Abstract
Sugar beet protoplasts (Beta vulgaris L.) were isolated from hypocotyl-derived suspension cells and cultured on modified Murashige and Skoog medium supplemented with 5 μM naphthaleneacetic acid (NAA) and 2 μM 6-benzyl-aminopurine (BAP). Protoplasts were plated at a density 1.0-1.5×105 cm-3 and incubated in either liquid medium or in medium solidified by 1.2% agarose, at 25°C in the dark. Comparison of two methods of culture unequivocally showed the second to be superior. Immobilizing the protoplast in agarose proved to be essential for obtaining sustained protoplast division and reproducible colony formation. The plating efficiency after two weeks of culture, expressed as the percentage of protoplasts which developed to form colonies, reached 40%. Subsequent subcultures of protoplast-derived callus to regeneration media with different concentrations of BAP (5 μM, 10 μM, 20 μM, 30 μM) resulted in very good callus proliferation at the three lowest concentrations, although organogenesis was not achieved.
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Claims 5
Claim · model-written summary
Agarose-solidified culture was superior to liquid culture.
- Taxon
- Sugar beet
Beta vulgaris subsp. vulgarise1 - Organ / stage
- Protoplastse0
- Treatment
- medium solidified by 1.2% agarosee0
- Outcome
- superiore0
- Direction
- superiore0
- Comparator
- liquid mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protoplasts were plated at a density 1.0-1.5×105 cm-3 and incubated in either liquid medium or in medium solidified by 1.2% agarose, at 25°C in the dark. Comparison of two methods of culture unequivocally showed the second to be superior.”
e1“Sugar beet protoplasts (Beta vulgaris L.)”
Claim · model-written summary
Agarose immobilization was essential for sustained protoplast division and reproducible colony formation.
- Taxon
- Sugar beetBeta vulgaris subsp. vulgarise1
- Treatment
- Immobilizing the protoplast in agarosee0
- Outcome
- sustained protoplast division and reproducible colony formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immobilizing the protoplast in agarose proved to be essential for obtaining sustained protoplast division and reproducible colony formation.”
e1“Sugar beet protoplasts (Beta vulgaris L.)”
Claim · model-written summary
Protoplast colony plating efficiency reached 40% after two weeks of culture.
- Taxon
- Sugar beetBeta vulgaris subsp. vulgarise1
- Organ / stage
- protoplastse0
- Outcome
- plating efficiencye0
- Value
- 40%e0
- Qualifier
- after two weeks of culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plating efficiency after two weeks of culture, expressed as the percentage of protoplasts which developed to form colonies, reached 40%.”
e1“Sugar beet protoplasts (Beta vulgaris L.)”
Claim · model-written summary
Callus proliferated very well at BAP concentrations of 5, 10 and 20 μM.
- Taxon
- Sugar beetBeta vulgaris subsp. vulgarise1
- Organ / stage
- protoplast-derived calluse0
- Treatment
- BAP (5 μM, 10 μM, 20 μMe0
- Outcome
- callus proliferatione0
- Direction
- very goode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subsequent subcultures of protoplast-derived callus to regeneration media with different concentrations of BAP (5 μM, 10 μM, 20 μM, 30 μM) resulted in very good callus proliferation at the three lowest concentrations, although organogenesis was not achieved.”
e1“Sugar beet protoplasts (Beta vulgaris L.)”
Claim · model-written summary
Organogenesis was not achieved after callus subculture on BAP regeneration media.
- Taxon
- Sugar beetBeta vulgaris subsp. vulgarise1
- Treatment
- regeneration media with different concentrations of BAPe0
- Outcome
- organogenesis was not achievede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subsequent subcultures of protoplast-derived callus to regeneration media with different concentrations of BAP (5 μM, 10 μM, 20 μM, 30 μM) resulted in very good callus proliferation at the three lowest concentrations, although organogenesis was not achieved.”
e1“Sugar beet protoplasts (Beta vulgaris L.)”
The same questions in other papers
N-benzyladenine · growth · Beta vulgaris (beet)Results agree
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- positive
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.