Paper · 1991 · Australian Journal of Experimental Agriculture

Using omethoate insecticide and legume inoculant on seed

J Evans; J Seidel; GE O'Connor; et al.

12
claims
20
verbatim quotes
20
citations
1
research areas
—
Not open access

Abstract

Omethoate, an organophosphorus insecticide used for early control of redlegged earth mite on legume seed, was toxic to some Rhizobium strains on direct contact when diffused through agar seeded with these bacteria or mixed in broth cultures containing the bacteria. Omethoate mixed with peat-based legume inoculant and applied to seed of subterranean clover or lucerne significantly (P<0.05) reduced the number of nodules formed over 3 weeks on seedlings grown in pots of sand, compared with inoculated controls. Most nodules were immature and located on lateral roots, and plant dry matter at 4 weeks was significantly (P<0.05) less for lucerne. Significantly (P<0.05) fewer rhizobia were recovered from seeds and seedlings of lucerne when seed was treated with omethoate. When subterranean clover seed was pretreated with omethoate 16 h before inoculation and sown into moist sand, there were no significant differences in the mean numbers of rhizobia, mean numbers of nodules or dry matter compared with inoculated controls. With lucerne, the number of nodules formed following omethoate pretreatment was significantly less than on inoculated controls at day 21 but not at day 30, and the dry matter at day 30 was not adversely affected. Rhizobia numbers were reduced less than by mixing omethoate and inoculant, so more taproot nodulation occurred and more of the nodules were functional (i.e. N2-fixing) at 3 weeks. In a field trial with pea, white clover and murex medic, seed pretreatment with omethoate before inoculation had no effect on nodule number 9-11 weeks after sowing, compared with inoculated controls.

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Claims 12

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Omethoate was toxic to some Rhizobium strains on direct contact.

Taxon
legumet0
Treatment
Omethoatee1
Interactor
RhizobiumRhizobiume1symbiont
Outcome
toxic to some Rhizobium strainse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rhizobium
Rhizobium · Interactor · picture of the genus (one of its species)Ninjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Omethoate, an organophosphorus insecticide used for early control of redlegged earth mite on legume seed, was toxic to some Rhizobium strains on direct contact”

✓ located verbatim in the source · abstract, characters 0–159 · show in text ↑
t0

“Using omethoate insecticide and legume inoculant on seed”

✓ located verbatim in the source · title, characters 0–56 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Omethoate applied to seed with peat-based inoculant significantly reduced nodule number on subterranean clover and lucerne seedlings compared with inoculated controls.

Taxon
subterranean clover or lucerneMedicago sativaTrifolium subterraneume2
Treatment
Omethoate mixed with peat-based legume inoculante2
Outcome
number of nodulese2
Direction
reducede2
Qualifier
P<0.05e2
Comparator
inoculated controlse2
Setting
pots of sande2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗
Trifolium subterraneum
Trifolium subterraneum · TaxonKevin Thiele from Perth, Australia · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e2

“Omethoate mixed with peat-based legume inoculant and applied to seed of subterranean clover or lucerne significantly (P<0.05) reduced the number of nodules formed over 3 weeks on seedlings grown in pots of sand, compared with inoculated controls”

✓ located verbatim in the source · abstract, characters 266–511 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Omethoate seed treatment significantly reduced rhizobia recovered from lucerne seeds and seedlings.

Taxon
lucerneMedicago sativae3
Treatment
seed was treated with omethoatee3
Interactor
rhizobiae3symbiont
Outcome
rhizobia were recovered from seeds and seedlingse3
Direction
fewere3
Qualifier
P<0.05e3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e3

“Significantly (P<0.05) fewer rhizobia were recovered from seeds and seedlings of lucerne when seed was treated with omethoate”

✓ located verbatim in the source · abstract, characters 647–772 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Omethoate with inoculant significantly reduced dry matter at 4 weeks in lucerne.

Taxon
lucerneMedicago sativae4
Treatment
Omethoate mixed with peat-based legume inoculante2
Outcome
plant dry mattere4
Direction
lesse4
Qualifier
at 4 weekse4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e4

“plant dry matter at 4 weeks was significantly (P<0.05) less for lucerne”

✓ located verbatim in the source · abstract, characters 574–645 · show in text ↑
e2

“Omethoate mixed with peat-based legume inoculant and applied to seed of subterranean clover or lucerne significantly (P<0.05) reduced the number of nodules formed over 3 weeks on seedlings grown in pots of sand, compared with inoculated controls”

✓ located verbatim in the source · abstract, characters 266–511 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Pretreating subterranean clover seed with omethoate before inoculation had no significant effect on rhizobia, nodules or dry matter.

Taxon
subterranean cloverTrifolium subterraneume5
Treatment
pretreated with omethoate 16 h before inoculatione5
Interactor
rhizobiae5symbiont
Outcome
mean numbers of rhizobia, mean numbers of nodules or dry mattere5
Direction
no_changeinferrede5
Comparator
inoculated controlse5
Setting
moist sande5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium subterraneum
Trifolium subterraneum · TaxonKevin Thiele from Perth, Australia · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e5

“When subterranean clover seed was pretreated with omethoate 16 h before inoculation and sown into moist sand, there were no significant differences in the mean numbers of rhizobia, mean numbers of nodules or dry matter compared with inoculated controls”

✓ located verbatim in the source · abstract, characters 774–1026 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

With lucerne, omethoate pretreatment significantly reduced nodule number compared with inoculated controls at day 21.

Taxon
lucerneMedicago sativae6
Treatment
omethoate pretreatmente6
Outcome
the number of nodules formede6
Direction
lesse6
Qualifier
at day 21e6
Comparator
inoculated controlse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e6

“With lucerne, the number of nodules formed following omethoate pretreatment was significantly less than on inoculated controls at day 21 but not at day 30, and the dry matter at day 30 was not adversely affected”

✓ located verbatim in the source · abstract, characters 1028–1239 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

With lucerne, omethoate pretreatment did not significantly change nodule number at day 30.

Taxon
lucerneMedicago sativae6
Treatment
omethoate pretreatmente6
Outcome
the number of nodules formede6
Direction
no_changeinferrede6
Qualifier
at day 30e6
Comparator
inoculated controlse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e6

“With lucerne, the number of nodules formed following omethoate pretreatment was significantly less than on inoculated controls at day 21 but not at day 30, and the dry matter at day 30 was not adversely affected”

✓ located verbatim in the source · abstract, characters 1028–1239 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

With lucerne, omethoate pretreatment did not adversely affect dry matter at day 30.

Taxon
lucerneMedicago sativae6
Treatment
omethoate pretreatmente6
Outcome
the dry mattere7
Direction
not adversely affectede7
Qualifier
at day 30e7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e6

“With lucerne, the number of nodules formed following omethoate pretreatment was significantly less than on inoculated controls at day 21 but not at day 30, and the dry matter at day 30 was not adversely affected”

✓ located verbatim in the source · abstract, characters 1028–1239 · show in text ↑
e7

“and the dry matter at day 30 was not adversely affected”

✓ located verbatim in the source · abstract, characters 1184–1239 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pretreatment reduced rhizobia numbers less than mixing omethoate and inoculant.

Taxon
subterranean cloverTrifolium subterraneume5
Treatment
pretreated with omethoate 16 h before inoculatione5
Outcome
Rhizobia numberse0
Direction
reduced lesse0
Comparator
mixing omethoate and inoculante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium subterraneum
Trifolium subterraneum · TaxonKevin Thiele from Perth, Australia · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e5

“When subterranean clover seed was pretreated with omethoate 16 h before inoculation and sown into moist sand”

✓ located verbatim in the source · abstract, characters 774–882 · show in text ↑
e0

“Rhizobia numbers were reduced less than by mixing omethoate and inoculant”

✓ located verbatim in the source · abstract, characters 1241–1314 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pretreatment gave more taproot nodulation than mixing omethoate and inoculant.

Taxon
subterranean cloverTrifolium subterraneume5
Treatment
pretreated with omethoate 16 h before inoculatione5
Outcome
taproot nodulatione1
Direction
moree1
Comparator
mixing omethoate and inoculante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium subterraneum
Trifolium subterraneum · TaxonKevin Thiele from Perth, Australia · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e5

“When subterranean clover seed was pretreated with omethoate 16 h before inoculation and sown into moist sand”

✓ located verbatim in the source · abstract, characters 774–882 · show in text ↑
e0

“Rhizobia numbers were reduced less than by mixing omethoate and inoculant”

✓ located verbatim in the source · abstract, characters 1241–1314 · show in text ↑
e1

“so more taproot nodulation occurred”

✓ located verbatim in the source · abstract, characters 1316–1351 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pretreatment gave more functional nodules at 3 weeks than mixing omethoate and inoculant.

Taxon
subterranean cloverTrifolium subterraneume5
Treatment
pretreated with omethoate 16 h before inoculatione5
Outcome
nodules were functional (i.e. N2-fixing)e1
Direction
moree1
Qualifier
at 3 weekse1
Comparator
mixing omethoate and inoculante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium subterraneum
Trifolium subterraneum · TaxonKevin Thiele from Perth, Australia · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e5

“When subterranean clover seed was pretreated with omethoate 16 h before inoculation and sown into moist sand”

✓ located verbatim in the source · abstract, characters 774–882 · show in text ↑
e0

“Rhizobia numbers were reduced less than by mixing omethoate and inoculant”

✓ located verbatim in the source · abstract, characters 1241–1314 · show in text ↑
e1

“more of the nodules were functional (i.e. N2-fixing) at 3 weeks”

✓ located verbatim in the source · abstract, characters 1356–1419 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

In a field trial, seed pretreatment with omethoate before inoculation had no effect on nodule number in pea, white clover and murex medic.

Taxon
pea, white clover and murex medice0
Treatment
seed pretreatment with omethoate before inoculatione0
Outcome
nodule numbere0
Direction
no_changeinferrede0
Qualifier
9-11 weeks after sowinge0
Comparator
inoculated controlse0
Setting
field triale0

Verbatim

e0

“In a field trial with pea, white clover and murex medic, seed pretreatment with omethoate before inoculation had no effect on nodule number 9-11 weeks after sowing, compared with inoculated controls”

✓ located verbatim in the source · abstract, characters 1421–1619 · show in text ↑

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