Abstract
The germination of lentil seeds was gradually reduced when seeds were exposed to temperature of 30 or 40 °C, either alone or combined with 0.1, 0.2 or 0.3 M NaCl or 34.1 % (m/v) PEG 8000, during 6 -12 h imbibition. [35S]-methionine incorporation in 12 h imbibed lentil axes also decreased with increasing NaCl concentration at 20 and 40 °C, whereas at 30 °C only 0.3 M NaCl treatment partially inhibited protein synthesis. An analysis of newly synthesized proteins by 1-D SDS PAGE, showed that the expression of most polypeptides decreased following increasing stress. Among these, low molecular mass heat-shock proteins declining, higher in 40 °C treated axes than those treated at 30 °C, supports the hypothesis that at this temperature maximal level of expression of these proteins was achieved.
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Claims 5
Claim · model-written summary
Germination of lentil seeds was gradually reduced by exposure to 30 or 40 degrees, alone or combined with NaCl or PEG.
- Taxon
- lentil
Lens culinarise1 - Treatment
- temperature of 30 or 40e1
- Outcome
- germinatione1
- Direction
- gradually reducede1
- Qualifier
- combined with 0.1, 0.2 or 0.3 M NaCl or 34.1 % (m/v) PEG 8000e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The germination of lentil seeds was gradually reduced when seeds were exposed to temperature of 30 or 40”
e2“combined with 0.1, 0.2 or 0.3 M NaCl or 34.1 % (m/v) PEG 8000”
Claim · model-written summary
[35S]-methionine incorporation in lentil axes decreased with increasing NaCl concentration at 20 and 40 degrees.
- Taxon
- lentilLens culinarise1
- Organ / stage
- axese1
- Treatment
- increasing NaCl concentratione1
- Outcome
- [35S]-methionine incorporatione1
- Direction
- decreasede1
- Qualifier
- at 20 and 40e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“[35S]-methionine incorporation in 12 h imbibed lentil axes also decreased with increasing NaCl concentration at 20 and 40”
Claim · model-written summary
Only 0.3 M NaCl partially inhibited protein synthesis in lentil axes at 30 degrees.
- Taxon
- lentilLens culinarise0
- Organ / stage
- axese0
- Treatment
- 0.3 M NaCle3
- Outcome
- protein synthesise3
- Direction
- partially inhibitede3
- Qualifier
- at 30e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“12 h imbibed lentil axes”
e3“only 0.3 M NaCl treatment partially inhibited protein synthesis”
e4“whereas at 30”
Claim · model-written summary
Low molecular mass heat-shock proteins declined under combined salt and heat stress, and were higher at 40 than at 30 degrees.
- Taxon
- lentilLens culinarise0
- Treatment
- Combined Salt and Heat Treatmentst0
- Outcome
- low molecular mass heat-shock proteinse5
- Direction
- declininge5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“12 h imbibed lentil axes”
e5“low molecular mass heat-shock proteins declining, higher in 40”
t0“Combined Salt and Heat Treatments”
Claim · model-written summary
The expression of most polypeptides in lentil decreased with increasing stress.
- Taxon
- lentilLens culinarise0
- Treatment
- increasing stresse6
- Outcome
- expression of most polypeptidese6
- Direction
- decreasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“12 h imbibed lentil axes”
e6“the expression of most polypeptides decreased following increasing stress”
The same questions in other papers
sodium chloride · molecular: metabolites · Lens culinaris (lentil)Results agree
3 papers in the same direction as this paper
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.