Abstract
Responses of tobacco (Nicotiana tabacum) suspension cells to Cd and Zn were studied in the presence and absence of ligand of Cd-peptide in order to understand the role of this peptide versus other mechanisms in Cd and Zn accumulation and accommodation in plants. With 45 micromolar Cd and 300 micromolar Zn (non-growth-inhibiting levels), metals appeared rapidly within cells, and intracellular Cd and Zn reached medium concentrations after 6 to 10 hours. Cd-peptide was observed in response to Cd after 2 hours, but this form only accounted for approximately 30% of soluble Cd after 24 hours. Peptide was not observed in cells exposed to 300 micromolar Zn for up to 7 days. Organic acid-to-metal stoichiometry indicated that endogenous organic acid content of cells was more than sufficient to complex absorbed metals and no evidence was found for stimulation of organic acid biosynthesis by Cd or Zn. Metal-complexing potential of organic acids for Cd and Zn versus endogenous cations is discussed as is vacuolar-extravacuolar distribution of metals. The absence of Cd-peptide does not limit Cd-accumulation in the system studied. Results suggest that tobacco suspension cells accommodate the presence of non-growth-inhibiting and growth-inhibiting levels of Cd and Zn by sequestration in the vacuole as complexes with endogenous organic acids and that this may be a principal means for accommodation of Cd as well as Zn in the presence and absence of Cd-peptide.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Intracellular Cd reached medium concentrations after 6 to 10 hours.
- Taxon
- tobacco
Nicotiana tabacume1 - Organ / stage
- suspension cellse1
- Treatment
- 45 micromolar Cde0
- Outcome
- intracellular Cde0
- Direction
- reached medium concentrationse0
- Value
- 6 to 10 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With 45 micromolar Cd and 300 micromolar Zn (non-growth-inhibiting levels), metals appeared rapidly within cells, and intracellular Cd and Zn reached medium concentrations after 6 to 10 hours.”
e1“tobacco (Nicotiana tabacum) suspension cells”
Claim · model-written summary
Intracellular Zn reached medium concentrations after 6 to 10 hours.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- suspension cellse1
- Treatment
- 300 micromolar Zne0
- Outcome
- intracellular Cd and Zne0
- Direction
- reached medium concentrationse0
- Value
- 6 to 10 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With 45 micromolar Cd and 300 micromolar Zn (non-growth-inhibiting levels), metals appeared rapidly within cells, and intracellular Cd and Zn reached medium concentrations after 6 to 10 hours.”
e1“tobacco (Nicotiana tabacum) suspension cells”
Claim · model-written summary
Cd-peptide appeared after 2 hours of Cd exposure.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Cde0
- Outcome
- Cd-peptide was observede0
- Value
- 2 hourse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cd-peptide was observed in response to Cd after 2 hours”
e1“tobacco (Nicotiana tabacum) suspension cells”
Claim · model-written summary
Cd-peptide represented approximately 30% of soluble Cd after 24 hours.
- Taxon
- tobaccoNicotiana tabacume2
- Outcome
- soluble Cde0
- Value
- approximately 30%e0
- Qualifier
- after 24 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this form only accounted for approximately 30% of soluble Cd after 24 hours”
e1“Cd-peptide was observed in response to Cd”
e2“tobacco (Nicotiana tabacum) suspension cells”
Claim · model-written summary
Cd-peptide was absent after up to 7 days of Zn exposure.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- 300 micromolar Zne0
- Outcome
- Peptide was not observede0
- Qualifier
- up to 7 dayse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Peptide was not observed in cells exposed to 300 micromolar Zn for up to 7 days.”
e1“Cd-peptide was observed”
e2“tobacco (Nicotiana tabacum) suspension cells”
Claim · model-written summary
Endogenous organic acids were sufficient to complex absorbed metals.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- endogenous organic acid content of cellse0
- Direction
- more than sufficiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous organic acid content of cells was more than sufficient to complex absorbed metals”
e1“tobacco (Nicotiana tabacum) suspension cells”
Claim · model-written summary
Cd or Zn did not stimulate organic acid biosynthesis.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Cd or Zne0
- Outcome
- organic acid biosynthesise0
- Direction
- no evidence was found for stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no evidence was found for stimulation of organic acid biosynthesis by Cd or Zn”
e1“tobacco (Nicotiana tabacum) suspension cells”
Claim · model-written summary
Absence of Cd-peptide did not limit Cd accumulation.
- Taxon
- tobaccoNicotiana tabacume1
- Compound
- Cd-peptidee0
- Outcome
- Cd-accumulatione0
- Direction
- does not limite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The absence of Cd-peptide does not limit Cd-accumulation in the system studied.”
e1“tobacco (Nicotiana tabacum) suspension cells”
Claim · model-written summary
Cells may accommodate Cd and Zn through vacuolar sequestration with endogenous organic acids.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- accommodate the presence of non-growth-inhibiting and growth-inhibiting levels of Cd and Zne0
- Qualifier
- sequestration in the vacuole as complexes with endogenous organic acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco suspension cells accommodate the presence of non-growth-inhibiting and growth-inhibiting levels of Cd and Zn by sequestration in the vacuole as complexes with endogenous organic acids”
e1“tobacco (Nicotiana tabacum) suspension cells”
The same questions in other papers
zinc atom · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
4 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →cadmium atom · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.