Abstract
Seedling emergence is an important trait that can limit commercialization of sweet corn hybrids. This study was designed to test what effect beneficial QTL alleles that enhance seedling emergence exert when introgressed, using marker-assisted backcrossing, into sweet corn commercial germplasm. Three RFLP marker alleles linked to QTL that enhanced seedling emergence were identified in an F(2:3) sweet corn mapping population. A recombinant inbred line (RIL, F(8)) derived from this population was used as a donor parent to backcross the marker-QTL alleles into three elite commercial sweet corn inbreds. Plants in the three segregating BC(2) populations were crossed to the non-recurrent commercial inbreds to produce three BC(2)F(1) populations with families either segregating or lacking the marker donor allele(s). These three populations were evaluated for seedling emergence under field conditions in two successive years. Across the three populations, BC(2)F(1) families segregating for the donor QTL allele linked to the marker umc139 (on chromosome 2), bnl9.08 (on chromosome 8), or php200689 (on chromosome 1) displayed 40.8, 30.2, and 28.2% increases in seedling emergence, respectively, over the unmodified F(1)s. The introgressed QTL alleles were observed to enhance seedling emergence in the BC(2)F(1) generation as was observed in the original F(2:3) mapping population. Marker-QTL associated effects were reproducible across generations and populations indicating that QTL identified in one population can exert similar effects in different genetic backgrounds. Results suggest that using DNA marker technology can help to identify and introgress beneficial QTL alleles, shortening the time and resources required to develop improved germplasm.
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Claims 7
Claim · model-written summary
Three RFLP marker alleles linked to QTL enhancing seedling emergence were identified in an F(2:3) sweet corn mapping population.
- Taxon
- sweet corne0
- Outcome
- RFLP marker alleles linked to QTL that enhanced seedling emergencee0
- Value
- Threee0
Not reportedDirection
Verbatim
e0“Three RFLP marker alleles linked to QTL that enhanced seedling emergence were identified in an F(2:3) sweet corn mapping population”
Claim · model-written summary
BC2F1 families segregating for the donor QTL allele linked to umc139 (chromosome 2) showed a 40.8% increase in seedling emergence over unmodified F1s.
Verbatim
e0“Three RFLP marker alleles linked to QTL that enhanced seedling emergence were identified in an F(2:3) sweet corn mapping population”
e1“families segregating for the donor QTL allele linked to the marker umc139 (on chromosome 2), bnl9.08 (on chromosome 8), or php200689 (on chromosome 1) displayed 40.8, 30.2, and 28.2% increases in seedling emergence, respectively, over the unmodified F(1)s”
Claim · model-written summary
BC2F1 families segregating for the donor QTL allele linked to bnl9.08 (chromosome 8) showed a 30.2% increase in seedling emergence over unmodified F1s.
Verbatim
e0“Three RFLP marker alleles linked to QTL that enhanced seedling emergence were identified in an F(2:3) sweet corn mapping population”
e1“families segregating for the donor QTL allele linked to the marker umc139 (on chromosome 2), bnl9.08 (on chromosome 8), or php200689 (on chromosome 1) displayed 40.8, 30.2, and 28.2% increases in seedling emergence, respectively, over the unmodified F(1)s”
Claim · model-written summary
BC2F1 families segregating for the donor QTL allele linked to php200689 (chromosome 1) showed a 28.2% increase in seedling emergence over unmodified F1s.
Verbatim
e0“Three RFLP marker alleles linked to QTL that enhanced seedling emergence were identified in an F(2:3) sweet corn mapping population”
e1“families segregating for the donor QTL allele linked to the marker umc139 (on chromosome 2), bnl9.08 (on chromosome 8), or php200689 (on chromosome 1) displayed 40.8, 30.2, and 28.2% increases in seedling emergence, respectively, over the unmodified F(1)s”
Claim · model-written summary
The introgressed QTL alleles enhanced seedling emergence in the BC2F1 generation as observed in the original F2:3 mapping population.
Not reportedComparator
Verbatim
e0“Three RFLP marker alleles linked to QTL that enhanced seedling emergence were identified in an F(2:3) sweet corn mapping population”
e1“The introgressed QTL alleles were observed to enhance seedling emergence in the BC(2)F(1) generation as was observed in the original F(2:3) mapping population”
Claim · model-written summary
Marker-QTL associated effects were reproducible across generations and populations, indicating that a QTL identified in one population can exert similar effects in different genetic backgrounds.
- Taxon
- sweet corne0
- Outcome
- Marker-QTL associated effects were reproducible across generations and populationse1
Not reportedDirection
Verbatim
e0“Three RFLP marker alleles linked to QTL that enhanced seedling emergence were identified in an F(2:3) sweet corn mapping population”
e1“Marker-QTL associated effects were reproducible across generations and populations indicating that QTL identified in one population can exert similar effects in different genetic backgrounds”
Claim · model-written summary
The authors conclude that DNA marker technology can help identify and introgress beneficial QTL alleles, shortening the time and resources needed to develop improved germplasm.
Verbatim
e0“Three RFLP marker alleles linked to QTL that enhanced seedling emergence were identified in an F(2:3) sweet corn mapping population”
e1“using DNA marker technology can help to identify and introgress beneficial QTL alleles, shortening the time and resources required to develop improved germplasm”
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