Abstract
Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction pathways in eukaryotic cells. In tobacco, two MAPK, wound-induced protein kinase (WIPK) and salicylic acid (SA)-induced protein kinase (SIPK), are activated by biotic and abiotic stresses. Both WIPK and SIPK positively regulate the biosynthesis of jasmonic acid (JA) or ethylene (ET) while negatively regulating SA accumulation. We showed previously that recombinant tobacco MAPK phosphatase (NtMKP1) protein dephosphorylates and inactivates SIPK in vitro, and overexpression of NtMKP1 repressed wound-induced activation of both SIPK and WIPK. To elucidate the role of NtMKP1 in response to biotic and abiotic stresses, we generated transgenic tobacco plants in which NtMKP1 expression was suppressed. Suppression of NtMKP1 expression resulted in enhanced activation of WIPK and SIPK and production of both JA and ET upon wounding. Wound-induced expression of JA- or ET-inducible genes, basic PR-1 and PI-II, was also significantly enhanced in these plants. Furthermore, NtMKP1-suppressed plants exhibited enhanced resistance against a necrotrophic pathogen, Botrytis cinerea, and lepidopteran herbivores, Mamestra brassicae and Spodoptera litura. These results suggest that NtMKP1 negatively regulates wound response and resistance against both necrotrophic pathogens and herbivorous insects through suppression of JA or ET pathways via inactivation of MAPK.
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Claims 7
Claim · model-written summary
NtMKP1 suppression enhanced wound-triggered WIPK and SIPK activation in tobacco.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- Suppression of NtMKP1 expressione0
- Gene
- NtMKP1e0
- Outcome
- activation of WIPK and SIPKe0
- Direction
- enhancede0
- Qualifier
- upon woundinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of NtMKP1 expression resulted in enhanced activation of WIPK and SIPK and production of both JA and ET upon wounding”
e1“transgenic tobacco plants in which NtMKP1 expression was suppressed”
Claim · model-written summary
NtMKP1 suppression enhanced wound-triggered JA and ET production in tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Suppression of NtMKP1 expressione0
- Outcome
- production of both JA and ETe0
- Direction
- enhancede0
- Qualifier
- upon woundinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of NtMKP1 expression resulted in enhanced activation of WIPK and SIPK and production of both JA and ET upon wounding”
e1“transgenic tobacco plants in which NtMKP1 expression was suppressed”
Claim · model-written summary
NtMKP1-suppressed tobacco significantly enhanced wound-induced basic PR-1 and PI-II expression.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- transgenic tobacco plantse1
- Treatment
- NtMKP1 expression was suppressede1
- Gene
- basic PR-1 and PI-IIe0
- Outcome
- Wound-induced expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wound-induced expression of JA- or ET-inducible genes, basic PR-1 and PI-II, was also significantly enhanced in these plants”
e1“transgenic tobacco plants in which NtMKP1 expression was suppressed”
Claim · model-written summary
NtMKP1-suppressed tobacco had enhanced resistance to Botrytis cinerea.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtMKP1-suppressede0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtMKP1-suppressed plants exhibited enhanced resistance against a necrotrophic pathogen, Botrytis cinerea, and lepidopteran herbivores, Mamestra brassicae and Spodoptera litura”
e1“transgenic tobacco plants in which NtMKP1 expression was suppressed”
Claim · model-written summary
NtMKP1-suppressed tobacco had enhanced resistance to Mamestra brassicae.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtMKP1-suppressede0
- Interactor
- Mamestra brassicae
_-_(imago)%2C_Arnhem%2C_the_Netherlands_-_2.jpg?width=60)
Mamestra brassicaee0herbivore - Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_(imago)%2C_Arnhem%2C_the_Netherlands_-_2.jpg?width=500)
Verbatim
e0“NtMKP1-suppressed plants exhibited enhanced resistance against a necrotrophic pathogen, Botrytis cinerea, and lepidopteran herbivores, Mamestra brassicae and Spodoptera litura”
e1“transgenic tobacco plants in which NtMKP1 expression was suppressed”
Claim · model-written summary
NtMKP1-suppressed tobacco had enhanced resistance to Spodoptera litura.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtMKP1-suppressede0
- Interactor
- Spodoptera litura
Spodoptera liturae0herbivore - Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtMKP1-suppressed plants exhibited enhanced resistance against a necrotrophic pathogen, Botrytis cinerea, and lepidopteran herbivores, Mamestra brassicae and Spodoptera litura”
e1“transgenic tobacco plants in which NtMKP1 expression was suppressed”
Claim · model-written summary
The authors infer that NtMKP1 negatively regulates tobacco wound responses and pathogen and herbivore resistance through JA or ET pathways.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- NtMKP1e0
- Outcome
- wound response and resistancee0
- Qualifier
- through suppression of JA or ET pathways via inactivation of MAPKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtMKP1 negatively regulates wound response and resistance against both necrotrophic pathogens and herbivorous insects through suppression of JA or ET pathways via inactivation of MAPK”
e1“transgenic tobacco plants in which NtMKP1 expression was suppressed”
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