Abstract
The bacterium capable of efficiently digesting chitin was isolated from chitin-amended field soil. It was identified as Kurthia zopfii, and extracellular chitinases secreted into respective culture filtrates were electrophoretically analyzed using a polyacrylamide gel containing glycol chitin. The result indicated that K. zopfii produced the 72, 58, and 44 kilodalton chitinases. The gene (chiSH1) encoding chitinase was cloned from K. zopfii KI2-119 and the entire nucleotide sequence (2097 bases) was determined. In order to evaluate the feasibility of chitinolytic microbes on the protection of chitinous fungal diseases, viable cells of E. coli transformed with the chiSH1 were applied to barley leaves inoculated with the powdery mildew pathogen. The result indicated that the growth of the pathogen was effectively suppressed by the present treatment, suggesting the importance and effectiveness of chitinolytic microbes as a biocontrol agent.
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Claims 4
Claim · model-written summary
The isolated bacterium was identified as Kurthia zopfii.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Interactor
- Kurthia zopfiiKurthia zopfiie0biocontrol_agent
- Outcome
- identified as Kurthia zopfiie0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“It was identified as Kurthia zopfii”
t0“Barley”
Claim · model-written summary
K. zopfii produced 72, 58, and 44 kilodalton chitinases.
- Taxon
- barleyHordeum vulgaret0
- Interactor
- K. zopfiiKurthia zopfiie0biocontrol_agent
- Outcome
- K. zopfii produced the 72, 58, and 44 kilodalton chitinasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“K. zopfii produced the 72, 58, and 44 kilodalton chitinases”
t0“Barley”
Claim · model-written summary
The chiSH1 chitinase gene was cloned and its 2097-base sequence determined.
- Taxon
- barleyHordeum vulgaret0
- Interactor
- K. zopfii KI2-119Kurthia zopfiie0biocontrol_agent
- Gene
- chiSH1e0
- Outcome
- entire nucleotide sequencee0
- Value
- 2097 basese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene (chiSH1) encoding chitinase was cloned from K. zopfii KI2-119 and the entire nucleotide sequence (2097 bases) was determined”
t0“Barley”
Claim · model-written summary
Viable transformed E. coli cells effectively suppressed powdery mildew pathogen growth on barley leaves.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- viable cells of E. coli transformed with the chiSH1e0
- Interactor
- powdery mildew pathogene0pathogen
- Second interactor
- E. coli
.jpg?width=60)
Escherichia colie0biocontrol_agent - Gene
- chiSH1e0
- Outcome
- growth of the pathogene0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“viable cells of E. coli transformed with the chiSH1 were applied to barley leaves inoculated with the powdery mildew pathogen. The result indicated that the growth of the pathogen was effectively suppressed by the present treatment”
The same questions in other papers
transgenic · growth · Hordeum vulgare (barley)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.