Paper · 1983 · Journal of Experimental Botany

The Effects of δ-Aminolevulinic Acid and Inhibitors of RNA and Protein Synthesis on Phytochrome Mediated Chlorophyll Accumulation inSorghum bicolor

V. K. RAJASEKHAR; SIPRA GUHA-MUKHERJEE; SUDHIR K. SOPORY

8
claims
15
verbatim quotes
5
citations
1
research areas
—
Not open access

Abstract

In 5-d-old etiolated seedlings of Sorghum bicolor, 12 h of darkness after 5 min in red light eliminated a lag before the accumulation of chlorophylls in subsequent continuous white light. Increasing the dark period to 24 h and 36 h, increased the rate of chlorophyll accumulation in the later stages of greening. Exogenous δ-aminolevulinic acid neither completely removed the lag, nor increased the rate of chlorophyll accumulation. Cycloheximide (25 μg ml−1) and 6-methyl purine (5.0 μg ml−1), given continuously or only until the 12 h dark period following the red light irradiation, restored the lag and decreased the rate of chlorophyll accumulation. D-threo-chloramphenicol (400μg ml−1) also decreased the rate of chlorophyll accumulation but did not restore the lag. Addition of these inhibitors even 12 h after red light irradiation decreased the rate of chlorophyll accumulation. Rifampicin (Rifamycin SV, 400 μg ml−1) did not have such effects.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Twelve hours of darkness after red light eliminated the lag in later chlorophyll accumulation.

Taxon
Sorghum bicolorSorghum bicolore0
Organ / stage
5-d-old etiolated seedlingse0
Treatment
12 h of darkness after 5 min in red lighte0
Outcome
a lag before the accumulation of chlorophyllse0
Direction
eliminatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In 5-d-old etiolated seedlings of Sorghum bicolor, 12 h of darkness after 5 min in red light eliminated a lag before the accumulation of chlorophylls in subsequent continuous white light”

✓ located verbatim in the source · abstract, characters 0–186 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Extending darkness to 24 and 36 hours increased later chlorophyll accumulation rates.

Taxon
Sorghum bicolorSorghum bicolort0
Treatment
24 h and 36 he0
Outcome
rate of chlorophyll accumulatione0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Increasing the dark period to 24 h and 36 h, increased the rate of chlorophyll accumulation in the later stages of greening”

✓ located verbatim in the source · abstract, characters 188–311 · show in text ↑
t0

“Sorghum bicolor”

✓ located verbatim in the source · title, characters 132–147 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Exogenous δ-aminolevulinic acid did not completely remove the lag or increase chlorophyll accumulation rate.

Taxon
Sorghum bicolorSorghum bicolort0
Treatment
Exogenous δ-aminolevulinic acide0
Outcome
lage0
Direction
neither completely removede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Exogenous δ-aminolevulinic acid neither completely removed the lag, nor increased the rate of chlorophyll accumulation”

✓ located verbatim in the source · abstract, characters 313–431 · show in text ↑
t0

“Sorghum bicolor”

✓ located verbatim in the source · title, characters 132–147 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cycloheximide restored the lag and decreased chlorophyll accumulation rate.

Taxon
Sorghum bicolorSorghum bicolort0
Treatment
Cycloheximide (25 μg ml−1)e0
Outcome
lag and decreased the rate of chlorophyll accumulatione0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Cycloheximide (25 μg ml−1) and 6-methyl purine (5.0 μg ml−1), given continuously or only until the 12 h dark period following the red light irradiation, restored the lag and decreased the rate of chlorophyll accumulation”

✓ located verbatim in the source · abstract, characters 433–653 · show in text ↑
t0

“Sorghum bicolor”

✓ located verbatim in the source · title, characters 132–147 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

6-methyl purine restored the lag and decreased chlorophyll accumulation rate.

Taxon
Sorghum bicolorSorghum bicolort0
Treatment
6-methyl purine (5.0 μg ml−1)e0
Outcome
rate of chlorophyll accumulatione0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Cycloheximide (25 μg ml−1) and 6-methyl purine (5.0 μg ml−1), given continuously or only until the 12 h dark period following the red light irradiation, restored the lag and decreased the rate of chlorophyll accumulation”

✓ located verbatim in the source · abstract, characters 433–653 · show in text ↑
t0

“Sorghum bicolor”

✓ located verbatim in the source · title, characters 132–147 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

D-threo-chloramphenicol decreased chlorophyll accumulation rate without restoring the lag.

Taxon
Sorghum bicolorSorghum bicolort0
Treatment
D-threo-chloramphenicol (400μg ml−1)e0
Outcome
rate of chlorophyll accumulatione0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“D-threo-chloramphenicol (400μg ml−1) also decreased the rate of chlorophyll accumulation but did not restore the lag”

✓ located verbatim in the source · abstract, characters 655–771 · show in text ↑
t0

“Sorghum bicolor”

✓ located verbatim in the source · title, characters 132–147 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Inhibitors added 12 hours after red irradiation decreased chlorophyll accumulation rate.

Taxon
Sorghum bicolorSorghum bicolort0
Treatment
these inhibitorse0
Outcome
rate of chlorophyll accumulatione0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Addition of these inhibitors even 12 h after red light irradiation decreased the rate of chlorophyll accumulation”

✓ located verbatim in the source · abstract, characters 773–886 · show in text ↑
t0

“Sorghum bicolor”

✓ located verbatim in the source · title, characters 132–147 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Rifampicin did not have the reported lag and chlorophyll accumulation effects.

Taxon
Sorghum bicolorSorghum bicolort0
Treatment
Rifampicin (Rifamycin SV, 400 μg ml−1)e0
Outcome
such effectse0
Direction
did not havee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Rifampicin (Rifamycin SV, 400 μg ml−1) did not have such effects”

✓ located verbatim in the source · abstract, characters 888–952 · show in text ↑
t0

“Sorghum bicolor”

✓ located verbatim in the source · title, characters 132–147 · show in text ↑

light · molecular: metabolites · Sorghum bicolor (sorghum)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.