Abstract
1. The effects of manipulative procedures on intrastrain variation in excised roots of the Dorset Marlgrass strain of red clover (Trifolium pratense L.) have been studied, and a technique established for the satisfactory maintenance of two derived excised root clones. 2. A study has been made, using roots of clone 1, of the influence of sucrose concentration of the medium on growth of roots cultured 8 days and upon survival of growth activity in individual meristems when repeatedly subcultured. Main-axis meristems have a lower optimum sucrose concentration than do lateral meristems. At the standard 2% sucrose concentration nearly all lateral roots are robust and opaque. They remain opaque and increase in diameter with increase in the sucrose concentration. At 0.75% or lower sucrose concentrations, all the lateral roots are fine and transparent. 3. Roots which develop from tips excised from these robust and fine laterals differ in growth rate, morphology, and survival of their main-axis meristems. Attempts to obtain the normal robust-type roots from the tips of fine laterals have been unsuccessful. 4. Persistence of growth activity in main-axis meristems is enhanced by lowering the sucrose concentration of the medium and, in robust roots, by reducing the interval between successive subcultures. Continuous growth of individual meristems cannot, however, be achieved by a combination of these two effects. 5. Clone 2 roots clearly differ in growth and morphology from those of clone 1. Clone 2 roots give rise more readily and at higher sucrose concentrations to fine-type laterals. In growth rate, response to sucrose concentration, and survival of growth activity when individual meristems are repeatedly subcultured, the fine- and robust-type roots of clone 2 differ markedly from one another and from the corresponding types of clone 1 roots. 6. A hypothesis of the hormonal control of meristematic activity in root-tip meristems is discussed relative to these findings and to those from previous studies on clones of excised tomato roots.
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Claims 13
Claim · model-written summary
A technique maintained two derived excised root clones satisfactorily.
- Taxon
- Red Clover
Trifolium pratenset0 - Outcome
- satisfactory maintenance of two derived excised root clonese0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a technique established for the satisfactory maintenance of two derived excised root clones”
t0“Red Clover”
Claim · model-written summary
Main-axis meristems had a lower optimal sucrose concentration than lateral meristems.
- Taxon
- Red CloverTrifolium pratenset0
- Organ / stage
- Main-axis meristemse0
- Treatment
- sucrose concentratione0
- Outcome
- optimum sucrose concentratione0
- Direction
- lowere0
- Comparator
- lateral meristemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Main-axis meristems have a lower optimum sucrose concentration than do lateral meristems”
t0“Red Clover”
Claim · model-written summary
At 2% sucrose nearly all lateral roots were robust and opaque.
- Taxon
- Red CloverTrifolium pratenset0
- Organ / stage
- lateral rootse0
- Treatment
- 2% sucrose concentratione0
- Outcome
- robust and opaquee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the standard 2% sucrose concentration nearly all lateral roots are robust and opaque”
t0“Red Clover”
Claim · model-written summary
Lateral roots increased in diameter as sucrose concentration increased.
- Taxon
- Red CloverTrifolium pratenset0
- Organ / stage
- lateral rootse1
- Treatment
- sucrose concentratione0
- Outcome
- diametere0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They remain opaque and increase in diameter with increase in the sucrose concentration”
t0“Red Clover”
e1“At the standard 2% sucrose concentration nearly all lateral roots are robust and opaque”
Claim · model-written summary
At sucrose concentrations of 0.75% or lower, lateral roots were fine and transparent.
- Taxon
- Red CloverTrifolium pratenset0
- Organ / stage
- lateral rootse0
- Treatment
- 0.75% or lower sucrose concentrationse0
- Outcome
- fine and transparente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 0.75% or lower sucrose concentrations, all the lateral roots are fine and transparent”
t0“Red Clover”
Claim · model-written summary
Roots arising from robust and fine lateral tips differed in growth rate, morphology and main-axis meristem survival.
- Taxon
- Red CloverTrifolium pratenset0
- Material
- robust and fine lateralse0
- Outcome
- growth rate, morphology, and survival of their main-axis meristemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Roots which develop from tips excised from these robust and fine laterals differ in growth rate, morphology, and survival of their main-axis meristems”
t0“Red Clover”
Claim · model-written summary
Attempts to derive robust-type roots from fine lateral tips were unsuccessful.
- Taxon
- Red CloverTrifolium pratenset0
- Organ / stage
- tips of fine lateralse0
- Outcome
- obtain the normal robust-type rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Attempts to obtain the normal robust-type roots from the tips of fine laterals have been unsuccessful”
t0“Red Clover”
Claim · model-written summary
Lowering medium sucrose enhanced persistence of main-axis meristem growth activity.
- Taxon
- Red CloverTrifolium pratenset0
- Organ / stage
- main-axis meristemse0
- Treatment
- lowering the sucrose concentration of the mediume0
- Outcome
- Persistence of growth activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Persistence of growth activity in main-axis meristems is enhanced by lowering the sucrose concentration of the medium”
t0“Red Clover”
Claim · model-written summary
Shorter intervals between subcultures enhanced main-axis meristem activity in robust roots.
- Taxon
- Red CloverTrifolium pratenset0
- Material
- robust rootse0
- Treatment
- reducing the interval between successive subculturese0
- Outcome
- growth activitye1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in robust roots, by reducing the interval between successive subcultures”
t0“Red Clover”
e1“Persistence of growth activity in main-axis meristems is enhanced by lowering the sucrose concentration of the medium”
Claim · model-written summary
Combining lower sucrose and shorter subculture intervals did not achieve continuous meristem growth.
- Taxon
- Red CloverTrifolium pratenset0
- Treatment
- combination of these two effectse0
- Outcome
- Continuous growth of individual meristemse0
- Direction
- cannote0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Continuous growth of individual meristems cannot, however, be achieved by a combination of these two effects”
t0“Red Clover”
Claim · model-written summary
Clone 2 roots differed from clone 1 in growth and morphology.
- Taxon
- Red CloverTrifolium pratenset0
- Material
- Clone 2e0
- Outcome
- growth and morphologye0
- Comparator
- clone 1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clone 2 roots clearly differ in growth and morphology from those of clone 1”
t0“Red Clover”
Claim · model-written summary
Clone 2 produced fine-type laterals more readily and at higher sucrose concentrations than clone 1.
- Taxon
- Red CloverTrifolium pratenset0
- Material
- Clone 2e0
- Organ / stage
- fine-type lateralse0
- Treatment
- sucrose concentrationse0
- Outcome
- give rise more readily and at higher sucrose concentrations to fine-type lateralse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clone 2 roots give rise more readily and at higher sucrose concentrations to fine-type laterals”
t0“Red Clover”
Claim · model-written summary
Fine and robust roots of clone 2 differed from each other and corresponding clone 1 roots in growth rate, sucrose response and meristem activity survival.
- Taxon
- Red CloverTrifolium pratenset0
- Material
- clone 2e0
- Outcome
- growth rate, response to sucrose concentration, and survival of growth activitye0
- Comparator
- clone 1 rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In growth rate, response to sucrose concentration, and survival of growth activity when individual meristems are repeatedly subcultured, the fine- and robust-type roots of clone 2 differ markedly from one another and from the corresponding types of clone 1 roots”
t0“Red Clover”
The same questions in other papers
sucrose · growth · Trifolium pratense (red clover)Results agree
2 papers in the same direction as this paper
- positive
- positive
in vitro · growth · Trifolium pratense (red clover)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.