Abstract
Mitochondria isolated from pea (Pisum sativum L.) leaves are able to transport the keto acid, oxaloacetate, from the reaction medium into he mitochondrial matrix at high rates. The rate of uptake by the mitochondria was measured as the rate of disappearance of oxaloacetate from the reaction medium as it was reduced by matrix malate dehydrogenase using NADH provided by glycine oxidation. The oxaloacetate transporter was identifed as being distinct from the dicarboxylate and the alpha-ketoglutarate transporters because of its inhibitor sensitivities and its inability to interact with other potential substrates. Phthalonate and phthalate were competitive inhibitors of oxaloacetate transport with K(i) values of 60 micromolar and 2 millimolar, respectively. Butylmalonate, an inhibitor of the dicarboxylate and alpha-ketoglutarate transporters, did not alter the rate of oxaloacetate transport. In addition, a 1000-fold excess of malate, malonate, succinate, alpha-ketoglutarate, or phosphate had little effect on the rate of oxaloacetate transport. The K(m) for the oxaloacetate transporter was about 15 micromolar with a maximum velocity of over 500 nanomoles per milligram mitochondrial protein/min at 25 degrees C. No requirement for a counter ion to move against oxaloacetate was detected and the highest rates of uptake occurred at alkaline pH values. An equivalent transporter has not been reported in animal mitochondria.
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Claims 10
Claim · model-written summary
Pea leaf mitochondria transported oxaloacetate into the matrix at high rates.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- leavese0
- Outcome
- transport the keto acid, oxaloacetatee0
- Direction
- high ratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mitochondria isolated from pea (Pisum sativum L.) leaves are able to transport the keto acid, oxaloacetate, from the reaction medium into he mitochondrial matrix at high rates”
Claim · model-written summary
The oxaloacetate transporter was distinct from dicarboxylate and alpha-ketoglutarate transporters.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- oxaloacetate transporter was identifed as being distincte0
- Comparator
- dicarboxylate and the alpha-ketoglutarate transporterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The oxaloacetate transporter was identifed as being distinct from the dicarboxylate and the alpha-ketoglutarate transporters because of its inhibitor sensitivities and its inability to interact with other potential substrates”
e1“pea (Pisum sativum L.) leaves”
Claim · model-written summary
Phthalonate competitively inhibited oxaloacetate transport with Ki 60 micromolar.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- Phthalonatee0
- Outcome
- oxaloacetate transporte0
- Direction
- competitive inhibitorse0
- Value
- 60 micromolare0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phthalonate and phthalate were competitive inhibitors of oxaloacetate transport with K(i) values of 60 micromolar and 2 millimolar, respectively”
e1“pea (Pisum sativum L.) leaves”
Claim · model-written summary
Phthalate competitively inhibited oxaloacetate transport with Ki 2 millimolar.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- phthalatee0
- Outcome
- oxaloacetate transporte0
- Direction
- competitive inhibitorse0
- Value
- 2 millimolare0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phthalonate and phthalate were competitive inhibitors of oxaloacetate transport with K(i) values of 60 micromolar and 2 millimolar, respectively”
e1“pea (Pisum sativum L.) leaves”
Claim · model-written summary
Butylmalonate did not alter oxaloacetate transport rate.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- Butylmalonatee0
- Outcome
- rate of oxaloacetate transporte0
- Direction
- did not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Butylmalonate, an inhibitor of the dicarboxylate and alpha-ketoglutarate transporters, did not alter the rate of oxaloacetate transport”
e1“pea (Pisum sativum L.) leaves”
Claim · model-written summary
A 1000-fold excess of malate, malonate, succinate, alpha-ketoglutarate or phosphate had little effect on oxaloacetate transport.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- 1000-fold excess of malate, malonate, succinate, alpha-ketoglutarate, or phosphatee0
- Outcome
- rate of oxaloacetate transporte0
- Direction
- little effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 1000-fold excess of malate, malonate, succinate, alpha-ketoglutarate, or phosphate had little effect on the rate of oxaloacetate transport”
e1“pea (Pisum sativum L.) leaves”
Claim · model-written summary
Transporter Km was about 15 micromolar.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- K(m) for the oxaloacetate transportere0
- Value
- about 15 micromolare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The K(m) for the oxaloacetate transporter was about 15 micromolar with a maximum velocity of over 500 nanomoles per milligram mitochondrial protein/min at 25 degrees C”
e1“pea (Pisum sativum L.) leaves”
Claim · model-written summary
Transporter maximum velocity exceeded 500 nanomoles per milligram mitochondrial protein per minute at 25 degrees C.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- maximum velocitye0
- Value
- over 500 nanomoles per milligram mitochondrial protein/mine0
- Qualifier
- at 25 degrees Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a maximum velocity of over 500 nanomoles per milligram mitochondrial protein/min at 25 degrees C”
e1“pea (Pisum sativum L.) leaves”
Claim · model-written summary
No counter ion requirement for oxaloacetate movement was detected.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- requirement for a counter ion to move against oxaloacetatee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No requirement for a counter ion to move against oxaloacetate was detected”
e1“pea (Pisum sativum L.) leaves”
Claim · model-written summary
Oxaloacetate uptake rates were highest at alkaline pH.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- rates of uptakee0
- Direction
- higheste0
- Setting
- alkaline pH valuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest rates of uptake occurred at alkaline pH values”
e1“pea (Pisum sativum L.) leaves”
The same questions in other papers
succinate · nutrient: content · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
malate · nutrient: content · Lathyrus oleraceus (pea)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
phosphorus · nutrient: content · Lathyrus oleraceus (pea)Positive and negative results
2 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.