Abstract
To determine the requirement for polyamines in gibberellin (GA) induced internode growth polyamine content was measured in internodes of peas of various internode phenotypes (slender, tall, dwarf, nana) with and without applied gibberellin (GA(3)) and polyamine synthesis inhibitors. Polyamines were assayed as dansyl derivatives which were separated by reverse phase high performance liquid chromatography and detected by fluorescence spectrophotometry. The amounts of polyamines in the different genetic lines of peas, which differed in internode lengths and extractable GA content, correlated with the extent of internode elongation. High polyamine concentrations were associated with young internodes and decreased with internode expansion. Extremely short internodes of nana plants without GA exhibited equal or higher amine concentrations relative to internodes of other lines of peas and GA-stimulated nana seedlings. The polyamine synthesis inhibitors, alpha-difluoromethylornithine and alpha-difluoromethylarginine, independently or in combination, inhibited polyamine accumulation and internode elongation of tall peas and GA-stimulated nana plants. Agmatine and putrescine restored growth and endogenous polyamine content to variable degrees. However, exogenous polyamines were not effective in promoting growth unless intracellular amines were partially depleted.These results suggest that polyamines do not have a role in cell elongation, but may be required to support cell proliferation. Polyamines do not mediate the entire action of GA in internode growth of peas since GA induction of growth involves both cell division and cell elongation, whereas polyamines appear to affect cell division only.
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Claims 10
Claim · model-written summary
Polyamine amounts across pea lines correlated with internode elongation.
- Taxon
- peas
Lathyrus oleraceuse0 - Material
- different genetic linese0
- Compound
- polyaminese0
- Outcome
- extent of internode elongatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amounts of polyamines in the different genetic lines of peas, which differed in internode lengths and extractable GA content, correlated with the extent of internode elongation”
Claim · model-written summary
Young internodes had high polyamine concentrations that decreased with expansion.
- Taxon
- peasLathyrus oleraceuse1
- Organ / stage
- young internodese0
- Outcome
- polyamine concentrationse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High polyamine concentrations were associated with young internodes and decreased with internode expansion”
e1“internodes of peas”
Claim · model-written summary
Short nana internodes without GA had equal or higher amine concentrations than other lines and GA-stimulated nana seedlings.
- Taxon
- peasLathyrus oleraceuse0
- Material
- nanae0
- Organ / stage
- internodese0
- Outcome
- amine concentrationse0
- Direction
- equal or highere0
- Comparator
- other lines of pease0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Extremely short internodes of nana plants without GA exhibited equal or higher amine concentrations relative to internodes of other lines of peas and GA-stimulated nana seedlings”
Claim · model-written summary
Alpha-difluoromethylornithine inhibited polyamine accumulation and internode elongation in tall and GA-stimulated nana peas.
- Taxon
- peasLathyrus oleraceuse0
- Treatment
- alpha-difluoromethylornithinee0
- Outcome
- polyamine accumulation and internode elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polyamine synthesis inhibitors, alpha-difluoromethylornithine and alpha-difluoromethylarginine, independently or in combination, inhibited polyamine accumulation and internode elongation of tall peas and GA-stimulated nana plants”
Claim · model-written summary
Alpha-difluoromethylarginine inhibited polyamine accumulation and internode elongation in tall and GA-stimulated nana peas.
- Taxon
- peasLathyrus oleraceuse0
- Treatment
- alpha-difluoromethylargininee0
- Outcome
- polyamine accumulation and internode elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polyamine synthesis inhibitors, alpha-difluoromethylornithine and alpha-difluoromethylarginine, independently or in combination, inhibited polyamine accumulation and internode elongation of tall peas and GA-stimulated nana plants”
Claim · model-written summary
Agmatine restored growth and endogenous polyamine content to variable degrees.
- Taxon
- peasLathyrus oleraceuse1
- Treatment
- Agmatinee0
- Outcome
- growth and endogenous polyamine contente0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agmatine and putrescine restored growth and endogenous polyamine content to variable degrees”
e1“internodes of peas”
Claim · model-written summary
Putrescine restored growth and endogenous polyamine content to variable degrees.
- Taxon
- peasLathyrus oleraceuse1
- Treatment
- putrescinee0
- Outcome
- growth and endogenous polyamine contente0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agmatine and putrescine restored growth and endogenous polyamine content to variable degrees”
e1“internodes of peas”
Claim · model-written summary
Exogenous polyamines did not promote growth unless intracellular amines were partly depleted.
- Taxon
- peasLathyrus oleraceuse1
- Treatment
- exogenous polyaminese0
- Outcome
- growthe0
- Direction
- not effectivee0
- Qualifier
- unless intracellular amines were partially depletede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous polyamines were not effective in promoting growth unless intracellular amines were partially depleted”
e1“internodes of peas”
Claim · model-written summary
Authors infer polyamines may support cell proliferation rather than cell elongation.
- Taxon
- peasLathyrus oleraceuse1
- Outcome
- cell proliferatione0
- Qualifier
- may be required to support cell proliferatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polyamines do not have a role in cell elongation, but may be required to support cell proliferation”
e1“internodes of peas”
Claim · model-written summary
Authors infer polyamines mediate only part of GA-induced internode growth, affecting cell division rather than elongation.
- Taxon
- peasLathyrus oleraceuse0
- Outcome
- internode growthe0
- Qualifier
- polyamines appear to affect cell division onlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Polyamines do not mediate the entire action of GA in internode growth of peas since GA induction of growth involves both cell division and cell elongation, whereas polyamines appear to affect cell division only”
The same questions in other papers
putrescine · growth · Lathyrus oleraceus (pea)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.