Abstract
Genes at two unlinked loci (Tox1A and Tox1B) are required for production of the polyketide T-toxin by Cochliobolus heterostrophus race T, a pathogenic fungus that requires T-toxin for high virulence to maize with T-cytoplasm. Previous work indicated that Tox1A encodes a polyketide synthase (PKS1) required for T-toxin biosynthesis and for high virulence. To identify genes at Tox1B, a wild-type race T cDNA library was screened for genes missing in the genome of a Tox1B deletion mutant. The library was probed, first with a 415-kb NotI restriction fragment from the genome of the Tox1B mutant, then with the corresponding 560-kb fragment from the genome of wild type. Two genes, DEC1 (similar to acetoacetate decarboxylase-encoding genes) and RED1 (similar to genes encoding members of the medium-chain dehydrogenase/reductase superfamily), were recovered. Targeted disruption of DEC1 drastically reduced both T-toxin production and virulence of race T to T-cytoplasm maize, whereas specific inactivation of RED1 had no apparent effect on T-toxin production (as determined by bioassay) or on virulence. DEC1 and RED1 map within 1.5 kb of each other on Tox1B chromosome 6;12 and are unique to the genome of race T, an observation consistent with the hypothesis that these genes were acquired by C. heterostrophus via a horizontal transfer event.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
DEC1 and RED1 were recovered from the Tox1B region.
Not reportedTaxon
Verbatim
e0“Two genes, DEC1 (similar to acetoacetate decarboxylase-encoding genes) and RED1 (similar to genes encoding members of the medium-chain dehydrogenase/reductase superfamily), were recovered.”
t0“T-cytoplasm Maize”
Claim · model-written summary
Disrupting DEC1 greatly reduced T-toxin production and virulence to T-cytoplasm maize.
- Treatment
- Targeted disruption of DEC1e0
- Interactor
- race Te0pathogen
- Gene
- DEC1e0
- Outcome
- T-toxin production and virulence of race Te0
- Direction
- drastically reducede0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Targeted disruption of DEC1 drastically reduced both T-toxin production and virulence of race T to T-cytoplasm maize”
Claim · model-written summary
Inactivating RED1 had no apparent effect on T-toxin production or virulence.
- Treatment
- specific inactivation of RED1e0
- Gene
- RED1e0
- Outcome
- T-toxin production (as determined by bioassay) or on virulencee0
- Direction
- no apparent effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“specific inactivation of RED1 had no apparent effect on T-toxin production (as determined by bioassay) or on virulence”
t0“T-cytoplasm Maize”
Claim · model-written summary
DEC1 and RED1 map within 1.5 kb on Tox1B chromosome 6;12.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DEC1 and RED1 map within 1.5 kb of each other on Tox1B chromosome 6;12”
t0“T-cytoplasm Maize”
Claim · model-written summary
DEC1 and RED1 are unique to race T genome.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are unique to the genome of race T”
t0“T-cytoplasm Maize”
e1“DEC1 and RED1 map within 1.5 kb”
The same questions in other papers
loss of function · disease · Zea mays (maize)Positive and negative results
20 papers in the same direction as this paper · 12 in the opposite direction
- positive
- positive
- positive
29 papers more in this group
See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.