Abstract
The invertase (EC 3.2.1.26) purified from cell walls of dwarf pea stems to homogeneity has a molecular mass of 64 kilodaltons (kD). Poly(A)+RNA was isolated from shoots of dwarf pea plants, and a cDNA library was constructed using lambda gt11 as an expression vector. The expression cDNA library was screened with polyclonal antibodies against pea cell wall invertase. One invertase cDNA clone was characterized as a full-length cDNA with 1,863 base pairs. Compared with other known invertases, one homologous region in the amino acid sequence was found. The conserved motif, Asn-Asp-Pro-Asn-Gly, is located near the N-terminal end of invertase. Northern blot analysis showed that the amount of invertase mRNA (1.86 kb) was rapidly induced to a maximal level 4 h after GA3 treatment, then gradually decreased to the control level. The mRNA level at 4 h in GA3-treated peas was fivefold higher than that of the control group. The maximal increase in activity of pea cell wall invertase elicited by GA3 occcured at 8 h after GA3 treatment. This invertase isoform was shown immunocytochemically to be localized in the cell walls, where a 10-fold higher accumulation occurred in GA3-treated tissue compared with control tissue. This study indicates that the expression of the pea shoot cell-wall invertase gene could be regulated by GA3 at transcriptional and/or translational levels.
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Claims 10
Claim · model-written summary
Purified cell wall invertase had a molecular mass of 64 kD.
- Taxon
- dwarf pea
Lathyrus oleraceuse0 - Gene
- invertasee0
- Outcome
- molecular masse0
- Value
- 64 kilodaltons (kD)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The invertase (EC 3.2.1.26) purified from cell walls of dwarf pea stems to homogeneity has a molecular mass of 64 kilodaltons (kD).”
Claim · model-written summary
An invertase full length cDNA had 1,863 base pairs.
- Taxon
- dwarf peaLathyrus oleraceust0
- Gene
- invertasee0
- Outcome
- full-length cDNAe0
- Value
- 1,863 base pairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One invertase cDNA clone was characterized as a full-length cDNA with 1,863 base pairs.”
t0“Dwarf Pea”
Claim · model-written summary
The invertase amino acid sequence included a homologous region.
- Taxon
- dwarf peaLathyrus oleraceust0
- Gene
- invertasese0
- Outcome
- one homologous regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with other known invertases, one homologous region in the amino acid sequence was found.”
t0“Dwarf Pea”
Claim · model-written summary
The conserved invertase motif lies near the N terminus.
- Taxon
- dwarf peaLathyrus oleraceust0
- Gene
- invertasee0
- Outcome
- Asn-Asp-Pro-Asn-Glye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The conserved motif, Asn-Asp-Pro-Asn-Gly, is located near the N-terminal end of invertase.”
t0“Dwarf Pea”
Claim · model-written summary
GA3 induced invertase mRNA to a maximum at 4 h before it returned to control level.
- Taxon
- dwarf peaLathyrus oleraceust0
- Treatment
- GA3 treatmente0
- Outcome
- invertase mRNAe0
- Direction
- inducede0
- Value
- 1.86 kbe0
- Qualifier
- 4 he0
- Comparator
- control levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Northern blot analysis showed that the amount of invertase mRNA (1.86 kb) was rapidly induced to a maximal level 4 h after GA3 treatment, then gradually decreased to the control level.”
t0“Dwarf Pea”
Claim · model-written summary
GA3 treated peas had fivefold higher invertase mRNA at 4 h than controls.
- Taxon
- peasLathyrus oleraceuse0
- Treatment
- GA3-treatede0
- Outcome
- mRNA levele0
- Direction
- highere0
- Value
- fivefolde0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mRNA level at 4 h in GA3-treated peas was fivefold higher than that of the control group.”
Claim · model-written summary
Invertase activity increased maximally 8 h after GA3.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- GA3 treatmente0
- Outcome
- activity of pea cell wall invertasee0
- Direction
- increasee0
- Qualifier
- 8 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximal increase in activity of pea cell wall invertase elicited by GA3 occcured at 8 h after GA3 treatment.”
Claim · model-written summary
Cell wall invertase accumulated tenfold more in GA3 treated tissue than control.
- Taxon
- dwarf peaLathyrus oleraceust0
- Organ / stage
- cell wallse0
- Treatment
- GA3-treated tissuee0
- Outcome
- accumulatione0
- Direction
- highere0
- Value
- 10-folde0
- Comparator
- control tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This invertase isoform was shown immunocytochemically to be localized in the cell walls, where a 10-fold higher accumulation occurred in GA3-treated tissue compared with control tissue.”
t0“Dwarf Pea”
Claim · model-written summary
Invertase localized to cell walls.
- Taxon
- dwarf peaLathyrus oleraceust0
- Gene
- invertasee0
- Outcome
- localized in the cell wallse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This invertase isoform was shown immunocytochemically to be localized in the cell walls”
t0“Dwarf Pea”
Claim · model-written summary
The authors infer GA3 regulates pea shoot invertase expression at transcriptional or translational level.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- GA3e0
- Outcome
- expression of the pea shoot cell-wall invertase genee0
- Qualifier
- transcriptional and/or translational levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study indicates that the expression of the pea shoot cell-wall invertase gene could be regulated by GA3 at transcriptional and/or translational levels.”
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