Paper · 1998 · Wuhan University Journal of Natural Sciences

Changes of levels of glutamine synthetase isoforms in roots and leaves in response to nitrogen fertilizer application at different growth stages in irrigated rice

Zhang Chufu; Peng Shaobing; John Bennett

10
claims
14
verbatim quotes
1
citations
3
research areas
—
Not open access

Abstract

Nitrogen is a key element to control the growth and yield of crops. Fertilizer urea nitrogen (N) 60,45, and 30 kg/hm2 was applied at three different stages, midtillering, panicle initiation, and flowering, of the growth and development of rice plants, respectively. At both midtillering and panicle initiation, the total activity of glutamine synthetase (GS) in rice roots and leaves was incrased remarkably as a result of a large amount of ammonia absorbed by roots. Native-PAGE and activity staining showed that the increase of total activity in rice roots and leaves was due to the synthesis of GSrb in roots and GS2 in leaves and that the activity of GSra in roots and GS1 in leaves remained constant. The results showed that the assimilation of external nitrogen was carried out by GSrb but not GSra in rice roots and that the activitry of GS2 was induced also by the external nitrogen, and that GSrb played main role in meeting the needs of the rapid tillering for nitrogen. At flowering, the activity of GS in rice roots and leaves did not change almost after topdressing. These rssults suggest that the change of GS activity in rice roots may use as a measure of the utilization efficiency of the fertilizer.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At midtillering and panicle initiation, nitrogen fertilizer markedly increased total GS activity in rice roots and leaves.

Organ / stage
midtillering and panicle initiatione0
Treatment
ammonia absorbed by rootse0
Outcome
total activity of glutamine synthetase (GS) in rice roots and leavese0
Direction
incrasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“At both midtillering and panicle initiation, the total activity of glutamine synthetase (GS) in rice roots and leaves was incrased remarkably as a result of a large amount of ammonia absorbed by roots.”

✓ located verbatim in the source · abstract, characters 266–467 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Increased root GS activity was attributed to synthesis of GSrb.

Organ / stage
rootse0
Gene
GSrbe0
Outcome
increase of total activity in rice rootse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the increase of total activity in rice roots and leaves was due to the synthesis of GSrb in roots and GS2 in leaves”

✓ located verbatim in the source · abstract, characters 514–629 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Increased leaf GS activity was attributed to synthesis of GS2.

Organ / stage
leavese0
Gene
GS2e0
Outcome
increase of total activity in rice roots and leavese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the increase of total activity in rice roots and leaves was due to the synthesis of GSrb in roots and GS2 in leaves”

✓ located verbatim in the source · abstract, characters 514–629 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Root GSra activity remained constant.

Gene
GSrae0
Outcome
activity of GSra in rootse0
Direction
remained constante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the activity of GSra in roots and GS1 in leaves remained constant.”

✓ located verbatim in the source · abstract, characters 639–705 · show in text ↑
t0

“rice”

✓ located verbatim in the source · title, characters 158–162 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Leaf GS1 activity remained constant.

Gene
GS1e0
Outcome
GS1 in leavese0
Direction
remained constante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the activity of GSra in roots and GS1 in leaves remained constant.”

✓ located verbatim in the source · abstract, characters 639–705 · show in text ↑
t0

“rice”

✓ located verbatim in the source · title, characters 158–162 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

GSrb, rather than GSra, assimilated external nitrogen in rice roots.

Organ / stage
rootse0
Gene
GSrbe0
Outcome
assimilation of external nitrogene0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the assimilation of external nitrogen was carried out by GSrb but not GSra in rice roots”

✓ located verbatim in the source · abstract, characters 730–818 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

External nitrogen induced GS2 activity.

Treatment
external nitrogene0
Gene
GS2e0
Outcome
activitry of GS2e0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the activitry of GS2 was induced also by the external nitrogen”

✓ located verbatim in the source · abstract, characters 828–890 · show in text ↑
t0

“rice”

✓ located verbatim in the source · title, characters 158–162 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

GSrb played a main role in supplying nitrogen for rapid tillering.

Gene
GSrbe0
Outcome
meeting the needs of the rapid tillering for nitrogene0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“GSrb played main role in meeting the needs of the rapid tillering for nitrogen.”

✓ located verbatim in the source · abstract, characters 901–980 · show in text ↑
t0

“rice”

✓ located verbatim in the source · title, characters 158–162 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

At flowering, topdressing left GS activity in rice roots and leaves almost unchanged.

Organ / stage
floweringe0
Treatment
topdressinge0
Outcome
activity of GS in rice roots and leavese0
Direction
did not change almoste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“At flowering, the activity of GS in rice roots and leaves did not change almost after topdressing.”

✓ located verbatim in the source · abstract, characters 981–1079 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors suggest root GS activity changes as a measure of fertilizer utilization efficiency.

Outcome
change of GS activity in rice roots may use as a measure of the utilization efficiency of the fertilizere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“the change of GS activity in rice roots may use as a measure of the utilization efficiency of the fertilizer.”

✓ located verbatim in the source · abstract, characters 1107–1216 · show in text ↑

ammonia · molecular: metabolites · Oryza sativa (rice)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. negative
  2. negative
See all 3 papers →

fertilization · molecular: metabolites · Oryza sativa (rice)Positive and negative results

219 papers: 199 positive, 20 negative or no effect

19 papers in the same direction as this paper · 199 in the opposite direction

  1. positive
  2. positive
  3. positive

215 papers more in this group

See all 219 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.