Abstract
Alfalfa (Medicagosativa L.) is one of the most widely grown crops in the USA. Phosphate (P) deficiency is common in areas where forage crops are grown. To improve the use of organic phosphate by alfalfa, two Medicagotruncatula genes, phytase (MtPHY1) and purple acid phosphatase (MtPAP1), were overexpressed in alfalfa under the control of the constitutive CaMV35S promoter or the root-specific MtPT1 promoter. Root enzyme activity analyses revealed that although both genes lead to similar levels of acid phosphatase activities, overexpression of the MtPHY1 gene usually results in a higher level of phytase activity than overexpression of the MtPAP1 gene. The MtPT1 promoter was more effective than the CaMV35S promoter in regulating gene expression and extracellular secretion under P-deficient conditions. Measurement of growth performance of the transgenic lines further proved that the best promoter-gene combination is the MtPHY1 gene driven by the MtPT1 promoter. Compared to the control, the plants with high levels of transgene expression showed improved growth. The biomass of several transgenic lines was three times that of the control when plants were grown in sand supplied with phytate as the sole P source. When the plants were grown in natural soils without additional P supplement, the best performing transgenic lines produced double the amount of biomass after 12 weeks (two cuts) of growth. Transgene effects were more obvious in soil with lower pH and lower natural P reserves than in soil with neutral pH and relatively higher P storage. The total P concentration in leaf tissues of the high-expressing transgenic lines was significantly higher than that of the control. The transgenes have great potential for improving plant P acquisition and biomass yield in P-deficient agricultural soils. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-011-9628-0) contains supplementary material, which is available to authorized users.
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Claims 9
Claim · model-written summary
Overexpression of MtPHY1 in alfalfa usually gave a higher phytase activity than overexpression of MtPAP1.
- Taxon
- Alfalfa
Medicago sativae0 - Treatment
- overexpression of the MtPHY1 genee1
- Gene
- MtPHY1e1
- Outcome
- phytase activitye1
- Direction
- highere1
- Comparator
- overexpression of the MtPAP1 genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“overexpression of the MtPHY1 gene usually results in a higher level of phytase activity than overexpression of the MtPAP1 gene”
Claim · model-written summary
Overexpression of both genes led to similar levels of acid phosphatase activity in alfalfa roots.
- Taxon
- AlfalfaMedicago sativae0
- Outcome
- similar levels of acid phosphatase activitiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“both genes lead to similar levels of acid phosphatase activities”
Claim · model-written summary
The MtPT1 promoter was more effective than the CaMV35S promoter in regulating gene expression and extracellular secretion under P-deficient conditions.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- MtPT1 promotere1
- Outcome
- regulating gene expression and extracellular secretione1
- Direction
- more effectivee1
- Comparator
- CaMV35S promotere1
- Setting
- P-deficient conditionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“The MtPT1 promoter was more effective than the CaMV35S promoter in regulating gene expression and extracellular secretion under P-deficient conditions”
Claim · model-written summary
The best promoter-gene combination for growth performance of the transgenic lines is the MtPHY1 gene driven by the MtPT1 promoter.
- Taxon
- AlfalfaMedicago sativae0
- Gene
- MtPHY1e1
- Outcome
- best promoter-gene combinatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“the best promoter-gene combination is the MtPHY1 gene driven by the MtPT1 promoter”
Claim · model-written summary
Compared to the control, alfalfa plants with high levels of transgene expression showed improved growth.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- high levels of transgene expressione1
- Outcome
- growthe1
- Direction
- improvede1
- Comparator
- the controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“Compared to the control, the plants with high levels of transgene expression showed improved growth”
Claim · model-written summary
The biomass of several transgenic alfalfa lines was three times that of the control when grown in sand supplied with phytate as the sole P source.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- transgenic linese1
- Outcome
- biomasse1
- Direction
- three timese1
- Value
- three timese1
- Comparator
- the controle1
- Setting
- sand supplied with phytate as the sole P sourcee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“The biomass of several transgenic lines was three times that of the control when plants were grown in sand supplied with phytate as the sole P source”
Claim · model-written summary
In natural soils without additional P, the best performing transgenic alfalfa lines produced double the biomass after 12 weeks (two cuts) of growth.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- best performing transgenic linese1
- Outcome
- biomasse1
- Direction
- doublee1
- Value
- double the amounte1
- Qualifier
- after 12 weeks (two cuts) of growthe1
- Setting
- natural soils without additional P supplemente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“When the plants were grown in natural soils without additional P supplement, the best performing transgenic lines produced double the amount of biomass after 12 weeks (two cuts) of growth”
Claim · model-written summary
Transgene effects were more obvious in soil with lower pH and lower natural P reserves than in soil with neutral pH and relatively higher P storage.
- Taxon
- AlfalfaMedicago sativae0
- Outcome
- Transgene effectse1
- Direction
- more obviouse1
- Comparator
- soil with neutral pH and relatively higher P storagee1
- Setting
- soil with lower pH and lower natural P reservese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“Transgene effects were more obvious in soil with lower pH and lower natural P reserves than in soil with neutral pH and relatively higher P storage”
Claim · model-written summary
The total P concentration in leaf tissues of the high-expressing transgenic alfalfa lines was significantly higher than that of the control.
- Taxon
- AlfalfaMedicago sativae0
- Organ / stage
- leaf tissuese1
- Treatment
- high-expressing transgenic linese1
- Outcome
- total P concentratione1
- Direction
- highere1
- Comparator
- the controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicagosativa L.)”
e1“The total P concentration in leaf tissues of the high-expressing transgenic lines was significantly higher than that of the control”
The same questions in other papers
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