Abstract
The use of plants for rehabilitation of heavy-metal-contaminated environments is an emerging area of interest because it provides an ecologically sound and safe method for restoration and remediation. Although a number of plant species are capable of hyperaccumulation of heavy metals, the technology is not applicable for remediating sites with multiple contaminants. A clever solution is to combine the advantages of microbe-plant symbiosis within the plant rhizosphere into an effective cleanup technology. We demonstrated that expression of a metal-binding peptide (EC20) in a rhizobacterium, Pseudomonas putida 06909, not only improved cadmium binding but also alleviated the cellular toxicity of cadmium. More importantly, inoculation of sunflower roots with the engineered rhizobacterium resulted in a marked decrease in cadmium phytotoxicity and a 40% increase in cadmium accumulation in the plant root. Owing to the significantly improved growth characteristics of both the rhizobacterium and plant, the use of EC20-expressing P. putida endowed with organic-degrading capabilities may be a promising strategy to remediate mixed organic-metal-contaminated sites.
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Claims 5
Claim · model-written summary
EC20 expression improved cadmium binding by the rhizobacterium.
- Treatment
- expression of a metal-binding peptide (EC20)e0
- Gene
- EC20e0
- Outcome
- cadmium bindinge0
- Direction
- improvede0
Not reportedTaxonComparator
Verbatim
e0“expression of a metal-binding peptide (EC20) in a rhizobacterium, Pseudomonas putida 06909, not only improved cadmium binding”
Claim · model-written summary
EC20 expression alleviated cadmium cellular toxicity in the rhizobacterium.
- Treatment
- expression of a metal-binding peptide (EC20)e0
- Gene
- EC20e0
- Outcome
- cellular toxicity of cadmiume0
- Direction
- alleviatede0
Not reportedTaxonComparator
Verbatim
e0“expression of a metal-binding peptide (EC20) in a rhizobacterium, Pseudomonas putida 06909, not only improved cadmium binding but also alleviated the cellular toxicity of cadmium”
Claim · model-written summary
Inoculation with engineered rhizobacteria markedly decreased cadmium phytotoxicity in sunflower.
- Taxon
- sunflower
Helianthus annuuse0 - Organ / stage
- rootse0
- Treatment
- inoculation of sunflower roots with the engineered rhizobacteriume0
- Interactor
- engineered rhizobacteriume0symbiont
- Outcome
- cadmium phytotoxicitye0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculation of sunflower roots with the engineered rhizobacterium resulted in a marked decrease in cadmium phytotoxicity”
Claim · model-written summary
Inoculation with engineered rhizobacteria increased cadmium accumulation in sunflower roots by 40%.
- Taxon
- sunflowerHelianthus annuuse0
- Treatment
- inoculation of sunflower roots with the engineered rhizobacteriume0
- Interactor
- engineered rhizobacteriume0symbiont
- Outcome
- cadmium accumulation in the plant roote0
- Direction
- increasee0
- Value
- 40%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculation of sunflower roots with the engineered rhizobacterium resulted in a marked decrease in cadmium phytotoxicity and a 40% increase in cadmium accumulation in the plant root”
Claim · model-written summary
The authors propose EC20-expressing P. putida for remediation of mixed organic-metal-contaminated sites.
- Outcome
- use of EC20-expressing P. putida endowed with organic-degrading capabilitiese0
- Qualifier
- mixed organic-metal-contaminated sitese0
Not reportedTaxon
Verbatim
e0“the use of EC20-expressing P. putida endowed with organic-degrading capabilities may be a promising strategy to remediate mixed organic-metal-contaminated sites”
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