Abstract
DNAs complementary to a size-selected fraction of poly(A)(+) RNA present in elicitor-treated cells of bean (Phaseolus vulgaris L.) were inserted into pAT153 and used to transform Escherichia coli strain C600. Five clones were identified by hybrid-selected translation and cross-hybridization that contained sequences complementary to mRNA encoding phenylalanine ammonia-lyase (EC 4.3.1.5), which catalyzes the first reaction of phenylpropanoid biosynthesis. The longest insert contained a single open reading frame of 1520 base pairs together with 223 base pairs of 3' untranslated sequence. RNA blot hybridization showed that elicitor caused a rapid, marked but transient increase in phenylalanine ammonia-lyase mRNA that was closely correlated with changes in translatable mRNA activity in vitro and enzyme synthesis in vivo. Blot hybridization of newly synthesized mRNA purified by organomercurial affinity chromatography following in vivo pulse-labeling with 4-thiouridine indicates that elicitor caused a rapid stimulation of phenylalanine ammonia-lyase mRNA synthesis as an early in the defense response leading to accumulation of phenylpropanoid-derived phytoalexins.
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Claims 6
Claim · model-written summary
Elicitor caused a rapid, marked but transient increase in phenylalanine ammonia-lyase mRNA in bean cells.
- Taxon
- bean (Phaseolus vulgaris L.)
Phaseolus vulgarise0 - Treatment
- elicitore1
- Gene
- phenylalanine ammonia-lyasee1
- Outcome
- phenylalanine ammonia-lyase mRNAe1
- Direction
- increasee1
- Qualifier
- transiente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elicitor-treated cells of bean (Phaseolus vulgaris L.)”
e1“elicitor caused a rapid, marked but transient increase in phenylalanine ammonia-lyase mRNA”
Claim · model-written summary
The increase in phenylalanine ammonia-lyase mRNA is closely correlated with changes in translatable mRNA activity in vitro and enzyme synthesis in vivo.
- Taxon
- bean (Phaseolus vulgaris L.)Phaseolus vulgarise0
- Outcome
- translatable mRNA activitye2
- Setting
- in vivoe2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elicitor-treated cells of bean (Phaseolus vulgaris L.)”
e2“that was closely correlated with changes in translatable mRNA activity in vitro and enzyme synthesis in vivo”
Claim · model-written summary
Elicitor caused a rapid stimulation of phenylalanine ammonia-lyase mRNA synthesis.
- Taxon
- bean (Phaseolus vulgaris L.)Phaseolus vulgarise0
- Treatment
- elicitore3
- Gene
- phenylalanine ammonia-lyasee3
- Outcome
- phenylalanine ammonia-lyase mRNA synthesise3
- Direction
- stimulatione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elicitor-treated cells of bean (Phaseolus vulgaris L.)”
e3“elicitor caused a rapid stimulation of phenylalanine ammonia-lyase mRNA synthesis”
Claim · model-written summary
The longest cDNA insert contains a single open reading frame of 1520 base pairs.
- Taxon
- bean (Phaseolus vulgaris L.)Phaseolus vulgarise0
- Outcome
- single open reading framee4
- Value
- 1520 base pairse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elicitor-treated cells of bean (Phaseolus vulgaris L.)”
e4“contained a single open reading frame of 1520 base pairs”
Claim · model-written summary
The longest cDNA insert has 223 base pairs of 3' untranslated sequence.
- Taxon
- bean (Phaseolus vulgaris L.)Phaseolus vulgarise0
- Outcome
- 3' untranslated sequencee5
- Value
- 223 base pairse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elicitor-treated cells of bean (Phaseolus vulgaris L.)”
e5“223 base pairs of 3' untranslated sequence”
Claim · model-written summary
Five clones contain sequences complementary to mRNA encoding phenylalanine ammonia-lyase.
- Taxon
- bean (Phaseolus vulgaris L.)Phaseolus vulgarise0
- Gene
- phenylalanine ammonia-lyasee6
- Outcome
- sequences complementary to mRNA encoding phenylalanine ammonia-lyasee6
- Value
- Five clonese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elicitor-treated cells of bean (Phaseolus vulgaris L.)”
e6“Five clones were identified by hybrid-selected translation and cross-hybridization that contained sequences complementary to mRNA encoding phenylalanine ammonia-lyase”
The same questions in other papers
resistance inducer · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.