Abstract
A cDNA library was constructed from a cold-acclimated winter-hardy common wheat (Triticum aestivum L.) cultivar 'Mironovska 808'. Using this library and a cold- and light-responsive barley cDNA clone cor14b as a probe, cDNAs of a homologous wheat gene wcor14 were isolated. Two identical cDNAs designated as wcor14a had an open reading frame encoding an acidic (pI = 4.71) and hydrophobic polypeptide with 140 amino acids (MW = 13.5 kDa). The deduced WCOR14a polypeptide showed 70% identity with the barley chloroplast-imported COR14b and had a nearly identical N-terminal, putative chloroplast transit peptide of 51 amino acid residues. Another cDNA clone wcor14b was assumed to encode a polypeptide WCORb which had 5 substitutions and a frame shift in the C-terminal region as compared with WCOR14a. RACE PCR, genomic PCR and Southern blot analyses suggested that wcor14 and its related sequences constitute a small multigene family with and without an intron in the hexaploid wheat genome. Northern blot analysis showed that transcripts of wcor14 accumulated within 3-6 hours of cold acclimation at 4 degrees C and the level reached a maximum at day 3. The transcripts became non-detectable within 3 hours after de-acclimation at room temperature. Contrary to the barley cor14b, a similar level of wcor14 transcripts was detected under the continuous darkness. Neither treatment with NaCl, ABA nor dehydration induced its expression. Based on these results we conclude that wcor14 is a wheat orthologue of the barley cor14b and specifically induced by low temperature.
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Claims 14
Claim · model-written summary
A homologous wheat gene wcor14 was isolated.
- Taxon
- wheat
Triticum aestivume0 - Gene
- wcor14e0
- Outcome
- were isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cDNAs of a homologous wheat gene wcor14 were isolated.”
Claim · model-written summary
wcor14a encoded an acidic, hydrophobic 140-amino-acid polypeptide.
- Taxon
- wheatTriticum aestivume1
- Gene
- wcor14ae0
- Outcome
- acidic (pI = 4.71) and hydrophobic polypeptidee0
- Value
- 140 amino acidse0
- Qualifier
- MW = 13.5 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two identical cDNAs designated as wcor14a had an open reading frame encoding an acidic (pI = 4.71) and hydrophobic polypeptide with 140 amino acids (MW = 13.5 kDa).”
e1“wheat gene wcor14”
Claim · model-written summary
WCOR14a showed 70% identity with barley COR14b.
- Taxon
- wheatTriticum aestivume1
- Gene
- WCOR14ae0
- Outcome
- identity with the barley chloroplast-imported COR14be0
- Value
- 70%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced WCOR14a polypeptide showed 70% identity with the barley chloroplast-imported COR14b”
e1“wheat gene wcor14”
Claim · model-written summary
WCOR14a contained a nearly identical putative chloroplast transit peptide of 51 residues.
- Taxon
- wheatTriticum aestivume1
- Gene
- wcor14e1
- Outcome
- nearly identical N-terminal, putative chloroplast transit peptidee0
- Value
- 51 amino acid residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had a nearly identical N-terminal, putative chloroplast transit peptide of 51 amino acid residues.”
e1“wheat gene wcor14”
Claim · model-written summary
wcor14b was assumed to encode WCORb with five substitutions and a C-terminal frameshift relative to WCOR14a.
- Taxon
- wheatTriticum aestivume1
- Gene
- wcor14be0
- Outcome
- 5 substitutions and a frame shift in the C-terminal regione0
- Comparator
- WCOR14ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Another cDNA clone wcor14b was assumed to encode a polypeptide WCORb which had 5 substitutions and a frame shift in the C-terminal region as compared with WCOR14a.”
e1“wheat gene wcor14”
Claim · model-written summary
wcor14 related sequences form a small multigene family with and without an intron.
- Taxon
- wheatTriticum aestivume0
- Gene
- wcor14e0
- Outcome
- small multigene family with and without an introne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wcor14 and its related sequences constitute a small multigene family with and without an intron in the hexaploid wheat genome.”
Claim · model-written summary
wcor14 transcripts accumulated within 3-6 hours of cold acclimation at 4 degrees C.
- Taxon
- wheatTriticum aestivume1
- Treatment
- cold acclimation at 4 degrees Ce0
- Gene
- wcor14e0
- Outcome
- transcripts of wcor14e0
- Direction
- accumulatede0
- Qualifier
- within 3-6 hourse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcripts of wcor14 accumulated within 3-6 hours of cold acclimation at 4 degrees C”
e1“wheat gene wcor14”
Claim · model-written summary
wcor14 transcript level reached a maximum on day 3 of cold acclimation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the level reached a maximum at day 3.”
e1“transcripts of wcor14 accumulated within 3-6 hours of cold acclimation at 4 degrees C”
e2“wheat gene wcor14”
Claim · model-written summary
wcor14 transcripts became undetectable within three hours after de-acclimation.
- Taxon
- wheatTriticum aestivume1
- Treatment
- de-acclimation at room temperaturee0
- Gene
- wcor14e1
- Outcome
- The transcriptse0
- Qualifier
- within 3 hourse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcripts became non-detectable within 3 hours after de-acclimation at room temperature.”
e1“wheat gene wcor14”
Claim · model-written summary
Continuous darkness yielded a similar wcor14 transcript level, unlike barley cor14b.
- Taxon
- wheatTriticum aestivume1
- Treatment
- continuous darknesse0
- Gene
- wcor14e0
- Outcome
- level of wcor14 transcriptse0
- Direction
- similare0
- Comparator
- barley cor14be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Contrary to the barley cor14b, a similar level of wcor14 transcripts was detected under the continuous darkness.”
e1“wheat gene wcor14”
Claim · model-written summary
NaCl did not induce wcor14 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- NaCle0
- Gene
- wcor14e1
- Outcome
- its expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither treatment with NaCl, ABA nor dehydration induced its expression.”
e1“wheat gene wcor14”
Claim · model-written summary
ABA did not induce wcor14 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- ABAe0
- Gene
- wcor14e1
- Outcome
- its expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither treatment with NaCl, ABA nor dehydration induced its expression.”
e1“wheat gene wcor14”
Claim · model-written summary
Dehydration did not induce wcor14 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- dehydratione0
- Gene
- wcor14e1
- Outcome
- its expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither treatment with NaCl, ABA nor dehydration induced its expression.”
e1“wheat gene wcor14”
Claim · model-written summary
The authors conclude wcor14 is a wheat orthologue of barley cor14b.
- Taxon
- wheatTriticum aestivume1
- Gene
- wcor14e0
- Outcome
- wheat orthologue of the barley cor14be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wcor14 is a wheat orthologue of the barley cor14b”
e1“wheat gene wcor14”
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