Abstract
The Arabidopsis PR-1 gene is one of a suite of genes induced co-ordinately during the onset of systemic acquired resistance (SAR), a plant defense pathway triggered by pathogen infection or exogenous application of chemicals such as salicylic acid (SA) and 2,6-dichloroisonicotinic acid (INA). We have characterized cis-acting regulatory elements in the PR-1 promoter involved in INA induction using deletion analysis, linker-scanning mutagenesis, and in vivo footprinting. Compared to promoter fragments of 815 bp or longer (which show greater than 10-fold inducibility after INA treatment), induction of a 698 bp long promoter fragment is reduced by half and promoter fragments of 621 bp or shorter have lost all inducibility. Additionally, two 10-bp linker-scanning mutations centered at 640 bp and 610 bp upstream from the transcription initiation site are each sufficient to abolish chemical inducibility of a GUS reporter fusion. The -640 linker-scanning mutation encompasses a region highly homologous to recognition sites for transcription factors of the basic leucine zipper class, while the -610 linker-scanning mutation contains a sequence similar to a consensus recognition site for the transcription factor NF-kappa B. Furthermore, several inducible in vivo footprints located at or nearby these motifs demonstrate significant and highly reproducible changes in DNA accessibility following SAR induction. This in vivo signature of protein-DNA interactions after INA induction is tightly correlated with the functionally important regions of the promoter identified by mutation analysis.
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Claims 8
Claim · model-written summary
An 698 bp PR-1 promoter fragment had half the inducibility of fragments at least 815 bp long after INA treatment.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- INA treatmente0
- Gene
- PR-1e1
- Outcome
- induction of a 698 bp long promoter fragmente0
- Direction
- reducede0
- Value
- halfe0
- Comparator
- promoter fragments of 815 bp or longere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to promoter fragments of 815 bp or longer (which show greater than 10-fold inducibility after INA treatment), induction of a 698 bp long promoter fragment is reduced by half”
e1“The Arabidopsis PR-1 gene”
Claim · model-written summary
PR-1 promoter fragments of 621 bp or less lost all inducibility.
- Taxon
- ArabidopsisArabidopsise1
- Material
- promoter fragments of 621 bp or shortere0
- Gene
- PR-1e1
- Outcome
- lost all inducibilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoter fragments of 621 bp or shorter have lost all inducibility”
e1“The Arabidopsis PR-1 gene”
Claim · model-written summary
A 640 bp linker scanning mutation abolished chemical inducibility of a GUS reporter.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- linker-scanning mutations centered at 640 bpe0
- Gene
- PR-1e1
- Outcome
- chemical inducibility of a GUS reporter fusione0
- Direction
- abolishe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two 10-bp linker-scanning mutations centered at 640 bp and 610 bp upstream from the transcription initiation site are each sufficient to abolish chemical inducibility of a GUS reporter fusion”
e1“The Arabidopsis PR-1 gene”
Claim · model-written summary
A 610 bp linker scanning mutation abolished chemical inducibility of a GUS reporter.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- 610 bpe0
- Gene
- PR-1e1
- Outcome
- chemical inducibility of a GUS reporter fusione0
- Direction
- abolishe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two 10-bp linker-scanning mutations centered at 640 bp and 610 bp upstream from the transcription initiation site are each sufficient to abolish chemical inducibility of a GUS reporter fusion”
e1“The Arabidopsis PR-1 gene”
Claim · model-written summary
The 640 bp mutation overlapped a region homologous to basic leucine zipper recognition sites.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PR-1e1
- Outcome
- region highly homologous to recognition sites for transcription factors of the basic leucine zipper classe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The -640 linker-scanning mutation encompasses a region highly homologous to recognition sites for transcription factors of the basic leucine zipper class”
e1“The Arabidopsis PR-1 gene”
Claim · model-written summary
The 610 bp mutation contained a sequence resembling an NF-kappa B recognition site.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PR-1e1
- Outcome
- sequence similar to a consensus recognition site for the transcription factor NF-kappa Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the -610 linker-scanning mutation contains a sequence similar to a consensus recognition site for the transcription factor NF-kappa B”
e1“The Arabidopsis PR-1 gene”
Claim · model-written summary
Inducible footprints near the motifs showed significant and reproducible DNA accessibility changes after SAR induction.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PR-1e1
- Outcome
- changes in DNA accessibilitye0
- Qualifier
- following SAR inductione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several inducible in vivo footprints located at or nearby these motifs demonstrate significant and highly reproducible changes in DNA accessibility following SAR induction”
e1“The Arabidopsis PR-1 gene”
Claim · model-written summary
The in vivo protein DNA interaction signature after INA induction correlated closely with promoter regions found important by mutation analysis.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PR-1e1
- Outcome
- signature of protein-DNA interactionse0
- Comparator
- functionally important regions of the promotere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This in vivo signature of protein-DNA interactions after INA induction is tightly correlated with the functionally important regions of the promoter identified by mutation analysis”
e1“The Arabidopsis PR-1 gene”
The same questions in other papers
2,6-dichloroisonicotinic acid · molecular: expression · ArabidopsisPositive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.