Abstract
Due to changing climate, flooding (waterlogged soils and submergence) becomes a major problem in agriculture and crop production. In the present study, the effect of waterlogging was investigated on peroxidases of maize (Zea mays L.) leaves. The plants showed typical adaptations to flooding stress, i.e., alterations in chlorophyll a/b ratios and increased basal shoot diameter. Seven peroxidase bands could be detected by first dimension modified SDS-PAGE and 10 bands by first dimension high resolution Clear Native Electrophoresis that altered in dependence on plant development and time of waterlogging. Native isoelectric focusing revealed three acidic to neutral and four alkaline guaiacol peroxidases that could be further separated by high resolution Clear Native Electrophorese in the second dimension. One neutral peroxidase (pI 7.0) appeared to be down-regulated within four hours after flooding, whereas alkaline peroxidases (pI 9.2, 8.0 and 7.8) were up-regulated after 28 or 52 h. Second dimensions revealed molecular masses of 133 kDa and 85 kDa for peroxidases at pI 8.0 and 7.8, respectively. Size exclusion chromatography revealed native molecular masses of 30–58 kDa for peroxidases identified as class III peroxidases and ascorbate peroxidases by mass spectrometry. Possible functions of these peroxidases in flooding stress will be discussed.
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Claims 8
Claim · model-written summary
Flooded maize changed chlorophyll a/b ratios.
Zea maysNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alterations in chlorophyll a/b ratios and increased basal shoot diameter”
t0“Maize Shoots by Flooding Stress”
Claim · model-written summary
Flooded maize increased basal shoot diameter.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alterations in chlorophyll a/b ratios and increased basal shoot diameter”
t0“Maize Shoots by Flooding Stress”
Claim · model-written summary
Peroxidase bands varied with development and waterlogging time.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seven peroxidase bands could be detected by first dimension modified SDS-PAGE and 10 bands by first dimension high resolution Clear Native Electrophoresis that altered in dependence on plant development and time of waterlogging”
t0“Maize Shoots by Flooding Stress”
Claim · model-written summary
Native isoelectric focusing resolved acidic to neutral and alkaline guaiacol peroxidases.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three acidic to neutral and four alkaline guaiacol peroxidases”
t0“Maize Shoots by Flooding Stress”
Claim · model-written summary
A neutral peroxidase was down-regulated within four hours of flooding.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One neutral peroxidase (pI 7.0) appeared to be down-regulated within four hours after flooding”
t0“Maize Shoots by Flooding Stress”
Claim · model-written summary
Alkaline peroxidases were up-regulated after 28 or 52 hours.
- Treatment
- Flooding Stresst0
- Outcome
- alkaline peroxidasese0
- Direction
- up-regulatede0
- Qualifier
- after 28 or 52 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alkaline peroxidases (pI 9.2, 8.0 and 7.8) were up-regulated after 28 or 52 h”
t0“Maize Shoots by Flooding Stress”
Claim · model-written summary
Peroxidases at pI 8.0 and 7.8 had masses of 133 and 85 kDa, respectively.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“molecular masses of 133 kDa and 85 kDa for peroxidases at pI 8.0 and 7.8, respectively”
t0“Maize Shoots by Flooding Stress”
Claim · model-written summary
Size exclusion chromatography found peroxidase masses of 30–58 kDa, with class III and ascorbate peroxidase identifications.
- Gene
- class III peroxidases and ascorbate peroxidasese0
- Outcome
- native molecular massese0
- Value
- 30–58 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“native molecular masses of 30–58 kDa for peroxidases identified as class III peroxidases and ascorbate peroxidases by mass spectrometry”
t0“Maize Shoots by Flooding Stress”
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