence was cloned upstream of the uidA GUS reporter gene and shown to direct expression in the root cortex. In mature, glasshouse-grown plants this strict root specificity was maintained. Furthermore, promoter activity was unaffected by dehydration or wounding stress but was somewhat suppressed by exposure to NaCl, salicylic acid and jasmonic acid. The predicted protein sequence of SlREO contains a domain found in enzymes of the 2-oxoglutarate and Fe(II)-dependent dioxygenase superfamily. The novel SlREO promoter has properties ideal for applications requiring strong and specific gene expression in the bulk of tomato root tissue growing in soil, and is also likely to be useful in other Solanaceous crops.
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Claims 10
Claim · model-written summary
SlREO expression was high in roots and very low in aerial organs.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- rootse0
- Gene
- SlREOe0
- Outcome
- highly expressed in roots but had a very low level of expression in aerial plant organse0
- Direction
- highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlREO, a novel gene isolated from tomato (Solanum lycopersicum L.). This gene was highly expressed in roots but had a very low level of expression in aerial plant organs.”
Claim · model-written summary
The SlREO promoter directed reporter expression in root cortex.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- root cortexe0
- Gene
- SlREOe0
- Outcome
- direct expression in the root cortexe0
- Value
- 2.4-kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 2.4-kb region representing the SlREO promoter sequence was cloned upstream of the uidA GUS reporter gene and shown to direct expression in the root cortex.”
e1“tomato (Solanum lycopersicum L.)”
Claim · model-written summary
SlREO promoter root specificity persisted in mature glasshouse-grown plants.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- maturee0
- Gene
- SlREOe2
- Outcome
- strict root specificity was maintainede0
- Setting
- glasshouse-growne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mature, glasshouse-grown plants this strict root specificity was maintained.”
e1“tomato (Solanum lycopersicum L.)”
e2“SlREO promoter sequence”
Claim · model-written summary
Dehydration did not affect promoter activity.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- dehydratione0
- Gene
- SlREOe2
- Outcome
- promoter activitye0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoter activity was unaffected by dehydration or wounding stress”
e1“tomato (Solanum lycopersicum L.)”
e2“SlREO promoter sequence”
Claim · model-written summary
Wounding stress did not affect promoter activity.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- wounding stresse0
- Gene
- SlREOe2
- Outcome
- promoter activitye0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoter activity was unaffected by dehydration or wounding stress”
e1“tomato (Solanum lycopersicum L.)”
e2“SlREO promoter sequence”
Claim · model-written summary
NaCl exposure somewhat suppressed promoter activity.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- NaCle0
- Gene
- SlREOe2
- Outcome
- promoter activitye0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoter activity was unaffected by dehydration or wounding stress but was somewhat suppressed by exposure to NaCl, salicylic acid and jasmonic acid.”
e1“tomato (Solanum lycopersicum L.)”
e2“SlREO promoter sequence”
Claim · model-written summary
Salicylic acid exposure somewhat suppressed promoter activity.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- salicylic acide0
- Gene
- SlREOe2
- Outcome
- promoter activitye0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoter activity was unaffected by dehydration or wounding stress but was somewhat suppressed by exposure to NaCl, salicylic acid and jasmonic acid.”
e1“tomato (Solanum lycopersicum L.)”
e2“SlREO promoter sequence”
Claim · model-written summary
Jasmonic acid exposure somewhat suppressed promoter activity.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- jasmonic acide0
- Gene
- SlREOe2
- Outcome
- promoter activitye0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoter activity was unaffected by dehydration or wounding stress but was somewhat suppressed by exposure to NaCl, salicylic acid and jasmonic acid.”
e1“tomato (Solanum lycopersicum L.)”
e2“SlREO promoter sequence”
Claim · model-written summary
Predicted SlREO protein contains a dioxygenase superfamily domain.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlREOe0
- Outcome
- contains a domain found in enzymes of the 2-oxoglutarate and Fe(II)-dependent dioxygenase superfamilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted protein sequence of SlREO contains a domain found in enzymes of the 2-oxoglutarate and Fe(II)-dependent dioxygenase superfamily.”
e1“tomato (Solanum lycopersicum L.)”
Claim · model-written summary
The authors identify the SlREO promoter as useful for strong, root-specific expression in tomato and possibly other Solanaceous crops.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlREOe0
- Outcome
- strong and specific gene expression in the bulk of tomato root tissuee0
- Qualifier
- also likely to be useful in other Solanaceous cropse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The novel SlREO promoter has properties ideal for applications requiring strong and specific gene expression in the bulk of tomato root tissue growing in soil, and is also likely to be useful in other Solanaceous crops.”
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