Abstract
We report here that disruption of function of the ω-3 FATTY ACID DESATURASE7 (FAD7) enhances plant defenses against aphids. The suppressor of prosystemin-mediated responses2 (spr2) mutation in tomato (Solanum lycopersicum), which eliminates the function of FAD7, reduces the settling behavior, survival, and fecundity of the potato aphid (Macrosiphum euphorbiae). Likewise, the antisense suppression of LeFAD7 expression in wild-type tomato plants reduces aphid infestations. Aphid resistance in the spr2 mutant is associated with enhanced levels of salicylic acid (SA) and mRNA encoding the pathogenesis-related protein P4. Introduction of the Naphthalene/salicylate hydroxylase transgene, which suppresses SA accumulation, restores wild-type levels of aphid susceptibility to spr2. Resistance in spr2 is also lost when we utilize virus-induced gene silencing to suppress the expression of NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1), a positive regulator of many SA-dependent defenses. These results indicate that FAD7 suppresses defenses against aphids that are mediated through SA and NPR1. Although loss of function of FAD7 also inhibits the synthesis of jasmonate (JA), the effects of this desaturase on aphid resistance are not dependent on JA; other mutants impaired in JA synthesis (acx1) or perception (jai1-1) show wild-type levels of aphid susceptibility, and spr2 retains aphid resistance when treated with methyl jasmonate. Thus, FAD7 may influence JA-dependent defenses against chewing insects and SA-dependent defenses against aphids through independent effects on JA synthesis and SA signaling. The Arabidopsis (Arabidopsis thaliana) mutants Atfad7-2 and Atfad7-1fad8 also show enhanced resistance to the green peach aphid (Myzus persicae) compared with wild-type controls, indicating that FAD7 influences plant-aphid interactions in at least two plant families.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Spr2 mutation in tomato reduces settling behavior, survival and fecundity of the potato aphid
- Taxon
- tomato
Solanum lycopersicume1 - Material
- spr2e1
- Treatment
- (spr2) mutatione1
- Interactor
- Macrosiphum euphorbiae
Macrosiphum euphorbiaee1pest - Outcome
- settling behavior, survival, and fecunditye1
- Direction
- reducese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The suppressor of prosystemin-mediated responses2 (spr2) mutation in tomato (Solanum lycopersicum), which eliminates the function of FAD7, reduces the settling behavior, survival, and fecundity of the potato aphid (Macrosiphum euphorbiae)”
Claim · model-written summary
Antisense suppression of LeFAD7 expression in wild-type tomato reduces aphid infestations
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- antisense suppression of LeFAD7 expressione2
- Interactor
- aphide2pest
- Gene
- LeFAD7e2
- Outcome
- aphid infestationse2
- Direction
- reducese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Likewise, the antisense suppression of LeFAD7 expression in wild-type tomato plants reduces aphid infestations”
Claim · model-written summary
Aphid resistance in the spr2 mutant is associated with enhanced salicylic acid levels and enhanced PR P4 mRNA
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- spr2e3
- Interactor
- Aphide3pest
- Outcome
- levels of salicylic acid (SA) and mRNA encoding the pathogenesis-related protein P4e3
- Direction
- enhancede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e3“Aphid resistance in the spr2 mutant is associated with enhanced levels of salicylic acid (SA) and mRNA encoding the pathogenesis-related protein P4”
Claim · model-written summary
Introducing the NahG transgene that suppresses SA accumulation restores wild-type levels of aphid susceptibility in spr2
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- spr2e4
- Treatment
- Introduction of the Naphthalene/salicylate hydroxylase transgenee4
- Interactor
- aphide4pest
- Outcome
- aphid susceptibilitye4
- Direction
- restorese4
- Comparator
- spr2e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e4“Introduction of the Naphthalene/salicylate hydroxylase transgene, which suppresses SA accumulation, restores wild-type levels of aphid susceptibility to spr2”
Claim · model-written summary
Resistance in spr2 is lost when NPR1 is suppressed by virus-induced gene silencing
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- spr2e5
- Treatment
- virus-induced gene silencinge5
- Gene
- NPR1e5
- Outcome
- Resistancee5
- Direction
- loste5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e5“Resistance in spr2 is also lost when we utilize virus-induced gene silencing to suppress the expression of NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1)”
Claim · model-written summary
FAD7 suppresses defenses against aphids that are mediated through SA and NPR1
- Taxon
- TomatoSolanum lycopersicumt0
- Interactor
- aphidsAphidomorphae6pest
- Gene
- FAD7e6
- Outcome
- defenses against aphidse6
- Qualifier
- mediated through SA and NPR1e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e6“These results indicate that FAD7 suppresses defenses against aphids that are mediated through SA and NPR1”
Claim · model-written summary
Mutants impaired in JA synthesis or perception show wild-type levels of aphid susceptibility
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- impaired in JA synthesis (acx1) or perception (jai1-1)e7
- Interactor
- aphide7pest
- Outcome
- aphid susceptibilitye7
- Direction
- no_changeinferrede7
- Comparator
- wild-typee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e7“other mutants impaired in JA synthesis (acx1) or perception (jai1-1) show wild-type levels of aphid susceptibility”
Claim · model-written summary
Spr2 retains aphid resistance when treated with methyl jasmonate
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- spr2e1
- Treatment
- methyl jasmonatee1
- Interactor
- aphide1pest
- Outcome
- aphid resistancee1
- Direction
- retainse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e1“spr2 retains aphid resistance when treated with methyl jasmonate”
Claim · model-written summary
Arabidopsis mutants Atfad7-2 and Atfad7-1fad8 show enhanced resistance to the green peach aphid compared with wild-type controls
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Material
- Atfad7-2 and Atfad7-1fad8e1
- Treatment
- mutants Atfad7-2 and Atfad7-1fad8e1
- Interactor
- Myzus persicae
Myzus persicaee1pest - Outcome
- resistance to the green peach aphide1
- Direction
- enhancede1
- Comparator
- wild-type controlse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The Arabidopsis (Arabidopsis thaliana) mutants Atfad7-2 and Atfad7-1fad8 also show enhanced resistance to the green peach aphid (Myzus persicae) compared with wild-type controls”
Claim · model-written summary
FAD7 influences plant-aphid interactions in at least two plant families
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Interactor
- aphide2pest
- Gene
- FAD7e2
- Outcome
- plant-aphid interactionse2
- Qualifier
- in at least two plant familiese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The Arabidopsis (Arabidopsis thaliana) mutants Atfad7-2 and Atfad7-1fad8 also show enhanced resistance to the green peach aphid (Myzus persicae) compared with wild-type controls”
e2“indicating that FAD7 influences plant-aphid interactions in at least two plant families”
The same questions in other papers
(-)-methyl jasmonate · disease · Solanum lycopersicum (tomato)Positive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
4 papers more in this group
See all 8 papers →loss of function · Macrosiphum euphorbiae · Solanum lycopersicum (tomato)Positive and negative results
1 in the opposite direction
- no effect
loss of function · disease · Solanum lycopersicum (tomato)Positive and negative results
91 papers in the same direction as this paper · 63 in the opposite direction
- positive
- positive
- negative
151 papers more in this group
See all 155 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.