Abstract
Modifications to the gaseous envelope by callus during culture in Petri dishes were shown to reduce growth and promote necrosis of several rice (Oryza sativa L.) cultivars. Incubatingcallus under a continuous flow of gas mixtures of known composition suggested that the inhibition of growth was caused by the accumulation of ethylene, the depletion of oxygen and, to a lesser extent, the accumulation of carbon dioxide. In order to evaluate the importance of ethylene accumulation aminoethoxyvinylglycine (AVG), 1-aminocyclopropane-l-carboxylic acid (ACC and silver nitrate (AgNO3), were added to the nutrient medium and ethylene measurements performed during callus culture. Ethylene restricted callus growth particularly under high (35 °C) as compared to moderate (25 °C) temperatures and under illuminated as compared to darkened incubation. Under illuminated incubation at 25 °C AVG (5 mmol m−3) and AgNO°(50 mmol m−3) significantly improvedcallus growth (100 and 60% respectively) while ACC (200 mmol m−3) significantly decreased growth (40%). AVG and AgNO3 were less effective under dark incubation at 25 °C where ethylene production was lower. Furthermore, callus growth was significantly better in large as compared to small culture vessels since the ethylene concentration was diluted and more oxygen was available for respiration. Better control of ethylene and increased oxygen availability could be a way ofproducing healthy callus for the formation of embryogenic tissues of otherwise recalcitrant cultivars of rice (e.g. Indica IR42) and may be a way of improving manipulation of other cereal species.
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Claims 11
Claim · model-written summary
Modifications to the gaseous envelope by callus reduced growth of several rice cultivars.
- Taxon
- rice
Oryza sativae0 - Material
- several rice (Oryza sativa L.) cultivarse0
- Treatment
- Modifications to the gaseous envelope by calluse0
- Outcome
- growthe0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Modifications to the gaseous envelope by callus during culture in Petri dishes were shown to reduce growth and promote necrosis of several rice (Oryza sativa L.) cultivars.”
Claim · model-written summary
Modifications to the gaseous envelope by callus promoted necrosis in several rice cultivars.
- Taxon
- riceOryza sativae0
- Material
- several rice (Oryza sativa L.) cultivarse0
- Treatment
- Modifications to the gaseous envelope by calluse0
- Outcome
- necrosise0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Modifications to the gaseous envelope by callus during culture in Petri dishes were shown to reduce growth and promote necrosis of several rice (Oryza sativa L.) cultivars.”
Claim · model-written summary
Inhibition of rice callus growth is caused by ethylene accumulation, with oxygen depletion and carbon dioxide accumulation as lesser factors.
- Taxon
- RiceOryza sativat0
- Compound
- ethylenee1
- Outcome
- inhibition of growthe1
- Qualifier
- the depletion of oxygene1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the inhibition of growth was caused by the accumulation of ethylene, the depletion of oxygen and, to a lesser extent, the accumulation of carbon dioxide”
t0“Effect of Ethylene and Culture Environment on Rice Callus Proliferation”
Claim · model-written summary
Ethylene restricted rice callus growth more under high (35 °C) than under moderate (25 °C) temperature.
- Taxon
- RiceOryza sativat0
- Treatment
- high (35 °C)e2
- Compound
- Ethylenee2
- Outcome
- callus growthe2
- Direction
- restrictede2
- Comparator
- moderate (25 °C)e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Ethylene restricted callus growth particularly under high (35 °C) as compared to moderate (25 °C) temperatures and under illuminated as compared to darkened incubation.”
t0“Effect of Ethylene and Culture Environment on Rice Callus Proliferation”
Claim · model-written summary
Ethylene restricted rice callus growth more under illuminated than under darkened incubation.
- Taxon
- RiceOryza sativat0
- Treatment
- illuminatede2
- Compound
- Ethylenee2
- Outcome
- callus growthe2
- Direction
- restrictede2
- Comparator
- darkened incubatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Ethylene restricted callus growth particularly under high (35 °C) as compared to moderate (25 °C) temperatures and under illuminated as compared to darkened incubation.”
t0“Effect of Ethylene and Culture Environment on Rice Callus Proliferation”
Claim · model-written summary
AVG (5 mmol m−3) improved rice callus growth under illuminated incubation at 25 °C (100).
- Taxon
- RiceOryza sativat0
- Treatment
- AVG (5 mmol m−3)e3
- Compound
- AVGe3
- Outcome
- callus growthe3
- Direction
- improvede3
- Value
- 100e3
- Setting
- illuminated incubation at 25 °Ce3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Under illuminated incubation at 25 °C AVG (5 mmol m−3) and AgNO°(50 mmol m−3) significantly improvedcallus growth (100 and 60% respectively)”
t0“Effect of Ethylene and Culture Environment on Rice Callus Proliferation”
Claim · model-written summary
AgNO3 (50 mmol m−3) improved rice callus growth under illuminated incubation at 25 °C (60).
- Taxon
- RiceOryza sativat0
- Treatment
- AgNO°(50 mmol m−3)e3
- Outcome
- callus growthe3
- Direction
- improvede3
- Value
- 60e3
- Setting
- illuminated incubation at 25 °Ce3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Under illuminated incubation at 25 °C AVG (5 mmol m−3) and AgNO°(50 mmol m−3) significantly improvedcallus growth (100 and 60% respectively)”
t0“Effect of Ethylene and Culture Environment on Rice Callus Proliferation”
Claim · model-written summary
ACC (200 mmol m−3) decreased rice callus growth by 40% under illuminated incubation at 25 °C.
- Taxon
- RiceOryza sativat0
- Treatment
- ACC (200 mmol m−3)e4
- Compound
- ACCe4
- Outcome
- growthe4
- Direction
- decreasede4
- Value
- 40%e4
- Setting
- illuminated incubation at 25 °Ce3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“while ACC (200 mmol m−3) significantly decreased growth (40%)”
e3“Under illuminated incubation at 25 °C AVG (5 mmol m−3) and AgNO°(50 mmol m−3) significantly improvedcallus growth (100 and 60% respectively)”
t0“Effect of Ethylene and Culture Environment on Rice Callus Proliferation”
Claim · model-written summary
AVG and AgNO3 were less effective under dark incubation at 25 °C, where ethylene production was lower.
- Taxon
- RiceOryza sativat0
- Treatment
- AVG and AgNO3e5
- Compound
- ethylenee5
- Outcome
- effectivee5
- Direction
- lesse5
- Setting
- dark incubation at 25 °Ce5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“AVG and AgNO3 were less effective under dark incubation at 25 °C where ethylene production was lower”
t0“Effect of Ethylene and Culture Environment on Rice Callus Proliferation”
Claim · model-written summary
Rice callus growth was significantly better in large than in small culture vessels, because ethylene was diluted and more oxygen was available.
- Taxon
- RiceOryza sativat0
- Treatment
- largee6
- Compound
- ethylenee6
- Outcome
- callus growthe6
- Direction
- significantly bettere6
- Qualifier
- since the ethylene concentration was diluted and more oxygen was available for respiratione6
- Comparator
- small culture vesselse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“callus growth was significantly better in large as compared to small culture vessels since the ethylene concentration was diluted and more oxygen was available for respiration”
t0“Effect of Ethylene and Culture Environment on Rice Callus Proliferation”
Claim · model-written summary
Better control of ethylene and oxygen availability could produce healthy callus for embryogenic tissue formation in recalcitrant rice cultivars.
- Taxon
- riceOryza sativae7
- Outcome
- healthy callus for the formation of embryogenic tissuese7
- Qualifier
- recalcitrant cultivarse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“healthy callus for the formation of embryogenic tissues of otherwise recalcitrant cultivars of rice”
The same questions in other papers
1-aminocyclopropanecarboxylic acid · growth · Oryza sativa (rice)Positive and negative results
3 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.