Abstract
MsEnod12A and MsEnod12B are two early nodulin genes from alfalfa (Medicago sativa). Differential expression of these genes was demonstrated using a reverse transcription-polymerase chain reaction approach. MsEnod12A RNA was detected only in nodules and not in other plant tissues. In contrast, MsEnod12B transcripts were found in nodules and also at low levels in roots, flowers, stems, and leaves. MsEnod12B expression was enhanced in the root early after inoculation with the microsymbiont Rhizobium meliloti and after treatment with purified Nod factors, whereas MsEnod12A induction was detected only when developing nodules were visible. In situ hybridization showed that in nodules, MsEnod12 expression occurred in the infection zone. In empty Fix- nodules the MsEnod12A transcript level was much reduced, and in spontaneous nodules it was not detectable. These data indicate that MsEnod12B expression in roots is related to the action of Nod factors, whereas MsEnod12A expression is associated with the invasion process in nodules. Therefore, alfalfa possesses different mechanisms regulating MsEnod12A and MsEnod12B expression.
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Claims 10
Claim · model-written summary
MsEnod12A RNA was detected in nodules but not other tissues.
- Taxon
- alfalfa
Medicago sativat0 - Organ / stage
- nodulese0
- Gene
- MsEnod12Ae0
- Outcome
- MsEnod12A RNA was detected only in nodulese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsEnod12A RNA was detected only in nodules and not in other plant tissues.”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
MsEnod12B transcripts occurred in nodules and at low levels in other organs.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsEnod12Be0
- Outcome
- MsEnod12B transcripts were found in nodules and also at low levels in roots, flowers, stems, and leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsEnod12B transcripts were found in nodules and also at low levels in roots, flowers, stems, and leaves.”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
Rhizobium inoculation enhanced root MsEnod12B expression.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- roote0
- Treatment
- inoculation with the microsymbiont Rhizobium melilotie0
- Interactor
- Rhizobium meliloti
Sinorhizobium melilotie0symbiont - Gene
- MsEnod12Be0
- Outcome
- MsEnod12B expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsEnod12B expression was enhanced in the root early after inoculation with the microsymbiont Rhizobium meliloti”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
Purified Nod factors enhanced root MsEnod12B expression.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- treatment with purified Nod factorse0
- Gene
- MsEnod12Be0
- Outcome
- MsEnod12B expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsEnod12B expression was enhanced in the root early after inoculation with the microsymbiont Rhizobium meliloti and after treatment with purified Nod factors”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
MsEnod12A induction appeared only after developing nodules became visible.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsEnod12Ae0
- Outcome
- MsEnod12A induction was detected only when developing nodules were visiblee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsEnod12A induction was detected only when developing nodules were visible.”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
MsEnod12 expression occurred in the nodule infection zone.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsEnod12e0
- Outcome
- MsEnod12 expression occurred in the infection zonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in nodules, MsEnod12 expression occurred in the infection zone.”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
Empty Fix- nodules had much reduced MsEnod12A transcripts.
- Taxon
- alfalfaMedicago sativat0
- Material
- Fix-e0
- Gene
- MsEnod12Ae0
- Outcome
- MsEnod12A transcript levele0
- Direction
- much reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In empty Fix- nodules the MsEnod12A transcript level was much reduced”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
Spontaneous nodules had no detectable MsEnod12A transcript.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- in spontaneous nodules it was not detectablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in spontaneous nodules it was not detectable.”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
The authors associate root MsEnod12B expression with Nod factor action.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsEnod12Be0
- Outcome
- MsEnod12B expression in rootse0
- Qualifier
- action of Nod factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsEnod12B expression in roots is related to the action of Nod factors”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
The authors associate nodule MsEnod12A expression with invasion.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsEnod12Ae0
- Outcome
- MsEnod12A expressione0
- Qualifier
- invasion process in nodulese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsEnod12A expression is associated with the invasion process in nodules”
t0“alfalfa (Medicago sativa)”
The same questions in other papers
Sinorhizobium meliloti · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
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- negative
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See all 5 papers →nodulation factor · molecular: expression · Medicago sativa (alfalfa)Results agree
2 papers in the same direction as this paper
- positive
- positive
inoculation · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.