Paper · 1978 · Biologia Plantarum

The effect of chloride on nitrate reductase level, on anaerobic nitrite production, and on nitrate content in excisedPisum sativum L. roots

J. Sahulka

8
claims
16
verbatim quotes
6
citations
1
research areas
—
Not open access

Abstract

The effect was studied of chloride ions, added in the form of different salts, on nitrate reductase (NR) level in excised pea roots, on anaerobic nitrite production in an assay medium lacking both nitrate and n-propanol, on nitrate content in the roots, and on in vivo NR activity determined in an assay medium containing 5% n-propanol. The presence of Cl in nitrate containing nutrient solutions resulted in lower NR levels, however counterions supplied together with Cl tended to modify slightly this general trend. The negative effect of Cl ions was also apparent, when Cl ions were applied before nitrate ions. Anaerobic nitrite production in the medium lacking both nitrate and n-propanol was not influenced by chloride ions. Nitrate content in the roots was reduced in the presence of chloride both at 3 mM and 15 mM NO3 in nutrient solutions; however, at 16 mM NO3, nitrate content in the roots exoeeded even in the presence of 15 mM Cl nitrate content in those root segments which were cultivated in a nutrient solution with 6 mM nitrate, which is the concentration at which NR reaches the level of saturation in excised pea roots. The results obtained suggest that a special induction nitrate pool exists in plant cells besides the storage and metabolic nitrate pools.

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Claims 8

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chloride in nitrate nutrient solution lowered nitrate reductase levels in pea roots.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Treatment
Cle0
Outcome
NR levelse0
Direction
lowere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The presence of Cl in nitrate containing nutrient solutions resulted in lower NR levels”

✓ located verbatim in the source · abstract, characters 337–424 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 117–133 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Counterions slightly modified the chloride effect on nitrate reductase levels.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Treatment
counterions supplied together with Cle0
Outcome
modify slightly this general trende0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“counterions supplied together with Cl tended to modify slightly this general trend”

✓ located verbatim in the source · abstract, characters 434–516 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 117–133 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Applying chloride before nitrate also negatively affected nitrate reductase.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Treatment
Cl ionse0
Outcome
negative effecte0
Direction
negativee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The negative effect of Cl ions was also apparent, when Cl ions were applied before nitrate ions”

✓ located verbatim in the source · abstract, characters 518–613 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 117–133 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Chloride did not influence anaerobic nitrite production in nitrate- and n-propanol-free medium.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Treatment
chloride ionse0
Outcome
Anaerobic nitrite productione0
Direction
not influencede0
Qualifier
medium lacking both nitrate and n-propanole0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Anaerobic nitrite production in the medium lacking both nitrate and n-propanol was not influenced by chloride ions”

✓ located verbatim in the source · abstract, characters 615–729 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 117–133 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chloride reduced root nitrate content at 3 mM nitrate in nutrient solution.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Treatment
chloridee0
Outcome
Nitrate content in the rootse0
Direction
reducede0
Qualifier
3 mMe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Nitrate content in the roots was reduced in the presence of chloride both at 3 mM and 15 mM NO3 in nutrient solutions”

✓ located verbatim in the source · abstract, characters 731–848 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 117–133 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chloride reduced root nitrate content at 15 mM nitrate in nutrient solution.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Treatment
chloridee0
Outcome
Nitrate content in the rootse0
Direction
reducede0
Qualifier
15 mM NO3e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Nitrate content in the roots was reduced in the presence of chloride both at 3 mM and 15 mM NO3 in nutrient solutions”

✓ located verbatim in the source · abstract, characters 731–848 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 117–133 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

At 16 mM nitrate, root nitrate content with 15 mM chloride exceeded content at 6 mM nitrate.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Treatment
16 mM NO3e0
Outcome
nitrate content in the rootse0
Direction
exoeedede0
Qualifier
15 mM Cle0
Comparator
6 mM nitratee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“at 16 mM NO3, nitrate content in the roots exoeeded even in the presence of 15 mM Cl nitrate content in those root segments which were cultivated in a nutrient solution with 6 mM nitrate”

✓ located verbatim in the source · abstract, characters 859–1045 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 117–133 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The results suggest a distinct induction nitrate pool in plant cells.

Taxon
Pisum sativum L.Lathyrus oleraceust0
Outcome
special induction nitrate pool exists in plant cellse0
Qualifier
besides the storage and metabolic nitrate poolse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The results obtained suggest that a special induction nitrate pool exists in plant cells besides the storage and metabolic nitrate pools”

✓ located verbatim in the source · abstract, characters 1140–1276 · show in text ↑
t0

“Pisum sativum L.”

✓ located verbatim in the source · title, characters 117–133 · show in text ↑

chloride · yield · Lathyrus oleraceus (pea)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.