Abstract
The growth experiments in which plant cell suspensions of N. tabacum 1507 and L. vera MM were cultivated in two-phase systems were carried out in order to select the best phase formulation ensuring culture growth. The highest growth observed for N. tabacum 1507 occurred in 5% PEG 6000/10% dextran 70 000. The overproduction of extracell phenolic compounds from both cell cultures in the formulated two-phase systems was investigated. The best two-phase system ensuring higher production of phenolic compounds from both cell cultures is 4% PEG 20 000/7.5% dextran 70 000. The plant cell suspensions of N. tabacum 1507 produced 0.171 g dm−3 total phenolic compounds in this system (against 0.028 g dm−3 from control fermentation). In the same two-phase system, the plant cell suspension from L. vera MM produced 0.387 g dm−3 total phenolic compounds (against 0.058 g dm−3 from control fermentation). In both cases the phenolic partitioned mainly in the top phase.
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Claims 5
Claim · model-written summary
N. tabacum growth was highest in 5% PEG 6000/10% dextran 70 000.
- Taxon
- N. tabacum 1507
Nicotiana tabacume0 - Treatment
- 5% PEG 6000/10% dextran 70 000e0
- Outcome
- growthe0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana Tabacum 1507 and Lavandula Vera mm”
e0“The highest growth observed for N. tabacum 1507 occurred in 5% PEG 6000/10% dextran 70 000”
Claim · model-written summary
The 4% PEG 20 000/7.5% dextran system gave higher phenolic production in both cultures.
- Taxon
- Nicotiana Tabacum 1507 and Lavandula Vera mmt0
- Treatment
- 4% PEG 20 000/7.5% dextran 70 000e0
- Outcome
- production of phenolic compoundse0
- Direction
- highere0
Not reportedComparator
Verbatim
t0“Nicotiana Tabacum 1507 and Lavandula Vera mm”
e0“The best two-phase system ensuring higher production of phenolic compounds from both cell cultures is 4% PEG 20 000/7.5% dextran 70 000”
Claim · model-written summary
N. tabacum produced 0.171 g dm−3 phenolics versus 0.028 in control.
- Taxon
- N. tabacum 1507Nicotiana tabacume0
- Treatment
- this systeme0
- Outcome
- total phenolic compoundse0
- Direction
- higherinferrede0
- Value
- 0.171 g dm−3e0
- Qualifier
- 0.028 g dm−3e0
- Comparator
- control fermentatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana Tabacum 1507 and Lavandula Vera mm”
e0“The plant cell suspensions of N. tabacum 1507 produced 0.171 g dm−3 total phenolic compounds in this system (against 0.028 g dm−3 from control fermentation)”
Claim · model-written summary
L. vera produced 0.387 g dm−3 phenolics versus 0.058 in control.
Verbatim
t0“Nicotiana Tabacum 1507 and Lavandula Vera mm”
e0“the plant cell suspension from L. vera MM produced 0.387 g dm−3 total phenolic compounds (against 0.058 g dm−3 from control fermentation)”
e1“The best two-phase system ensuring higher production of phenolic compounds from both cell cultures is 4% PEG 20 000/7.5% dextran 70 000”
Claim · model-written summary
Phenolics partitioned mainly into the top phase in both cultures.
Verbatim
t0“Nicotiana Tabacum 1507 and Lavandula Vera mm”
e0“In both cases the phenolic partitioned mainly in the top phase”
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