Abstract
The effect was studied of chloride ions, added in the form of different salts, on nitrate reductase (NR) level in excised pea roots, on anaerobic nitrite production in an assay medium lacking both nitrate and n-propanol, on nitrate content in the roots, and on in vivo NR activity determined in an assay medium containing 5% n-propanol. The presence of Cl in nitrate containing nutrient solutions resulted in lower NR levels, however counterions supplied together with Cl tended to modify slightly this general trend. The negative effect of Cl ions was also apparent, when Cl ions were applied before nitrate ions. Anaerobic nitrite production in the medium lacking both nitrate and n-propanol was not influenced by chloride ions. Nitrate content in the roots was reduced in the presence of chloride both at 3 mM and 15 mM NO3 in nutrient solutions; however, at 16 mM NO3, nitrate content in the roots exoeeded even in the presence of 15 mM Cl nitrate content in those root segments which were cultivated in a nutrient solution with 6 mM nitrate, which is the concentration at which NR reaches the level of saturation in excised pea roots. The results obtained suggest that a special induction nitrate pool exists in plant cells besides the storage and metabolic nitrate pools.
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Claims 8
Claim · model-written summary
Chloride in nitrate nutrient solution lowered nitrate reductase levels in pea roots.
- Taxon
- Pisum sativum L.
Lathyrus oleraceust0 - Treatment
- Cle0
- Outcome
- NR levelse0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The presence of Cl in nitrate containing nutrient solutions resulted in lower NR levels”
t0“Pisum sativum L.”
Claim · model-written summary
Counterions slightly modified the chloride effect on nitrate reductase levels.
- Taxon
- Pisum sativum L.
Lathyrus oleraceust0 - Treatment
- counterions supplied together with Cle0
- Outcome
- modify slightly this general trende0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“counterions supplied together with Cl tended to modify slightly this general trend”
t0“Pisum sativum L.”
Claim · model-written summary
Applying chloride before nitrate also negatively affected nitrate reductase.
- Taxon
- Pisum sativum L.
Lathyrus oleraceust0 - Treatment
- Cl ionse0
- Outcome
- negative effecte0
- Direction
- negativee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The negative effect of Cl ions was also apparent, when Cl ions were applied before nitrate ions”
t0“Pisum sativum L.”
Claim · model-written summary
Chloride did not influence anaerobic nitrite production in nitrate- and n-propanol-free medium.
- Taxon
- Pisum sativum L.
Lathyrus oleraceust0 - Treatment
- chloride ionse0
- Outcome
- Anaerobic nitrite productione0
- Direction
- not influencede0
- Qualifier
- medium lacking both nitrate and n-propanole0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Anaerobic nitrite production in the medium lacking both nitrate and n-propanol was not influenced by chloride ions”
t0“Pisum sativum L.”
Claim · model-written summary
Chloride reduced root nitrate content at 3 mM nitrate in nutrient solution.
- Taxon
- Pisum sativum L.
Lathyrus oleraceust0 - Treatment
- chloridee0
- Outcome
- Nitrate content in the rootse0
- Direction
- reducede0
- Qualifier
- 3 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Nitrate content in the roots was reduced in the presence of chloride both at 3 mM and 15 mM NO3 in nutrient solutions”
t0“Pisum sativum L.”
Claim · model-written summary
Chloride reduced root nitrate content at 15 mM nitrate in nutrient solution.
- Taxon
- Pisum sativum L.
Lathyrus oleraceust0 - Treatment
- chloridee0
- Outcome
- Nitrate content in the rootse0
- Direction
- reducede0
- Qualifier
- 15 mM NO3e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Nitrate content in the roots was reduced in the presence of chloride both at 3 mM and 15 mM NO3 in nutrient solutions”
t0“Pisum sativum L.”
Claim · model-written summary
At 16 mM nitrate, root nitrate content with 15 mM chloride exceeded content at 6 mM nitrate.
- Taxon
- Pisum sativum L.
Lathyrus oleraceust0 - Treatment
- 16 mM NO3e0
- Outcome
- nitrate content in the rootse0
- Direction
- exoeedede0
- Qualifier
- 15 mM Cle0
- Comparator
- 6 mM nitratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“at 16 mM NO3, nitrate content in the roots exoeeded even in the presence of 15 mM Cl nitrate content in those root segments which were cultivated in a nutrient solution with 6 mM nitrate”
t0“Pisum sativum L.”
Claim · model-written summary
The results suggest a distinct induction nitrate pool in plant cells.
- Taxon
- Pisum sativum L.
Lathyrus oleraceust0 - Outcome
- special induction nitrate pool exists in plant cellse0
- Qualifier
- besides the storage and metabolic nitrate poolse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The results obtained suggest that a special induction nitrate pool exists in plant cells besides the storage and metabolic nitrate pools”
t0“Pisum sativum L.”
The same questions in other papers
chloride · yield · Lathyrus oleraceus (pea)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.