Paper · 1983 · Annals of Applied Biology

Double resistance by citrus green mould Penicillium digitatum to the fungicides guazatine and benomyl

B. L. WILD

6
claims
12
verbatim quotes
26
citations
2
research areas
—
Not open access

Abstract

SUMMARY In vitro dosage response data with different isolates of Penicillium digitatum and the fungicide guazatine indicated an approximate 10‐fold shift in tolerance when compared with wild type strains. ED 50 values for resistant strains were approximately 0.5 μg/ml compared to approximately 0.05, μg/ml for the wild type strains. Colony growth of guazatine resistant isolates on selective media containing carbendazim showed that they were also resistant to the benzimidazole group of fungicides. In vivo tests in inoculated oranges with strains previously characterised by in vitro tests confirmed resistance to guazatine and benomyl. A combined treatment of these fungicides at 400 /μ/ml and 500 μg/ml respectively, which normally gives protection against decay, also failed to provide adequate mould control. Growth and pathogenicity of the resistant strains in these tests in oranges were indistinguishable from that of wild type strains.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Penicillium digitatum resistant isolates had approximately tenfold higher guazatine tolerance than wild type strains in vitro.

Taxon
Penicillium digitatumPenicillium digitatume0
Outcome
tolerancee0
Direction
shifte0
Value
approximate 10‐folde0
Comparator
wild type strainse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Penicillium digitatum
Penicillium digitatum · TaxonKeith Seifert · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In vitro dosage response data with different isolates of Penicillium digitatum and the fungicide guazatine indicated an approximate 10‐fold shift in tolerance when compared with wild type strains.”

✓ located verbatim in the source · abstract, characters 8–204 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Guazatine ED50 was about 0.5 μg/ml for resistant strains and 0.05 μg/ml for wild type strains.

Taxon
Penicillium digitatumPenicillium digitatumt0
Outcome
ED 50 valuese0
Value
0.5 μg/mle0
Qualifier
0.05, μg/mle0
Comparator
wild type strainse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Penicillium digitatum
Penicillium digitatum · TaxonKeith Seifert · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“ED 50 values for resistant strains were approximately 0.5 μg/ml compared to approximately 0.05, μg/ml for the wild type strains.”

✓ located verbatim in the source · abstract, characters 205–333 · show in text ↑
t0

“Penicillium digitatum”

✓ located verbatim in the source · title, characters 40–61 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Guazatine-resistant isolates were also resistant to benzimidazole fungicides.

Taxon
Penicillium digitatumPenicillium digitatumt0
Outcome
resistant to the benzimidazole group of fungicidese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Penicillium digitatum
Penicillium digitatum · TaxonKeith Seifert · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Colony growth of guazatine resistant isolates on selective media containing carbendazim showed that they were also resistant to the benzimidazole group of fungicides.”

✓ located verbatim in the source · abstract, characters 334–500 · show in text ↑
t0

“Penicillium digitatum”

✓ located verbatim in the source · title, characters 40–61 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Inoculated oranges confirmed resistance of Penicillium strains to guazatine and benomyl.

Taxon
orangese0
Interactor
Penicillium digitatumPenicillium digitatumt0pathogen
Outcome
resistance to guazatine and benomyle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Penicillium digitatum
Penicillium digitatum · InteractorKeith Seifert · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In vivo tests in inoculated oranges with strains previously characterised by in vitro tests confirmed resistance to guazatine and benomyl.”

✓ located verbatim in the source · abstract, characters 501–639 · show in text ↑
t0

“Penicillium digitatum”

✓ located verbatim in the source · title, characters 40–61 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Combined guazatine and benomyl treatment failed to control mould on oranges adequately.

Taxon
citrusCitrust0
Treatment
combined treatment of these fungicides at 400 /μ/ml and 500 μg/ml respectivelye0
Interactor
Penicillium digitatumPenicillium digitatume1pathogen
Outcome
mould controle0
Direction
failed to provide adequatee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Citrus
Citrus · Taxon · picture of the genus (one of its species)Ellen Levy Finch ( Elf ) · CC BY-SA 3.0 · Wikimedia Commons ↗
Penicillium digitatum
Penicillium digitatum · InteractorKeith Seifert · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A combined treatment of these fungicides at 400 /μ/ml and 500 μg/ml respectively, which normally gives protection against decay, also failed to provide adequate mould control.”

✓ located verbatim in the source · abstract, characters 640–815 · show in text ↑
t0

“citrus green mould Penicillium digitatum”

✓ located verbatim in the source · title, characters 21–61 · show in text ↑
e1

“In vitro dosage response data with different isolates of Penicillium digitatum and the fungicide guazatine indicated an approximate 10‐fold shift in tolerance when compared with wild type strains.”

✓ located verbatim in the source · abstract, characters 8–204 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Resistant and wild type strains showed indistinguishable growth and pathogenicity in oranges.

Taxon
orangese0
Interactor
Penicillium digitatumPenicillium digitatumt0pathogen
Outcome
Growth and pathogenicity of the resistant strainse0
Direction
indistinguishablee0
Comparator
wild type strainse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Penicillium digitatum
Penicillium digitatum · InteractorKeith Seifert · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Growth and pathogenicity of the resistant strains in these tests in oranges were indistinguishable from that of wild type strains.”

✓ located verbatim in the source · abstract, characters 816–946 · show in text ↑
t0

“Penicillium digitatum”

✓ located verbatim in the source · title, characters 40–61 · show in text ↑

fungicide · Penicillium digitatum · CitrusPositive and negative results

5 papers: 3 positive, 2 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.