Abstract
Fusarium wilt caused by Fusarium oxysporum is considered a fatal disease to bananas.Chemical control of Fusarium wilt in bananas has received little efficiency. Alternatively, biological control is a viable strategy against soil-borne diseases. The objectives of this research were to isolate antagonistic bacteria and investigate the possible mechanisms against Fusarium wilt. An antagonistic Bacillus strain, termed KY-21 against F. oxysporumf. sp. Cubense was isolated from the soil of banana’s rhizosphere by dual culture. The strain was identified as Bacillus subtilis according to the characteristics of its morphology and by homology analysis of its 16S rDNA sequence. Mycelium growth of the pathogen was seriously inhibited after treatment with the fermentation filtrate of KY-21. Observation revealed that the tips of the hypha were deformed into spherical structures that were remarkably constricted. The strain KY-21 displayed a good ability to colonize and can transfer into banana tissues. The activities of polyphenol oxidase (PPO) and peroxidase (POD) in bananas showed an increase after inoculated with KY-21, compared to control.In vivo biocontrol assays showed a significant reduction in wilt index and vascular discoloration. These results indicated that the antagonistic mechanisms against Fusariumwilt were involved in the inhibition of mycelial growth and the improvement in activity of defense related enzymes. Key words: Fusarium oxysporum f. sp. Cubense, antagonistic bacteria, screen,colonization, enzyme activity.
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Claims 7
Claim · model-written summary
The fermentation filtrate of KY-21 seriously inhibited mycelial growth of Fusarium oxysporum.
- Taxon
- bananat0
- Treatment
- fermentation filtrate of KY-21e2
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume1pathogen - Second interactor
- KY-21e3biocontrol_agent
- Outcome
- Mycelium growth of the pathogene2
- Direction
- seriously inhibitede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
t0“defense related enzymes in banana”
e1“Fusarium wilt caused by Fusarium oxysporum is considered a fatal disease to bananas”
e2“Mycelium growth of the pathogen was seriously inhibited after treatment with the fermentation filtrate of KY-21”
e3“An antagonistic Bacillus strain, termed KY-21”
Claim · model-written summary
Strain KY-21 showed a good ability to colonize banana tissues and to transfer into them.
Verbatim
t0“defense related enzymes in banana”
e5“The strain KY-21 displayed a good ability to colonize and can transfer into banana tissues”
Claim · model-written summary
Inoculation with KY-21 increased polyphenol oxidase and peroxidase activities in banana compared with control.
Verbatim
t0“defense related enzymes in banana”
e4“The activities of polyphenol oxidase (PPO) and peroxidase (POD) in bananas showed an increase after inoculated with KY-21, compared to control”
Claim · model-written summary
In vivo biocontrol assays gave a significant reduction in wilt index and vascular discoloration of Fusarium-infected banana.
- Taxon
- bananat0
- Treatment
- biocontrol assayse6
- Interactor
- Fusarium oxysporumFusarium oxysporume1pathogen
- Outcome
- wilt index and vascular discoloratione6
- Direction
- reductione6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“defense related enzymes in banana”
e1“Fusarium wilt caused by Fusarium oxysporum is considered a fatal disease to bananas”
e6“In vivo biocontrol assays showed a significant reduction in wilt index and vascular discoloration”
Claim · model-written summary
The antagonistic mechanisms against Fusarium wilt are interpreted as inhibition of mycelial growth together with improved activity of defense-related enzymes.
Verbatim
t0“defense related enzymes in banana”
e7“the antagonistic mechanisms against Fusariumwilt were involved in the inhibition of mycelial growth and the improvement in activity of defense related enzymes”
Claim · model-written summary
The antagonistic strain KY-21 was identified as Bacillus subtilis by morphology and 16S rDNA homology.
- Taxon
- bananat0
- Interactor
- Bacillus subtilis
Bacillus subtilise2biocontrol_agent - Outcome
- identified as Bacillus subtilise2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“defense related enzymes in banana”
e2“The strain was identified as Bacillus subtilis according to the characteristics of its morphology and by homology analysis of its 16S rDNA sequence”
Claim · model-written summary
Hyphal tips of Fusarium oxysporum were deformed into spherical, remarkably constricted structures.
- Taxon
- bananat0
- Interactor
- Fusarium oxysporumFusarium oxysporume1pathogen
- Outcome
- tips of the hypha were deformed into spherical structurese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“defense related enzymes in banana”
e1“Fusarium wilt caused by Fusarium oxysporum is considered a fatal disease to bananas”
e2“Observation revealed that the tips of the hypha were deformed into spherical structures that were remarkably constricted”
The same questions in other papers
inoculation · physiology: antioxidant enzymes · MusaPositive and negative results
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- negative
- no effect
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See all 12 papers →biocontrol · Fusarium oxysporum · MusaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.