Abstract
BACKGROUND: Optical and spectroscopic technologies working at subcellular resolution with quantitative output are required for a deeper understanding of molecular processes and mechanisms in living cells. Such technologies are prerequisite for the realisation of predictive biology at cellular and subcellular level. However, although established in the physical sciences, these techniques are rarely applied to cell biology in the plant sciences. PRINCIPAL FINDINGS: Here, we present a combined application of one-chromophore fluorescence lifetime microscopy and wavelength-selective fluorescence microscopy to analyse the function of a GFP fusion of the Brassinosteroid Insensitive 1 Receptor (BRI1-GFP) with high spatial and temporal resolution in living Arabidopsis cells in their tissue environment. We show a rapid, brassinolide-induced cell wall expansion and a fast BR-regulated change in the BRI1-GFP fluorescence lifetime in the plasmamembrane in vivo. Both cell wall expansion and changes in fluorescence lifetime reflect early BR-induced and BRI1-dependent physiological or signalling processes. Our experiments also show the potential of one-chromophore fluorescence lifetime microscopy for the in vivo monitoring of the biochemical and biophysical subcellular environment using GFP fusion proteins as probes. SIGNIFICANCE: One-chromophore fluorescence lifetime microscopy, combined with wavelength-specific fluorescence microscopy, opens up new frontiers for in vivo dynamic and quantitative analysis of cellular processes at high resolution which are not addressable by pure imaging technologies or transmission electron microscopy.
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Claims 3
Claim · model-written summary
Brassinolide rapidly induced cell wall expansion in Arabidopsis cells.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- brassinolidee0
- Outcome
- cell wall expansione0
- Direction
- expansione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We show a rapid, brassinolide-induced cell wall expansion”
t0“Arabidopsis”
Claim · model-written summary
Brassinolide rapidly changed BRI1-GFP fluorescence lifetime in the plasma membrane.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- plasmamembranee0
- Treatment
- BRe0
- Gene
- BRI1-GFPe0
- Outcome
- fluorescence lifetimee0
- Direction
- changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a fast BR-regulated change in the BRI1-GFP fluorescence lifetime in the plasmamembrane in vivo”
t0“Arabidopsis”
Claim · model-written summary
One-chromophore fluorescence lifetime microscopy monitored the subcellular biochemical and biophysical environment in living plant tissue.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- in vivo monitoring of the biochemical and biophysical subcellular environmente0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our experiments also show the potential of one-chromophore fluorescence lifetime microscopy for the in vivo monitoring of the biochemical and biophysical subcellular environment using GFP fusion proteins as probes.”
t0“Arabidopsis”
The same questions in other papers
brassinolide · cytology · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.