Abstract
The effect of glutathione on the influences of heavy metals affecting rubisco and rubisco activase was studied in tobacco plants grown in vitro where the shoot explants of the tobacco plant cultured on MS medium under aseptic conditions and two explants were placed in the control, 0.1 mM GSH, 1 mM GSH, 0.2 mM Cd, 0.2 mM Cu, 0.2 mM Zn, and a mixture of Cd and GSH, Cu and GSH, Zn and GSH, respectively. The effect of GSH on the growth of the tobacco plant was minimal, but the heavy metals clearly retarded its growth. GSH recovered the growth retarded by heavy metals, and the concentration of GSH required to recover the growth differed depending on the heavy metals. The content of chlorophyll in the plant increased through GSH and Zn, and decreased through Cd and Cu. The chlorophyll content which decreased due to Cd and Cu was recovered by GSH, and the content which increased due to Zn was decreased by 1 mM GSH. The content of rubisco decreased due to GSH and heavy metals, and the content which decreased due to heavy metals was recovered by GSH, and when GSH was treated with Zn, the increased rate was maximum compared to other heavy metals. The activity of rubisco was increased due to GSH and heavy metals, and the activity increased by Cd and Zn decreased through GSH. In the case of Cu, the activity of GSH increased even more. There was no effect of GSH on the influences of heavy metals on the content and activity of rubisco activase. The activity of rubisco decreased by thiourea among six denaturing agents, and increased by l-cysteine, and in most cases the activity level was recorded as high. The activity of rubisco activase all decreased as a result of six denaturing agents, and the effect caused by EDTA and guanidine-HCl was the greatest, while the effect caused by l-cysteine and urea was minimal.
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Claims 20
Claim · model-written summary
GSH had minimal effect on tobacco growth.
- Taxon
- tobacco plant
Nicotiana tabacumt0 - Treatment
- GSHe0
- Outcome
- growthe0
- Direction
- minimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The effect of GSH on the growth of the tobacco plant was minimal”
Claim · model-written summary
Heavy metals retarded tobacco growth.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- heavy metalse0
- Outcome
- growthe0
- Direction
- retardede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“the heavy metals clearly retarded its growth”
Claim · model-written summary
GSH restored tobacco growth retarded by heavy metals.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Outcome
- growthe0
- Direction
- recoverede0
- Comparator
- heavy metalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“GSH recovered the growth retarded by heavy metals”
Claim · model-written summary
The GSH concentration needed to restore growth differed by heavy metal.
- Taxon
- tobacco plantNicotiana tabacumt0
- Outcome
- concentration of GSH required to recover the growthe0
- Qualifier
- depending on the heavy metalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“the concentration of GSH required to recover the growth differed depending on the heavy metals”
Claim · model-written summary
GSH increased tobacco chlorophyll content.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Outcome
- content of chlorophylle0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The content of chlorophyll in the plant increased through GSH and Zn”
Claim · model-written summary
Zn increased tobacco chlorophyll content.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- Zne0
- Outcome
- content of chlorophylle0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The content of chlorophyll in the plant increased through GSH and Zn”
Claim · model-written summary
Cd decreased tobacco chlorophyll content.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- Cde0
- Outcome
- content of chlorophylle1
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“decreased through Cd and Cu”
e1“The content of chlorophyll in the plant increased through GSH and Zn”
Claim · model-written summary
Cu decreased tobacco chlorophyll content.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- Cue0
- Outcome
- content of chlorophylle1
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“decreased through Cd and Cu”
e1“The content of chlorophyll in the plant increased through GSH and Zn”
Claim · model-written summary
GSH restored chlorophyll content decreased by Cd and Cu.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Outcome
- chlorophyll contente0
- Direction
- recoverede0
- Comparator
- Cd and Cue0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The chlorophyll content which decreased due to Cd and Cu was recovered by GSH”
Claim · model-written summary
1 mM GSH decreased Zn-elevated chlorophyll content.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- 1 mM GSHe0
- Outcome
- chlorophyll contente1
- Direction
- decreasede0
- Comparator
- Zne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“the content which increased due to Zn was decreased by 1 mM GSH”
e1“The chlorophyll content which decreased due to Cd and Cu was recovered by GSH”
Claim · model-written summary
GSH decreased rubisco content.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Gene
- rubiscoe0
- Outcome
- content of rubiscoe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The content of rubisco decreased due to GSH and heavy metals”
Claim · model-written summary
Heavy metals decreased rubisco content.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- heavy metalse0
- Gene
- rubiscoe0
- Outcome
- content of rubiscoe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The content of rubisco decreased due to GSH and heavy metals”
Claim · model-written summary
GSH restored rubisco content reduced by heavy metals.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Gene
- rubiscoe1
- Outcome
- content of rubiscoe1
- Direction
- recoverede0
- Comparator
- heavy metalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“the content which decreased due to heavy metals was recovered by GSH”
e1“The content of rubisco decreased due to GSH and heavy metals”
Claim · model-written summary
The rubisco content increase with GSH plus Zn was greatest among the heavy-metal combinations.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSH was treated with Zne0
- Gene
- rubiscoe1
- Outcome
- content of rubiscoe1
- Direction
- maximume0
- Comparator
- other heavy metalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“when GSH was treated with Zn, the increased rate was maximum compared to other heavy metals”
e1“The content of rubisco decreased due to GSH and heavy metals”
Claim · model-written summary
GSH increased rubisco activity.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Gene
- rubiscoe0
- Outcome
- activity of rubiscoe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The activity of rubisco was increased due to GSH and heavy metals”
Claim · model-written summary
Heavy metals increased rubisco activity.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- heavy metalse0
- Gene
- rubiscoe0
- Outcome
- activity of rubiscoe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The activity of rubisco was increased due to GSH and heavy metals”
Claim · model-written summary
GSH decreased rubisco activity raised by Cd and Zn.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Gene
- rubiscoe1
- Outcome
- activity of rubiscoe1
- Direction
- decreasede0
- Comparator
- Cd and Zne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“the activity increased by Cd and Zn decreased through GSH”
e1“The activity of rubisco was increased due to GSH and heavy metals”
Claim · model-written summary
With Cu, GSH further increased rubisco activity.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Gene
- rubiscoe1
- Outcome
- activity of rubiscoe1
- Direction
- increasede0
- Comparator
- Cue0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“In the case of Cu, the activity of GSH increased even more”
e1“The activity of rubisco was increased due to GSH and heavy metals”
Claim · model-written summary
GSH did not alter heavy-metal effects on rubisco activase content and activity.
- Taxon
- tobacco plantNicotiana tabacumt0
- Treatment
- GSHe0
- Gene
- rubisco activasee0
- Outcome
- content and activity of rubisco activasee0
- Direction
- no effecte0
- Comparator
- heavy metalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“There was no effect of GSH on the influences of heavy metals on the content and activity of rubisco activase”
Claim · model-written summary
Thiourea decreased rubisco activity, whereas l-cysteine increased it; the six denaturants decreased rubisco activase activity, most with EDTA and guanidine-HCl and least with l-cysteine and urea.
- Taxon
- tobacco plantNicotiana tabacumt0
- Outcome
- activity of rubiscoe0
- Qualifier
- six denaturing agentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco plant”
e0“The activity of rubisco decreased by thiourea among six denaturing agents, and increased by l-cysteine, and in most cases the activity level was recorded as high. The activity of rubisco activase all decreased as a result of six denaturing agents, and the effect caused by EDTA and guanidine-HCl was the greatest, while the effect caused by l-cysteine and urea was minimal.”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.