Abstract
6-Benzylaminopurine, 6-(Delta(2)-isopentenylamino)purine, 6-furfurylaminopurine, rotenone, and antimycin A inhibited oxidation of NADH by mitochondrial sonicates or submitochondrial particles (but not by intact mitochondria) from pea (Pisum sativum L., cult. Alaska) stems and mung bean (Vigna radiata L. Wilczak) hypocotyls. The above purine cytokinins can interfere with electron transport from NADH to the cytochrome system in the inner mitochondrial membrane. Adenine did not inhibit oxidation by sonicated mitochondria, and zeatin was almost ineffective. Zeatin scarcely inhibited state 3 malate respiration by intact mitochondria, but the O-formyl and O-n-propionyl esters of zeatin and the O-acetyl ester of 2-chlorozeatin were more active. Perhaps zeatin is ineffective because it does not get into the inner membranes of the isolated mitochondria, whereas the esters and other cytokinins mentioned above do. N-4-(2-chloropyridyl)-N'-Phenylurea, which has cytokinin-like effects on plant growth and development, inhibited NADH oxidation by sonicated mitochondria. It also inhibited malate, succinate, and NADH oxidation by intact mitochondria; in contrast, the latter two oxidations were not decreased by purine cytokinins.The benzyl, isopentenyl, and furfuryl aminopurines inhibited malate (but not succinate) oxidation by rat liver mitochondria, if ADP was present. In the absence of ADP, these cytokinins promoted succinate (but not malate) oxidation. Zeatin and adenine had slight, if any, effect in either situation.
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Claims 11
Claim · model-written summary
Three purine cytokinins inhibited NADH oxidation in disrupted pea and mung bean mitochondria.
- Taxon
- pea (Pisum sativum L., cult. Alaska)
Lathyrus oleraceuse0 - Organ / stage
- stemse0
- Treatment
- 6-Benzylaminopurinee0
- Outcome
- oxidation of NADHe0
- Direction
- inhibitede0
- Comparator
- intact mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6-Benzylaminopurine, 6-(Delta(2)-isopentenylamino)purine, 6-furfurylaminopurine, rotenone, and antimycin A inhibited oxidation of NADH by mitochondrial sonicates or submitochondrial particles (but not by intact mitochondria) from pea (Pisum sativum L., cult. Alaska) stems and mung bean (Vigna radiata L. Wilczak) hypocotyls.”
Claim · model-written summary
Purine cytokinins inhibited NADH oxidation in mung bean hypocotyl mitochondrial preparations.
- Taxon
- mung bean (Vigna radiata L. Wilczak)
.jpg?width=60)
Vigna radiatae0 - Organ / stage
- hypocotylse0
- Treatment
- 6-(Delta(2)-isopentenylamino)purinee0
- Outcome
- oxidation of NADHe0
- Direction
- inhibitede0
- Comparator
- intact mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“6-Benzylaminopurine, 6-(Delta(2)-isopentenylamino)purine, 6-furfurylaminopurine, rotenone, and antimycin A inhibited oxidation of NADH by mitochondrial sonicates or submitochondrial particles (but not by intact mitochondria) from pea (Pisum sativum L., cult. Alaska) stems and mung bean (Vigna radiata L. Wilczak) hypocotyls.”
Claim · model-written summary
Purine cytokinins can interfere with electron transport from NADH to the cytochrome system.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- purine cytokininse0
- Outcome
- electron transport from NADH to the cytochrome systeme0
- Qualifier
- inner mitochondrial membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The above purine cytokinins can interfere with electron transport from NADH to the cytochrome system in the inner mitochondrial membrane.”
e1“pea (Pisum sativum L., cult. Alaska)”
Claim · model-written summary
Adenine did not inhibit oxidation in sonicated plant mitochondria.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- Adeninee0
- Outcome
- oxidatione0
- Direction
- did not inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adenine did not inhibit oxidation by sonicated mitochondria”
e1“pea (Pisum sativum L., cult. Alaska)”
Claim · model-written summary
Zeatin was almost ineffective for oxidation by sonicated plant mitochondria.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- zeatine0
- Outcome
- ineffectivee0
- Direction
- almost ineffectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“zeatin was almost ineffective”
e1“pea (Pisum sativum L., cult. Alaska)”
Claim · model-written summary
Zeatin scarcely inhibited state 3 malate respiration in intact plant mitochondria.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- Zeatine0
- Outcome
- state 3 malate respiratione0
- Direction
- scarcely inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zeatin scarcely inhibited state 3 malate respiration by intact mitochondria”
e1“pea (Pisum sativum L., cult. Alaska)”
Claim · model-written summary
Zeatin esters were more active than zeatin for state 3 malate respiration.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- O-formyl and O-n-propionyl esters of zeatine0
- Outcome
- more activee0
- Direction
- more activee0
- Comparator
- zeatine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the O-formyl and O-n-propionyl esters of zeatin and the O-acetyl ester of 2-chlorozeatin were more active”
e1“pea (Pisum sativum L., cult. Alaska)”
Claim · model-written summary
The authors suggest zeatin is ineffective because it cannot enter inner mitochondrial membranes.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- zeatine0
- Outcome
- zeatin is ineffectivee0
- Qualifier
- does not get into the inner membranes of the isolated mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Perhaps zeatin is ineffective because it does not get into the inner membranes of the isolated mitochondria”
e1“pea (Pisum sativum L., cult. Alaska)”
Claim · model-written summary
N-4-(2-chloropyridyl)-N'-Phenylurea inhibited NADH oxidation in sonicated plant mitochondria.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- N-4-(2-chloropyridyl)-N'-Phenylureae0
- Outcome
- NADH oxidatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N-4-(2-chloropyridyl)-N'-Phenylurea, which has cytokinin-like effects on plant growth and development, inhibited NADH oxidation by sonicated mitochondria.”
e1“pea (Pisum sativum L., cult. Alaska)”
Claim · model-written summary
N-4-(2-chloropyridyl)-N'-Phenylurea inhibited malate, succinate and NADH oxidation in intact mitochondria.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- N-4-(2-chloropyridyl)-N'-Phenylureae0
- Outcome
- malate, succinate, and NADH oxidatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N-4-(2-chloropyridyl)-N'-Phenylurea, which has cytokinin-like effects on plant growth and development, inhibited NADH oxidation by sonicated mitochondria. It also inhibited malate, succinate, and NADH oxidation by intact mitochondria”
e1“pea (Pisum sativum L., cult. Alaska)”
Claim · model-written summary
Purine cytokinins did not decrease succinate or NADH oxidation in intact mitochondria.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- purine cytokininse0
- Outcome
- the latter two oxidationse0
- Direction
- not decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the latter two oxidations were not decreased by purine cytokinins”
e1“pea (Pisum sativum L., cult. Alaska)”
The same questions in other papers
N-benzyladenine · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.