Abstract
During in vitro growth and maturation of soybean seeds, cessation of embryo growth and dry weight accumulation occurred in the presence of abundant C and N nutrients. Axis followed by cotyledon tissues changed from green to yellow, and post-harvest germination potential declined if cultured after yellowing of axis tissues. A tissue specific accumulat;on of methanol occurred during the in vitro culture of immature seeds (i.e. initially 50 to 70 mg fresh weight) to maturity in liquid medium. Methanol accumulated to 3.0 g m−3 or 50 µg seed−1 in the medium, while methanol decreased from 37 to about 3.0 ∧µg g−1 fresh weight in cotyledons. By contrast, axis tissues increased 20-fold in methanol concentration to 90 µg g−1 during 20 d in culture. Ethanol was present only in trace amounts in axis tissues and medium. Addition of exogenous methanol vapour to in situ grown seeds during precocious maturation decreased subsequent seedling vigour and germination with increasing levels of exposure. Methanol accumulation in axis tissues during the germination phase was not correlated with high temperature and tissue water content treatments which simulated pre-harvest deterioration of seeds. However, the accumulation of methanol during in vitro seed development and maturation in liquid culture may contribute to reduced post-harvest germination performance.
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Claims 12
Claim · model-written summary
Embryo growth and dry weight accumulation ceased despite abundant carbon and nitrogen.
- Taxon
- soybean seeds
Glycine maxt0 - Outcome
- embryo growth and dry weight accumulatione0
- Qualifier
- abundant C and N nutrientse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cessation of embryo growth and dry weight accumulation occurred in the presence of abundant C and N nutrients”
t0“soybean seeds”
Claim · model-written summary
Axis and then cotyledons changed from green to yellow.
- Taxon
- soybean seedsGlycine maxt0
- Outcome
- Axis followed by cotyledon tissues changed from green to yellowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Axis followed by cotyledon tissues changed from green to yellow”
t0“soybean seeds”
Claim · model-written summary
Germination potential declined after axis yellowing.
- Taxon
- soybean seedsGlycine maxt0
- Outcome
- post-harvest germination potentiale0
- Direction
- declinede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“post-harvest germination potential declined if cultured after yellowing of axis tissues”
t0“soybean seeds”
Claim · model-written summary
Methanol accumulated in culture medium to 3.0 g m−3.
- Taxon
- soybean seedsGlycine maxt0
- Outcome
- Methanol accumulatede0
- Value
- 3.0 g m−3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methanol accumulated to 3.0 g m−3 or 50 µg seed−1 in the medium”
t0“soybean seeds”
Claim · model-written summary
Methanol accumulated in culture medium to 50 µg seed−1.
- Taxon
- soybean seedsGlycine maxt0
- Outcome
- Methanol accumulatede0
- Value
- 50 µg seed−1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methanol accumulated to 3.0 g m−3 or 50 µg seed−1 in the medium”
t0“soybean seeds”
Claim · model-written summary
Cotyledon methanol decreased from 37 to about 3.0 ∧µg g−1 fresh weight.
- Taxon
- soybean seedsGlycine maxt0
- Outcome
- methanol decreasede0
- Direction
- decreasede0
- Value
- 37 to about 3.0 ∧µg g−1 fresh weighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“methanol decreased from 37 to about 3.0 ∧µg g−1 fresh weight in cotyledons”
t0“soybean seeds”
Claim · model-written summary
Axis methanol concentration rose twentyfold to 90 µg g−1.
- Taxon
- soybean seedsGlycine maxt0
- Organ / stage
- axis tissuese0
- Outcome
- methanol concentratione0
- Direction
- increasede0
- Value
- 90 µg g−1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“axis tissues increased 20-fold in methanol concentration to 90 µg g−1 during 20 d in culture”
t0“soybean seeds”
Claim · model-written summary
Ethanol occurred only in traces in axis and medium.
- Taxon
- soybean seedsGlycine maxt0
- Outcome
- Ethanol was present only in trace amounts in axis tissues and mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ethanol was present only in trace amounts in axis tissues and medium”
t0“soybean seeds”
Claim · model-written summary
Exogenous methanol vapour decreased subsequent seedling vigour with increasing exposure.
- Taxon
- soybean seedsGlycine maxt0
- Treatment
- exogenous methanol vapoure0
- Outcome
- seedling vigoure0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of exogenous methanol vapour to in situ grown seeds during precocious maturation decreased subsequent seedling vigour and germination with increasing levels of exposure”
t0“soybean seeds”
Claim · model-written summary
Exogenous methanol vapour decreased germination with increasing exposure.
- Taxon
- soybean seedsGlycine maxt0
- Treatment
- exogenous methanol vapoure0
- Outcome
- germinatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of exogenous methanol vapour to in situ grown seeds during precocious maturation decreased subsequent seedling vigour and germination with increasing levels of exposure”
t0“soybean seeds”
Claim · model-written summary
Axis methanol accumulation was uncorrelated with high temperature and tissue water content treatments.
- Taxon
- soybean seedsGlycine maxt0
- Treatment
- high temperature and tissue water content treatmentse0
- Outcome
- Methanol accumulation in axis tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methanol accumulation in axis tissues during the germination phase was not correlated with high temperature and tissue water content treatments”
t0“soybean seeds”
Claim · model-written summary
The authors proposed methanol accumulation may contribute to reduced postharvest germination.
- Taxon
- soybean seedsGlycine maxt0
- Outcome
- reduced post-harvest germination performancee0
- Qualifier
- accumulation of methanole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of methanol during in vitro seed development and maturation in liquid culture may contribute to reduced post-harvest germination performance”
t0“soybean seeds”
The same questions in other papers
methanol · growth · Glycine max (soybean)Results differ
6 in the opposite direction
- positive
- positive
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3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.