Abstract
A mutation in an inbred line of petunia (Petunia hybrida) produces a reduction in the deep-purple corolla pigmentation and changes the anther color from yellow to white. In addition, the mutant, designated white anther (wha), is functionally male sterile. The inability of pollen from wha plants to germinate in vitro provides a physiological basis for the lack of seed set observed in self-crosses of the mutant. Biochemical complementation with nanomolar amounts of kaempferol, a flavonol aglycone, confirms that the inability of the wha pollen to germinate is due to a lack of this essential compound. Transgenic complementation with a functional ChsA (Chalcone synthase A) cDNA suggests that the genetic lesion responsible for the wha phenotype is in Chs, the gene for the first enzyme in the flavonol biosynthesis pathway. The genetic background of the parental line, as well as the pollen phenotype, allowed us to deduce that the wha mutation is in ChsA. To our knowledge, wha is the first induced, nontransgenic Chs mutant described in petunia, and analysis of the mutation confirms earlier molecular and genetic observations that only two Chs genes (A and J) are expressed in reproductive tissues and that they are differentially regulated in corolla and anther.
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Claims 10
Claim · model-written summary
The mutation reduced deep-purple corolla pigmentation.
_flowers_in_Westgate_Gardens%2C_Canterbury.jpg?width=60){kind=small}
PetuniaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
_flowers_in_Westgate_Gardens%2C_Canterbury.jpg?width=500)
Verbatim
e0“A mutation in an inbred line of petunia (Petunia hybrida) produces a reduction in the deep-purple corolla pigmentation”
Claim · model-written summary
The mutation changed anther color from yellow to white.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“changes the anther color from yellow to white”
t0“Petunia Mutant”
Claim · model-written summary
The white anther mutant was functionally male sterile.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutant, designated white anther (wha), is functionally male sterile”
t0“Petunia Mutant”
Claim · model-written summary
Pollen from wha plants failed to germinate in vitro.
Not reportedDirection
Verbatim
e0“The inability of pollen from wha plants to germinate in vitro”
Claim · model-written summary
Self-crosses of the mutant lacked seed set.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lack of seed set observed in self-crosses of the mutant”
t0“Petunia Mutant”
Claim · model-written summary
Nanomolar kaempferol complemented the pollen germination defect.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Biochemical complementation with nanomolar amounts of kaempferol, a flavonol aglycone, confirms that the inability of the wha pollen to germinate is due to a lack of this essential compound”
t0“Petunia Mutant”
Claim · model-written summary
Complementation by ChsA cDNA implicated Chs in the wha phenotype.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic complementation with a functional ChsA (Chalcone synthase A) cDNA suggests that the genetic lesion responsible for the wha phenotype is in Chs”
t0“Petunia Mutant”
Claim · model-written summary
The wha mutation was deduced to be in ChsA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“allowed us to deduce that the wha mutation is in ChsA”
t0“Petunia Mutant”
Claim · model-written summary
Only two Chs genes, A and J, were expressed in reproductive tissues.
- Organ / stage
- reproductive tissuese0
- Gene
- Chs genes (A and J)e0
- Outcome
- expressed in reproductive tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only two Chs genes (A and J) are expressed in reproductive tissues”
t0“Petunia Mutant”
Claim · model-written summary
The two Chs genes were differentially regulated in corolla and anther.
- Organ / stage
- corolla and anthere0
- Gene
- Chs genese0
- Outcome
- differentially regulated in corolla and anthere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only two Chs genes (A and J) are expressed in reproductive tissues and that they are differentially regulated in corolla and anther”
t0“Petunia Mutant”
The same questions in other papers
nanoparticle · growth · Petunia (petunia)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.