Abstract
A 100% seed sterility and 75.6% seed germination rate were achieved when seeds of three sugar beet (Beta vulgaris L.) breeding lines were surface sterilized with a combination of 0.5% Domestos and 5% PPM™. As an in planta treatment with BAP, the sterilized seeds were germinated on MS basal medium containing 1, 3 or 5 mg/l BAP for five weeks and then petiole explants were excised from the seedlings and cultured on two different regeneration media; RGL medium containing 0.5 mg/l BAP and 0.1 mg/l NAA, and RGH medium containing 1.0 mg/l BAP and 0.5 mg/l NAA. Petiole explants taken from seedlings pretreated with 1 mg/l BAP produced significantly more shoots (0.97 shoot per explant) than those taken from seedlings pretreated with 3 or 5 mg/l BAP (0.68 and 0.59 shoot per explant, respectively). The RGL medium containing lower BAP and NAA was significantly more effective in direct shoot production than the RGH medium containing higher BAP and NAA, 1 shoot per explant compared to 0.48, respectively. 21.1% of the regenerated shoots developed roots on medium containing 3 mg/l NAA whereas only 4.1% of the shoots rooted on medium containing 1 mg/l NAA. Genotypic variation was significantly evident for both shoot regeneration and rooting.
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Claims 7
Claim · model-written summary
Combined Domestos and PPM sterilization achieved complete seed sterility.
- Taxon
- sugar beet
Beta vulgaris subsp. vulgarise0 - Treatment
- 0.5% Domestos and 5% PPM™e0
- Outcome
- seed sterilitye0
- Direction
- achievede0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 100% seed sterility and 75.6% seed germination rate were achieved when seeds of three sugar beet (Beta vulgaris L.) breeding lines were surface sterilized with a combination of 0.5% Domestos and 5% PPM™.”
Claim · model-written summary
Combined Domestos and PPM sterilization yielded 75.6 percent germination.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Treatment
- 0.5% Domestos and 5% PPM™e0
- Outcome
- seed germination ratee0
- Direction
- achievede0
- Value
- 75.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 100% seed sterility and 75.6% seed germination rate were achieved when seeds of three sugar beet (Beta vulgaris L.) breeding lines were surface sterilized with a combination of 0.5% Domestos and 5% PPM™.”
Claim · model-written summary
Pretreatment with 1 mg/l BAP produced more shoots per explant than 3 mg/l BAP.
- Organ / stage
- Petiole explantse0
- Treatment
- 1 mg/l BAPe0
- Outcome
- shootse0
- Direction
- moree0
- Value
- 0.97 shoot per explante0
- Comparator
- 3 or 5 mg/l BAPe0
Not reportedTaxon
Verbatim
e0“Petiole explants taken from seedlings pretreated with 1 mg/l BAP produced significantly more shoots (0.97 shoot per explant) than those taken from seedlings pretreated with 3 or 5 mg/l BAP (0.68 and 0.59 shoot per explant, respectively).”
Claim · model-written summary
Pretreatment with 1 mg/l BAP produced more shoots per explant than 5 mg/l BAP.
- Treatment
- 1 mg/l BAPe0
- Outcome
- shootse0
- Direction
- moree0
- Value
- 0.97 shoot per explante0
- Comparator
- 5 mg/l BAPe0
Not reportedTaxon
Verbatim
e0“Petiole explants taken from seedlings pretreated with 1 mg/l BAP produced significantly more shoots (0.97 shoot per explant) than those taken from seedlings pretreated with 3 or 5 mg/l BAP (0.68 and 0.59 shoot per explant, respectively).”
Claim · model-written summary
Lower BAP and NAA concentrations in RGL medium produced more shoots than RGH medium.
- Treatment
- RGL mediume0
- Outcome
- direct shoot productione0
- Direction
- more effectivee0
- Value
- 1 shoot per explante0
- Qualifier
- 0.48e0
- Comparator
- RGH mediume0
Not reportedTaxon
Verbatim
e0“The RGL medium containing lower BAP and NAA was significantly more effective in direct shoot production than the RGH medium containing higher BAP and NAA, 1 shoot per explant compared to 0.48, respectively.”
Claim · model-written summary
NAA at 3 mg/l rooted 21.1 percent of regenerated shoots versus 4.1 percent with 1 mg/l NAA.
- Treatment
- 3 mg/l NAAe0
- Outcome
- regenerated shoots developed rootse0
- Direction
- onlye0
- Value
- 21.1%e0
- Qualifier
- 4.1%e0
- Comparator
- 1 mg/l NAAe0
Not reportedTaxon
Verbatim
e0“21.1% of the regenerated shoots developed roots on medium containing 3 mg/l NAA whereas only 4.1% of the shoots rooted on medium containing 1 mg/l NAA.”
Claim · model-written summary
Genotype significantly affected shoot regeneration and rooting.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise1
- Material
- breeding linese1
- Outcome
- shoot regeneration and rootinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genotypic variation was significantly evident for both shoot regeneration and rooting.”
e1“three sugar beet (Beta vulgaris L.) breeding lines”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.