Abstract
국화는 전 세계적으로 가장 대중적인 화훼류 중의 하나로써, 최근 새로운 국화 품종들이 돌연변이육종을 통해 개발되고 있다. 그러나 현재까지 하나의 국화 원품종에서 유래한 돌연변이 품종들 간의 조직배양 조건 차이에 대한 보고는 전무하다. 따라서 본 연구에서는 2개의 국화 화색 돌연변이 품종 'ARTI-purple'과 'ARTI-queen'의 효율적인 재분화 조건을 비교하기 위해 수행되었다. 실험재료로 꽃봉오리 시기와 통상화 개화 이전 시기의 꽃잎을 이용하였고, 꽃의 조직에 따른 차이를 확인하기 위해 통상화 및 설상화를 구분하여 실험을 수행하였다. 국화 재분화를 위한 식물생장호르몬의 적정 조합을 찾기 위해 3% sucrose, 0.3% gelrite를 포함한 MS 배지에 BA, NAA, IAA 중 2가지 호르몬을 조합별로 첨가하여 실험을 수행하였다. 절편체는 일장 16시간, 온도 $25{\pm}1^{\circ}C$ 조건으로 배양하였으며, 재분화율 조사는 배양 후 4주 및 8주차에 실시하였다. 결과적으로, 가장 높은 재분화율은 두 품종 모두 최적 NAA와 BA의 호르몬 조합에서 꽃봉오리 시기의 통상화를 사용할 시 가장 높게 확인되었다. 재분화를 위한 식물생장호르몬의 최적 조합은 'ARTI-purple'의 경우 NAA $1.0mg{\cdot}L^{-1}$와 BA $0.5mg{\cdot}L^{-1}$로 47.9%의 재분화 효율을 보였으며, 'ARTI-queen'의 경우 NAA $2.0mg{\cdot}L^{-1}$와 BA $1.0mg{\cdot}L^{-1}$로 25.6%의 재분화 효율을 나타내었다. 재분화된 지상부는 1/2 MS배지에서 발근시켰으며, 기내 소식물체는 유리온실에서 성공적으로 순화되었다. 본 연구결과는 감마선을 사용한 돌연변이육종법에 의해 개발된 다양한 국화품종의 효율적인 재분화 시스템을 구축하는데 유용한 정보를 제공할 것이다. Chrysanthemum is one of the most popular ornamental plants worldwide. Recently, lots of new and novel chrysanthemum varieties have been developed using mutagenesis. However, there was no study for comparison of tissue culture condition among the mutant varieties derived from one original variety, until now. This study was conducted to compare the efficient regeneration condition of the two chrysanthemum mutant varieties, 'ARTI-purple' and 'ARTI-queen'. Two different flower parts (disk and ray florets) at the unopened and early blooming stages were used for comparison of regeneration condition on MS medium supplemented with combinations of three growth regulators (BA, NAA, and IAA). The highest regeneration rate was identified on the NAA and BA combination when the disk florets at unopened blooming stage are used. The best optimum combinations of growth regulators were identified as NAA $1.0mg{\cdot}L^{-1}$ and BA $0.5mg{\cdot}L^{-1}$ at 'ARTI-purple', which displayed 47.9% regeneration. However, regeneration of 'ARTI-queen' was the highest as 25.6% at NAA $2.0mg{\cdot}L^{-1}$ and BA $1.0mg{\cdot}L^{-1}$. There results indicate that there is a difference for the optimum regeneration condition between the mutant varieties derived from one original variety. These results will be useful for construction of efficient regeneration system of diverse chrysanthemum mutants developed by mutation breeding.
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Claims 4
Claim · model-written summary
Unopened disk florets on NAA and BA gave the highest chrysanthemum regeneration rate.
- Taxon
- Chrysanthemum
.jpeg?width=60)
Chrysanthemumt0 - Organ / stage
- disk florets at unopened blooming stagee0
- Treatment
- NAA and BA combinatione0
- Outcome
- regeneration ratee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“The highest regeneration rate was identified on the NAA and BA combination when the disk florets at unopened blooming stage are used.”
t0“Chrysanthemum”
Claim · model-written summary
ARTI-purple achieved 47.9% regeneration with NAA 1.0 mg/L and BA 0.5 mg/L.
- Taxon
- ChrysanthemumChrysanthemumt0
- Material
- ARTI-purplee0
- Treatment
- NAA $1.0mg{\cdot}L^{-1}$ and BA $0.5mg{\cdot}L^{-1}$e0
- Outcome
- regeneratione0
- Value
- 47.9%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best optimum combinations of growth regulators were identified as NAA $1.0mg{\cdot}L^{-1}$ and BA $0.5mg{\cdot}L^{-1}$ at 'ARTI-purple', which displayed 47.9% regeneration.”
t0“Chrysanthemum”
Claim · model-written summary
ARTI-queen achieved its highest regeneration, 25.6%, with NAA 2.0 mg/L and BA 1.0 mg/L.
- Taxon
- ChrysanthemumChrysanthemumt0
- Material
- ARTI-queene0
- Treatment
- NAA $2.0mg{\cdot}L^{-1}$ and BA $1.0mg{\cdot}L^{-1}$e0
- Outcome
- regeneratione0
- Direction
- higheste0
- Value
- 25.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“regeneration of 'ARTI-queen' was the highest as 25.6% at NAA $2.0mg{\cdot}L^{-1}$ and BA $1.0mg{\cdot}L^{-1}$”
t0“Chrysanthemum”
Claim · model-written summary
The two mutant varieties differ in their optimal regeneration conditions.
- Taxon
- ChrysanthemumChrysanthemumt0
- Material
- mutant varietiese0
- Outcome
- optimum regeneration conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there is a difference for the optimum regeneration condition between the mutant varieties derived from one original variety.”
t0“Chrysanthemum”
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