Paper · 1969 · PLANT PHYSIOLOGY

Oligomycin Inhibition of Phosphate Uptake and ATP Labeling in Excised Maize Roots

Carolyn Bledsoe; C. V. Cole; Cleon Ross

7
claims
13
verbatim quotes
20
citations
1
research areas
✓
Open access

Abstract

ATP labeling by newly absorbed (32)P in excised maize roots was reduced 34% by the presence of oligomycin during a 4-min uptake period with no reduction in rate of phosphorus absorption. Longer exposure to oligomycin, during pretreatment periods or longer uptake periods, reduced phosphorus absorption and further reduced ATP synthesis. In these tissues it appears that oligomycin inhibits ATP production at the mitochondria, that ATP is the energy source for phosphorus uptake at the plasmalemma, and that a depletion in the ATP supply causes a reduced rate of uptake.

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Oligomycin reduced ATP labeling by newly absorbed phosphorus by 34% during a four-minute uptake.

Taxon
maizeZea mayse0
Organ / stage
rootse0
Treatment
oligomycine0
Outcome
ATP labeling by newly absorbed (32)Pe0
Direction
reducede0
Value
34%e0
Qualifier
4-min uptake periode0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“ATP labeling by newly absorbed (32)P in excised maize roots was reduced 34% by the presence of oligomycin during a 4-min uptake period with no reduction in rate of phosphorus absorption”

✓ located verbatim in the source · abstract, characters 0–185 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Oligomycin did not reduce phosphorus absorption during the four-minute uptake.

Taxon
maizeZea mayse0
Organ / stage
rootse0
Treatment
oligomycine0
Outcome
rate of phosphorus absorptione0
Direction
no reductione0
Qualifier
4-min uptake periode0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“ATP labeling by newly absorbed (32)P in excised maize roots was reduced 34% by the presence of oligomycin during a 4-min uptake period with no reduction in rate of phosphorus absorption”

✓ located verbatim in the source · abstract, characters 0–185 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Longer oligomycin exposure reduced phosphorus absorption.

Taxon
MaizeZea mayst0
Organ / stage
Rootst0
Treatment
Longer exposure to oligomycine0
Outcome
phosphorus absorptione0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Longer exposure to oligomycin, during pretreatment periods or longer uptake periods, reduced phosphorus absorption”

✓ located verbatim in the source · abstract, characters 187–301 · show in text ↑
t0

“Maize Roots”

✓ located verbatim in the source · title, characters 70–81 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Longer oligomycin exposure further reduced ATP synthesis.

Taxon
MaizeZea mayst0
Organ / stage
Rootst0
Treatment
Longer exposure to oligomycine0
Outcome
ATP synthesise0
Direction
further reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Longer exposure to oligomycin, during pretreatment periods or longer uptake periods, reduced phosphorus absorption and further reduced ATP synthesis”

✓ located verbatim in the source · abstract, characters 187–335 · show in text ↑
t0

“Maize Roots”

✓ located verbatim in the source · title, characters 70–81 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors infer that oligomycin inhibits mitochondrial ATP production.

Taxon
MaizeZea mayst0
Organ / stage
Rootst0
Treatment
oligomycine0
Outcome
ATP productione0
Direction
inhibitse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“oligomycin inhibits ATP production at the mitochondria”

✓ located verbatim in the source · abstract, characters 370–424 · show in text ↑
t0

“Maize Roots”

✓ located verbatim in the source · title, characters 70–81 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors infer that ATP supplies energy for phosphorus uptake at the plasmalemma.

Taxon
MaizeZea mayst0
Organ / stage
Rootst0
Compound
ATPe0
Outcome
phosphorus uptakee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“ATP is the energy source for phosphorus uptake at the plasmalemma”

✓ located verbatim in the source · abstract, characters 431–496 · show in text ↑
t0

“Maize Roots”

✓ located verbatim in the source · title, characters 70–81 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors infer that ATP depletion lowers phosphorus uptake rate.

Taxon
MaizeZea mayst0
Organ / stage
Rootst0
Compound
ATPe0
Outcome
rate of uptakee0
Direction
reducede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a depletion in the ATP supply causes a reduced rate of uptake”

✓ located verbatim in the source · abstract, characters 507–568 · show in text ↑
e1

“ATP is the energy source for phosphorus uptake at the plasmalemma”

✓ located verbatim in the source · abstract, characters 431–496 · show in text ↑
t0

“Maize Roots”

✓ located verbatim in the source · title, characters 70–81 · show in text ↑

oligomycin · nutrient: content · Zea mays (maize)Positive and negative results

7 papers: 1 positive, 6 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

oligomycin · molecular: metabolites · Zea mays (maize)Results agree

4 papers: 0 positive, 4 negative or no effect

3 papers in the same direction as this paper

  1. negative
  2. negative
  3. no effect
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.