Abstract
A reliable plant regeneration procedure by organogenesis has been obtained for Spinacia oleracea L. cv. Hybrid 102. The optimum procedure utilises a sequence of three different media. Explants from sterile seedling cotyledons or hypocotyls are incubated for 4 weeks in the dark on medium containing 4.6 µM kinetin and 4.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D). The calli are then transferred to medium without 2,4-D but containing kinetin and gibberellic acid (GA3). From this point on, incubation is in the light. A number of passages (usually three) of 4-6 weeks are required on this medium for shoot formation to occur. Calli destined to form shoots usually first undergo some root proliferation ('rooty calli'). Shoots can be induced to form roots by transfer to hormone-free medium. It is suggested that GA3 stimulates the development of shoot primordia induced by 2,4-D. The presence of kinetin (but not 6-benzylaminopurine) results in more rapid shoot formation.
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Claims 5
Claim · model-written summary
A three-medium procedure reliably regenerated Spinacia oleracea by organogenesis.
- Taxon
- Spinacia oleracea
Spinacia oleraceae0 - Material
- Hybrid 102e0
- Outcome
- plant regeneration procedure by organogenesise0
- Value
- three different mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A reliable plant regeneration procedure by organogenesis has been obtained for Spinacia oleracea L. cv. Hybrid 102. The optimum procedure utilises a sequence of three different media”
Claim · model-written summary
Shoot-forming calli usually first proliferated roots.
- Taxon
- Spinacia oleraceaSpinacia oleraceat0
- Outcome
- root proliferation ('rooty calli')e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calli destined to form shoots usually first undergo some root proliferation ('rooty calli')”
t0“Spinacia oleracea”
Claim · model-written summary
Hormone-free medium induced shoots to form roots.
- Taxon
- Spinacia oleraceaSpinacia oleraceat0
- Treatment
- hormone-free mediume0
- Outcome
- form rootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoots can be induced to form roots by transfer to hormone-free medium”
t0“Spinacia oleracea”
Claim · model-written summary
GA3 was suggested to stimulate shoot primordia development after 2,4-D induction.
- Taxon
- Spinacia oleraceaSpinacia oleraceat0
- Treatment
- GA3e0
- Outcome
- development of shoot primordiae0
- Qualifier
- induced by 2,4-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is suggested that GA3 stimulates the development of shoot primordia induced by 2,4-D”
t0“Spinacia oleracea”
Claim · model-written summary
Kinetin produced more rapid shoot formation than 6-benzylaminopurine.
- Taxon
- Spinacia oleraceaSpinacia oleraceat0
- Treatment
- kinetine0
- Outcome
- shoot formatione0
- Direction
- more rapide0
- Comparator
- 6-benzylaminopurinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of kinetin (but not 6-benzylaminopurine) results in more rapid shoot formation”
t0“Spinacia oleracea”
The same questions in other papers
kinetin · growth · Spinacia oleracea (spinach)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.