Abstract
ABSTRACT Two genes with a common region that is characteristic of the TPSI1/Mt4 family were cloned from a Pi‐starvation‐induced cDNA library of rice roots using suppression subtracted hybridization (SSH). Based on the consensus sequence of these two genes, members of the TPSI1/Mt4 family were found in maize, wheat and barley. blast and a cluster analysis in the eight members of the TPSI1/Mt4 family showed two classes of four genes each among monocots. The first gene from rice was designated OsIPS1 based on a comparison of the consensus sequence with AtIPS1 , and consequently the second gene, which has been previously reported as OsPI1 , was designated OsIPS2 . Accumulation of the mRNA of OsIPS1/2 was examined by northern blotting and quantitative reverse transcriptase‐polymerase chain reaction in whole‐root and split‐root experiments under treatment with phosphate (Pi) and the Pi analogue phosphite (Phi). OsIPS1 showed much higher mRNA accumulation in roots than OsIPS2 , and an opposite trend was seen in shoots. OsIPS1/2 showed both systemic and local responses to Pi starvation, and less than 10% of the overall induced mRNA level was due to the local Pi concentration in roots. The results indicate that Phi may interfere with earlier events in roots that are associated with a local Pi signalling pathway. An analysis of transgenic plants showed that OsIPS1/2 are independently responsive to Pi signalling and are mainly expressed in lateral roots and in the vascular cylinder in the primary root. Exogenous cytokinin (6‐BA) almost completely suppressed systemic Pi starvation signalling and partially suppressed local Pi signalling. Exogenous abscisic acid remarkably reduced Pi starvation signalling. In contrast, exogenous auxin enhanced Pi signalling, especially local Pi signalling in roots. Exogenous ethylene (ethyphon) and the ratio of auxin to cytokinins did not appear to affect the expression of these two genes.
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Claims 10
Claim · model-written summary
OsIPS1 showed much higher mRNA accumulation in roots than OsIPS2, with an opposite trend in shoots.
- Taxon
- rice
Oryza sativat0 - Organ / stage
- rootse1
- Gene
- OsIPS1e1
- Outcome
- mRNA accumulatione1
- Direction
- much highere1
- Comparator
- OsIPS2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e1“OsIPS1 showed much higher mRNA accumulation in roots than OsIPS2, and an opposite trend was seen in shoots”
Claim · model-written summary
OsIPS1/2 showed both systemic and local responses to Pi starvation.
- Taxon
- riceOryza sativat0
- Treatment
- Pi starvatione2
- Gene
- OsIPS1/2e2
- Outcome
- systemic and local responsese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e2“OsIPS1/2 showed both systemic and local responses to Pi starvation, and less than 10% of the overall induced mRNA level was due to the local Pi concentration in roots”
Claim · model-written summary
Less than 10% of the overall induced mRNA level of OsIPS1/2 was due to the local Pi concentration in roots.
- Taxon
- riceOryza sativat0
- Treatment
- local Pi concentratione2
- Gene
- OsIPS1/2e2
- Outcome
- overall induced mRNA levele2
- Value
- less than 10%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e2“OsIPS1/2 showed both systemic and local responses to Pi starvation, and less than 10% of the overall induced mRNA level was due to the local Pi concentration in roots”
Claim · model-written summary
Phosphite may interfere with earlier events in roots that are associated with a local Pi signalling pathway.
- Taxon
- riceOryza sativat0
- Treatment
- Phie3
- Outcome
- earlier events in rootse3
- Qualifier
- local Pi signalling pathwaye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e3“The results indicate that Phi may interfere with earlier events in roots that are associated with a local Pi signalling pathway”
Claim · model-written summary
OsIPS1/2 are mainly expressed in lateral roots and in the vascular cylinder of the primary root.
- Taxon
- riceOryza sativat0
- Organ / stage
- lateral rootse4
- Gene
- OsIPS1/2e4
- Outcome
- expressede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e4“An analysis of transgenic plants showed that OsIPS1/2 are independently responsive to Pi signalling and are mainly expressed in lateral roots and in the vascular cylinder in the primary root”
Claim · model-written summary
Exogenous cytokinin almost completely suppressed systemic Pi starvation signalling.
- Taxon
- riceOryza sativat0
- Treatment
- Exogenous cytokinine5
- Compound
- cytokinine5
- Outcome
- systemic Pi starvation signallinge5
- Direction
- almost completely suppressede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e5“Exogenous cytokinin (6‐BA) almost completely suppressed systemic Pi starvation signalling and partially suppressed local Pi signalling”
Claim · model-written summary
Exogenous cytokinin partially suppressed local Pi signalling.
- Taxon
- riceOryza sativat0
- Treatment
- Exogenous cytokinine5
- Compound
- cytokinine5
- Outcome
- local Pi signallinge5
- Direction
- partially suppressede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e5“Exogenous cytokinin (6‐BA) almost completely suppressed systemic Pi starvation signalling and partially suppressed local Pi signalling”
Claim · model-written summary
Exogenous abscisic acid remarkably reduced Pi starvation signalling.
- Taxon
- riceOryza sativat0
- Treatment
- Exogenous abscisic acide6
- Compound
- abscisic acide6
- Outcome
- Pi starvation signallinge6
- Direction
- remarkably reducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e6“Exogenous abscisic acid remarkably reduced Pi starvation signalling”
Claim · model-written summary
Exogenous auxin enhanced local Pi signalling in roots.
- Taxon
- riceOryza sativat0
- Treatment
- exogenous auxine7
- Compound
- auxine7
- Outcome
- local Pi signalling in rootse7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e7“In contrast, exogenous auxin enhanced Pi signalling, especially local Pi signalling in roots”
Claim · model-written summary
Exogenous ethylene and the auxin-to-cytokinin ratio did not appear to affect OsIPS1/2 expression.
- Taxon
- riceOryza sativat0
- Treatment
- Exogenous ethylene (ethyphon)e6
- Outcome
- the expressione6
- Direction
- did not appear to affecte6
- Qualifier
- the ratio of auxin to cytokininse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“OsIPS1 and OsIPS2 in rice”
e6“Exogenous ethylene (ethyphon) and the ratio of auxin to cytokinins did not appear to affect the expression of these two genes”
The same questions in other papers
ethene · molecular: expression · Oryza sativa (rice)Positive and negative results
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