Abstract
To standardize a kernel wound inoculation technique for evaluation of maize genotypes for resistance to gibberella ear rot, four parameters associated with its application were investigated: inoculation position, time of inoculation after silk emergence, macroconidial concentration, and irrigation. Two moderately resistant (Pride K127 and Funk G4106) and two susceptible (Pioneer 3953 and Dekalb DK-415) hybrids were inoculated through the husk by wounding 3-4 kernels in the centre of the ear with four stainless steel pins that had previously been dipped in a macroconidial suspension of Fusarium graminearum. Disease severity was determined at harvest. In four experiments conducted in 1993 and 1994, ears were inoculated using three inoculation positions (tip, centre, and butt of the ear); seven conidial suspension concentrations ranging from 0 to 2 X 10(6) conidia/mL; 12 (1993) and 9 (1994) different times of inoculation, ranging from 0 to 44 days post-silk emergence; and wounding and a silk channel injection techniques under irrigated and nonirrigated conditions. Disease severity was highest with inoculations made in the centre of the ear. Disease severity increased with increasing conidial concentrations but decreased with increasing kernel/silk age. Irrigation tended to lower disease severity and did not significantly enhance differentiation between genotypes. Best differentiation between genotypes was obtained when inoculations were made in the centre of the ear at approximately 15 days post-silk emergence and with a suspension of at least 10(5) conidia/mL suspension.
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Claims 6
Claim · model-written summary
Centre ear inoculation produced the highest disease severity.
- Treatment
- inoculations made in the centre of the eare0
- Interactor
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearumt0pathogen - Outcome
- Disease severitye0
- Direction
- higheste0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“Disease severity was highest with inoculations made in the centre of the ear.”
t0“maize to Fusarium graminearum”
Claim · model-written summary
Disease severity rose with conidial concentration.
- Treatment
- conidial concentrationse0
- Interactor
- Fusarium graminearumFusarium graminearumt0pathogen
- Outcome
- Disease severitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disease severity increased with increasing conidial concentrations”
t0“maize to Fusarium graminearum”
Claim · model-written summary
Disease severity fell as kernel or silk age rose.
- Organ / stage
- kernel/silke0
- Interactor
- Fusarium graminearumFusarium graminearumt0pathogen
- Outcome
- Disease severitye0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disease severity increased with increasing conidial concentrations but decreased with increasing kernel/silk age.”
t0“maize to Fusarium graminearum”
Claim · model-written summary
Irrigation tended to lower disease severity.
- Treatment
- Irrigatione0
- Interactor
- Fusarium graminearumFusarium graminearumt0pathogen
- Outcome
- disease severitye0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Irrigation tended to lower disease severity”
t0“maize to Fusarium graminearum”
Claim · model-written summary
Irrigation did not significantly enhance genotype differentiation.
- Treatment
- Irrigationt1
- Outcome
- differentiation between genotypese0
- Direction
- did not significantly enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did not significantly enhance differentiation between genotypes”
t0“maize to Fusarium graminearum”
t1“Irrigation tended to lower disease severity and did not significantly enhance differentiation between genotypes.”
Claim · model-written summary
Best genotype differentiation occurred with centre ear inoculation around 15 days post silk emergence and at least 10(5) conidia/mL.
- Treatment
- inoculations were made in the centre of the eare0
- Outcome
- Best differentiation between genotypese0
- Value
- at least 10(5) conidia/mLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Best differentiation between genotypes was obtained when inoculations were made in the centre of the ear at approximately 15 days post-silk emergence and with a suspension of at least 10(5) conidia/mL suspension.”
t0“maize to Fusarium graminearum”
The same questions in other papers
inoculation · Fusarium graminearum · Zea mays (maize)Positive and negative results
4 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.