Abstract
Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate peroxidase protein(s) was accompanied by rapid changes in the 2-DE protein profiles, over controls. Out of a total of 56 proteins investigated, which were reproducible in repeated experiments, 52 protein spots were visually identified as differentially expressed over controls. Six proteins were N-terminally blocked, and the sequence of 14 proteins could not be determined, whereas 36 proteins were N-terminally and one was internally sequenced. Ozone caused drastic reductions in the major leaf photosynthetic proteins, including the abundantly present ribulose-1, 5-bisphosphate carboxylase/oxygenase, and induction of various defense/stress related proteins. Most prominent change in leaves, within 24 h post-treatment with ozone, was the induced accumulation of a pathogenesis related (PR) class 5 protein, three PR 10 class proteins, ascorbate peroxidase(s), superoxide dismutase, calcium-binding protein, calreticulin, a novel ATP-dependent CLP protease, and an unknown protein. Present results demonstrate the highly damaging effect of ozone on rice seedlings at the level of the proteome.
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Claims 13
Claim · model-written summary
Ozone caused severe visible necrosis in rice leaves.
- Taxon
- rice
Oryza sativat0 - Treatment
- ozonee0
- Outcome
- visible necrotic damage to leafe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drastic visible necrotic damage to leaf by ozone”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Ozone increased ascorbate peroxidase protein in rice leaves.
- Taxon
- riceOryza sativat0
- Treatment
- ozonet0
- Gene
- ascorbate peroxidase protein(s)e0
- Outcome
- ascorbate peroxidase protein(s)e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“consequent increase in ascorbate peroxidase protein(s)”
t0“ozone stress in rice (Oryza sativa L.)”
Claim · model-written summary
Ozone changed the rice 2-DE protein profile relative to controls.
- Taxon
- riceOryza sativat0
- Treatment
- ozonet0
- Outcome
- 2-DE protein profilese0
- Comparator
- controlse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rapid changes in the 2-DE protein profiles, over controls.”
t0“ozone stress in rice (Oryza sativa L.)”
Claim · model-written summary
Fifty-two of 56 rice protein spots were differentially expressed versus controls.
- Taxon
- riceOryza sativat0
- Outcome
- protein spotse0
- Value
- 52 protein spotse0
- Qualifier
- 56 proteins investigatede0
- Comparator
- controlse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Out of a total of 56 proteins investigated, which were reproducible in repeated experiments, 52 protein spots were visually identified as differentially expressed over controls.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Six rice proteins were N-terminally blocked.
- Taxon
- riceOryza sativat0
- Outcome
- proteins were N-terminally blockede0
- Value
- Six proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six proteins were N-terminally blocked”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Sequences of 14 rice proteins could not be determined.
- Taxon
- riceOryza sativat0
- Outcome
- sequence of 14 proteins could not be determinede0
- Value
- 14 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the sequence of 14 proteins could not be determined”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Thirty-six rice proteins were N-terminally sequenced.
- Taxon
- riceOryza sativat0
- Outcome
- proteins were N-terminallye0
- Value
- 36 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“36 proteins were N-terminally and one was internally sequenced.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
One rice protein was internally sequenced.
- Taxon
- riceOryza sativat0
- Outcome
- internally sequencede0
- Value
- onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“36 proteins were N-terminally and one was internally sequenced.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Ozone sharply reduced major leaf photosynthetic proteins.
- Taxon
- riceOryza sativat0
- Treatment
- Ozonee0
- Gene
- ribulose-1, 5-bisphosphate carboxylase/oxygenasee0
- Outcome
- major leaf photosynthetic proteinse0
- Direction
- drastic reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ozone caused drastic reductions in the major leaf photosynthetic proteins, including the abundantly present ribulose-1, 5-bisphosphate carboxylase/oxygenase”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Ozone induced defense and stress related proteins in rice.
- Taxon
- riceOryza sativat0
- Treatment
- ozonet0
- Outcome
- defense/stress related proteinse0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induction of various defense/stress related proteins.”
t0“ozone stress in rice (Oryza sativa L.)”
Claim · model-written summary
Ozone induced PR class 5 and three PR 10 proteins in leaves within 24 hours.
- Taxon
- riceOryza sativat0
- Treatment
- ozonee0
- Outcome
- pathogenesis related (PR) class 5 protein, three PR 10 class proteinse0
- Direction
- induced accumulatione0
- Qualifier
- within 24 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most prominent change in leaves, within 24 h post-treatment with ozone, was the induced accumulation of a pathogenesis related (PR) class 5 protein, three PR 10 class proteins”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Ozone induced antioxidant and calcium-related proteins in leaves within 24 hours.
- Taxon
- riceOryza sativat0
- Treatment
- ozonet0
- Outcome
- ascorbate peroxidase(s), superoxide dismutase, calcium-binding proteine0
- Direction
- induced accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced accumulation of a pathogenesis related (PR) class 5 protein, three PR 10 class proteins, ascorbate peroxidase(s), superoxide dismutase, calcium-binding protein, calreticulin, a novel ATP-dependent CLP protease, and an unknown protein.”
t0“ozone stress in rice (Oryza sativa L.)”
Claim · model-written summary
Ozone induced calreticulin, CLP protease and an unknown protein in rice leaves.
- Taxon
- riceOryza sativat0
- Treatment
- ozonet0
- Outcome
- calreticulin, a novel ATP-dependent CLP protease, and an unknown proteine0
- Direction
- induced accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced accumulation of a pathogenesis related (PR) class 5 protein, three PR 10 class proteins, ascorbate peroxidase(s), superoxide dismutase, calcium-binding protein, calreticulin, a novel ATP-dependent CLP protease, and an unknown protein.”
t0“ozone stress in rice (Oryza sativa L.)”
The same questions in other papers
ozone · physiology: photosynthesis · Oryza sativa (rice)Results differ
26 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
25 papers more in this group
See all 29 papers →ozone · pest · Oryza sativa (rice)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.